본문 바로가기
KERD
논문KCI 등재

극동산 뱀장어(Anguilla japonica)에서 Citrobacter freundii의 분리 및 특성 연구

Isolation and characterization of Citrobacter freundii from Japanese eel (Anguilla japonica)

1

조회

0

다운로드

0

인용

0

참고문헌

키워드

초록

Citrobacter freundii was the second most abundant bacterium isolated from domestically farmed Anguilla japonica. C. freundii is distributed across various environments as a facultative pathogen, and disease outbreaks have been reported in various aquatic and terrestrial animals. In this study, phylogenetic analysis, strain characteristics, antibiotic susceptibility, and pathogenicity to freshwater fish were analyzed. Phylogenetic analysis performed on 12 strains revealed the strains isolated from the same region belonged to the same clade. Three isolates (C. freundii AC52, C. freundii AC74, and C. freundii AG102) grew well in LA, BHIA, TSA, NA and MHA. They also showed growth under conditions of salinities of 0.5 to 7.5%, temperature of 10 to 40°C, and pH 5 to 10. As the results of antibiotic susceptibility test, 12 strains showed high resistance to ampicillin, amoxicillin, amoxicillin/clavulanic acid, cephalexin, clindamycin, and erythromycin. Moreover, they also had resist- ance genes such as tetD, floR, qnrB, strA-strB, and bla_TEM. When immunosuppressed by dex- amethasone injection, mortality was induced by C. freundii injection in Cyprinus carpio and Anguilla japonica. These results suggest that C. freundii can survive under various conditions of temperature, salinity, and drugs, spread through aquaculture wastewater or equipment, and can cause pathogenicity in freshwater fish.

Abstract

Citrobacter freundii was the second most abundant bacterium isolated from domestically farmed Anguilla japonica. C. freundii is distributed across various environments as a facultative pathogen, and disease outbreaks have been reported in various aquatic and terrestrial animals. In this study, phylogenetic analysis, strain characteristics, antibiotic susceptibility, and pathogenicity to freshwater fish were analyzed. Phylogenetic analysis performed on 12 strains revealed the strains isolated from the same region belonged to the same clade. Three isolates (C. freundii AC52, C. freundii AC74, and C. freundii AG102) grew well in LA, BHIA, TSA, NA and MHA. They also showed growth under conditions of salinities of 0.5 to 7.5%, temperature of 10 to 40°C, and pH 5 to 10. As the results of antibiotic susceptibility test, 12 strains showed high resistance to ampicillin, amoxicillin, amoxicillin/clavulanic acid, cephalexin, clindamycin, and erythromycin. Moreover, they also had resist- ance genes such as tetD, floR, qnrB, strA-strB, and bla_TEM. When immunosuppressed by dex- amethasone injection, mortality was induced by C. freundii injection in Cyprinus carpio and Anguilla japonica. These results suggest that C. freundii can survive under various conditions of temperature, salinity, and drugs, spread through aquaculture wastewater or equipment, and can cause pathogenicity in freshwater fish.

참고문헌 (30)

