Propagation of lymphocystis disease virus (LCDV) in the FFN cell line originated from olive flounder Paralichthys olivaceus fin
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The present study demonstrated lymphocystis disease virus (LCDV) propagation through cytopathic effects (CPE)formation and LCDV detection in olive flounder fin (FFN) cells by polymerase chain reaction (PCR) and fluorescent antibody technique (FAT) methods. Tissue filtrates from the cluster cells produced CPE in FFN cells, which initially cells became enlarged and gradually underwent fusion en masse. Infectivity of culture grown LCDV using the FFN cells reached 10^(2.3) TCID_(50)/ml at 4 days post infection and the highest titer was measured 10^(6.5) TCID_(50)/ml at 12 days. The viral DNA was detected in the cell culture supernatants showing CPE and the CPE cells by PCR. Antigen specific strong fluorescence reacting with monoclonal antibody against the virus revealed the presence of viral antigen in the cytoplasm of infected FFN cells. These results suggest that the FFN cell line originated from the olive flounder has a susceptibility of the LCDV.
Abstract
The present study demonstrated lymphocystis disease virus (LCDV) propagation through cytopathic effects (CPE)formation and LCDV detection in olive flounder fin (FFN) cells by polymerase chain reaction (PCR) and fluorescent antibody technique (FAT) methods. Tissue filtrates from the cluster cells produced CPE in FFN cells, which initially cells became enlarged and gradually underwent fusion en masse. Infectivity of culture grown LCDV using the FFN cells reached 10^(2.3) TCID_(50)/ml at 4 days post infection and the highest titer was measured 10^(6.5) TCID_(50)/ml at 12 days. The viral DNA was detected in the cell culture supernatants showing CPE and the CPE cells by PCR. Antigen specific strong fluorescence reacting with monoclonal antibody against the virus revealed the presence of viral antigen in the cytoplasm of infected FFN cells. These results suggest that the FFN cell line originated from the olive flounder has a susceptibility of the LCDV.
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