양식 극동산 뱀장어(Anguilla japonica)에서 분리된 Lactococcus lactis의 항생제 내성
The antibiotic resistance of Lactococcus lactis isolated from the farmed Anguilla japonica
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Spleen, liver and intestinal mucus of Anguilla japonica cultured in a domestic recirculating aquaculture system were sampled to isolate and identify culturable bacteria. Based on 16s rRNA sequence analysis, the most frequently isolated bacterium was Lactococcus lactis. To determine the antibiotic susceptibility of L. lactis, the minimum inhibitory concentration (MIC) test was conducted using five isolates (A1, A21, A65, A125 and A130) from Anguilla japonica and three isolates (KCTC2013, KCTC3769 and KCTC3899) from Korean Collection for Type Culture. Differences of MICs between A. japonica-derived isolates and KCTC isolates were observed in Tetracycline, Oxytetracycline, Doxycyclin and Erythromycin. MICs obtained for five L. lactis isolates from A. japonica were >64 μg/mL for Tetracycline, >256 μg/mL for Oxytetracycline, 16-32 μg/mL for Doxycyclin, and > 64μg/mL for Erythromycin. On the other hand, KCTC isolates showed the MICs of 0.25 μg/mL, 0.25 μg/mL, 0.12 μg/mL and 0.03-0.06 μg/mL for respective antibiotics. Furthermore, it was confirmed that genomic DNA and plasmid DNA isolated from A. japonica-derived L. lactis isolates have antibiotic resistance genes and transfer origin. These findings suggest that A. japonica-derived L. lactis isolates may have the potential to mediate the transfer of various antibiotic resistance genes. Therefore, it is advisable to use probiotics along with the feed or use them as postbiotics rather than dispersing them directly into the water in aquaculture system.
Abstract
Spleen, liver and intestinal mucus of Anguilla japonica cultured in a domestic recirculating aquaculture system were sampled to isolate and identify culturable bacteria. Based on 16s rRNA sequence analysis, the most frequently isolated bacterium was Lactococcus lactis. To determine the antibiotic susceptibility of L. lactis, the minimum inhibitory concentration (MIC) test was conducted using five isolates (A1, A21, A65, A125 and A130) from Anguilla japonica and three isolates (KCTC2013, KCTC3769 and KCTC3899) from Korean Collection for Type Culture. Differences of MICs between A. japonica-derived isolates and KCTC isolates were observed in Tetracycline, Oxytetracycline, Doxycyclin and Erythromycin. MICs obtained for five L. lactis isolates from A. japonica were >64 μg/mL for Tetracycline, >256 μg/mL for Oxytetracycline, 16-32 μg/mL for Doxycyclin, and > 64μg/mL for Erythromycin. On the other hand, KCTC isolates showed the MICs of 0.25 μg/mL, 0.25 μg/mL, 0.12 μg/mL and 0.03-0.06 μg/mL for respective antibiotics. Furthermore, it was confirmed that genomic DNA and plasmid DNA isolated from A. japonica-derived L. lactis isolates have antibiotic resistance genes and transfer origin. These findings suggest that A. japonica-derived L. lactis isolates may have the potential to mediate the transfer of various antibiotic resistance genes. Therefore, it is advisable to use probiotics along with the feed or use them as postbiotics rather than dispersing them directly into the water in aquaculture system.
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