  1. Babu, L., "Optimization and evaluation of a multiplex PCR for simultaneous detection of prominent foodborne pathogens of Enterobacteriaceae", Annals of microbiology, 63, pp.1591~1599 (2013)[DOI]
  2. Behera, B. K., "Molecular identification and pathogenicity study of virulent Citrobacter freundii associated with mortality of farmed Labeo rohita (Hamilton 1822), in India", Aquaculture, 547, pp.737437~ (2022)[DOI]
  3. Cao HaiPeng, C. H., "Citrobacter freundii: a causative agent for tail rot disease in freshwater cultured Japanese eel Anguilla japonica", 68, pp.1271~ (2016)[DOI]
  4. Cui, J., "Toxicity effects of pesticides based on zebrafish (Danio rerio) models: Advances and perspectives", Chemosphere, pp.139825~ (2023)[DOI]
  5. Deng, D., "Pathogenicity of Plesiomonas shigelloides and Citrobacter freundii isolated from the endangered Chinese sturgeon (Acipenser sinensis)", Microbial Pathogenesis, 173, pp.105818~ (2022)[DOI]
  6. Stressmann, F. A., "Mining zebrafish microbiota reveals key community-level resistance against fish pathogen infection", The ISME journal, 15(3), pp.702~719 (2021)[DOI]
  7. Tao, R., "Detection of antibiotic resistance and tetracycline resistance genes in Enterobacteriaceae isolated from the Pearl rivers in South China", Environmental Pollution, 158(6), pp.2101~2109 (2010)[DOI]
  8. Fredriksson, N. J., "The choice of PCR primers has great impact on assessments of bacterial community diversity and dynamics in a wastewater treatment plant", PloS one, 8(10), pp.e7643~ (2013)[DOI]
  9. Hossain, S., "Multidrug resistant Aeromonas spp. isolated from zebrafish (Danio rerio): Antibiogram, antimicrobial resistance genes and class 1 integron gene cassettes", Letters in applied microbiology, 68(5), pp.370~377 (2019)[DOI]
  10. Jabeen, I., "A brief insight into Citrobacter species-a growing threat to public health", Frontiers in Antibiotics, 2, pp.1276982~ (2023)[DOI]
  11. Jeremić, S., "Citrobacter freundii as a cause of disease in fish", Acta veterinaria, 53(5-6), pp.399~410 (2003)[DOI]
  12. Jerez-Cepa, I., "What can we learn from glucocorticoid administration in fish? Effects of cortisol and dexamethasone on intermediary metabolism of gilthead seabream (Sparus aurata. L)", Comparative Biochemistry and Physiology Part A: Molecular Integrative Physiology, 231, pp.1~10 (2019)[DOI]
  13. Joh, S. J., "Bacterial pathogens and flora isolated from farm-cultured eels (Anguilla japonica)and their environmental waters in Korean eel farms", Veterinary microbiology, 163(1-2), pp.190~195 (2013)[DOI]
  14. Junior, G. B., "Citrobacter freundii infection in silver catfish (Rhamdia quelen): hematological and histological alterations", Microbial pathogenesis, 125, pp.276~280 (2018)[DOI]
  15. Jung, W. J., "Citrobacter tructae sp. nov. isolated from kidney of diseased rainbow trout (Oncorhynchus mykiss)", Microorganisms, 9(2), pp.275~ (2021)[DOI]
  16. Kim, S. W., "Carp edema virus and cyprinid herpesvirus-3 coinfection is associated with mass mortality of koi (Cyprinus carpio haematopterus) in the Republic of Korea", Pathogens, 9(3), pp.222~ (2020)[DOI]
  17. Larson, E., "Community factors in the development of antibiotic resistance", Annual Review of Public Health, 28(1), pp.435~447 (2007)[DOI]
  18. Li, M., "The damage of the crayfish (Procambarus Clarkii) digestive organs caused by Citrobacter Freundii is associated with the disturbance of intestinal microbiota and disruption of intestinal-liver Axis homeostasis", Frontiers in Cellular and Infection Microbiology, 12, pp.940576~ (2022)[DOI]
  19. Pan, L., "The novel pathogenic Citrobacter freundii (CFC202) isolated from diseased crucian carp (Carassius auratus)and its ghost vaccine as a new prophylactic strategy against infection", Aquaculture, 533, pp.736190~ (2021)[DOI]
  20. Price, L. B., "Colonizing opportunistic pathogens (COPs): The beasts in all of us", PLoS pathogens, 13(8), pp.e1006369~ (2017)[DOI]
  21. Sun, H. Y., "Outbreak of a novel disease associated with Citrobacter freundii infection in freshwater cultured stingray, Potamotrygon motoro", Aquaculture, 492, pp.35~39 (2018)[DOI]
  22. Uddin, M. N., "Optimum temperatures for the peak growth of some selected bacterial fish pathogens", Asian Fisheries Science, 21, pp.205~214 (2018)
  23. Vargas-Albores, F., "Therapeutic modulation of fish gut microbiota, a feasible strategy for aquaculture?", Aquaculture, 544, pp.737050~ (2021)[DOI]
  24. Verner-Jeffreys, D. W., "High prevalence of multidrug-tolerant bacteria and associated antimicrobial resistance genes isolated from ornamental fish and their carriage water", PloS one, 4(12), pp.e8388~ (2009)[DOI]
  25. Vestby, L. K., "Bacterial biofilm and its role in the pathogenesis of disease", Antibiotics, 9(2), pp.59~ (2020)[DOI]
  26. Wang, B., "Strategies to enhance tilapia immunity to improve their health in aquaculture", Reviews in Aquaculture, 15, pp.41~56 (2022)[DOI]
  27. Woo, S. J., "Establishment of epidemiological cut-off values and the distribution of resistance genes in Aeromonas hydrophila and Aeromonas veronii isolated from aquatic animals", Antibiotics, 11(3), pp.343~ (2022)[DOI]
  28. Zhao, C., "A novel disease (water bubble disease) of the giant freshwater prawn Macrobrachium rosenbergii caused by Citrobacter freundii: antibiotic treatment and effects on the antioxidant enzyme activity and immune responses", Antioxidants, 11(8), pp.1491~ (2022)[DOI]
  29. 김현우, "국내 양식 무지개송어 (Oncorhynchus mykiss) 에서 분리된 Photobacterium sp. YW2207의 특성", 한국어병학회지, 36(2), pp.251~261 (2023)[DOI]
  30. 이다원, "제주지역 양식 넙치(Paralichthys olivaceus)에서 분리한 어병세균 내 Erythromycin 내성 유전자 분석", 한국수산과학회지, 51(4), pp.397~403 (2018)[DOI]

관련 논문AI추천

이 간행물의 다른 논문 (19건)