Characterization of rock bream (Oplegnathus fasciatus) fin cells and its susceptibility to different genotypes of megalocytiviruses
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Genus Megalocytivirus cause red sea bream iridoviral disease (RSIVD) and scale drop disease(SDD). Based on the phylogeny of the major capsid protein (MCP) and adenosine triphosphatase(ATPase) genes, megalocytiviruses except for SDD virus (SDDV) could be three different genotypes,red sea bream iridovirus (RSIV), infectious spleen and kidney necrosis (ISKNV), and turbot reddishbody iridovirus (TRBIV). In this study, primary cells derived from the caudal fin of rock bream(Oplegnathus fasciatus) grew at 25℃ in Leibovitz’s medium supplemented with 10% (v/v) fetal bovineserum and primocin (100 μg/mL). Rock bream fin (RBF) cells exhibited susceptibility to infectionsby different genotypes of megalocytiviruses (RSIV, ISKNV and TRBIV) with the appearance of cytopathiceffects with an increase in the viral genome copy number. Furthermore, compared to gruntfin (GF) cells, even though 10 times lower number of RSIV genome copies were inoculated in RBFcells, viral genome copy number produced on RBF cells were 44 times higher than that of GF cellsat 7 d post-inoculation. As the isolated RBF cells are sensitive to different genotypes of megalocytiviruses(RSIV, ISKNV and TRBIV), they can be used for future studies regarding in vitro viralinfection and subsequent diagnosis.
Abstract
Genus Megalocytivirus cause red sea bream iridoviral disease (RSIVD) and scale drop disease(SDD). Based on the phylogeny of the major capsid protein (MCP) and adenosine triphosphatase(ATPase) genes, megalocytiviruses except for SDD virus (SDDV) could be three different genotypes,red sea bream iridovirus (RSIV), infectious spleen and kidney necrosis (ISKNV), and turbot reddishbody iridovirus (TRBIV). In this study, primary cells derived from the caudal fin of rock bream(Oplegnathus fasciatus) grew at 25℃ in Leibovitz’s medium supplemented with 10% (v/v) fetal bovineserum and primocin (100 μg/mL). Rock bream fin (RBF) cells exhibited susceptibility to infectionsby different genotypes of megalocytiviruses (RSIV, ISKNV and TRBIV) with the appearance of cytopathiceffects with an increase in the viral genome copy number. Furthermore, compared to gruntfin (GF) cells, even though 10 times lower number of RSIV genome copies were inoculated in RBFcells, viral genome copy number produced on RBF cells were 44 times higher than that of GF cellsat 7 d post-inoculation. As the isolated RBF cells are sensitive to different genotypes of megalocytiviruses(RSIV, ISKNV and TRBIV), they can be used for future studies regarding in vitro viralinfection and subsequent diagnosis.
참고문헌 (29)
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