알지네이트 코팅 Miamiensis avidus (Ciliophora: Scuticociliatida) 경구백신의 넙치(Paralichthys olivaceus)에 대한 효능평가
Efficacy of alginate microsphere oral vaccine against Miamiensis avidus (Ciliophora: Scuticociliatida) in olive flounder (Paralichthys olivaceus)
3
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
The efficacy of the alginate microsphere (Alginate MS) oral vaccine against Miamiensis avidus in olive flounder (Paralichthys olivaceus) was confirmed through challenge infections by both im- mersion and injection routes. In trial 1, the formalin-inactivated M. avidus coated with alginate, des- ignated as ‘IMa+Alginate MS’ group, and the IMa group were administered with vaccines mixed with feed, with a total antigen dose of 3.75 × 106 cells/fish. When challenged with immersion infection at five weeks post vaccination, the relative percent survival (RPS) in the IMa+Alginate MS group was 50% (immersed in 50% seawater) and 37.5% (immersed in 100% seawater). The group that received only IMa showed a low survival rate. In trial 2, the antigen was fed mixed with feed at a total dose of 2.38 × 106 cells/fish for 5 days. Two weeks after oral vaccination, fish were intra- peritoneally injected for infection. The RPS in the IMa+Alginate MS group was 30.8%, while the IMa-only group showed no vaccine efficacy. At five weeks post vaccination, when subjected to chal- lenge infection by immersion in 50% seawater, the IMa+Alginate MS group recorded a RPS of 42.9%, whereas the IMa group had a RPS of 14.3%. The results of this study indicate that coating M. avidus antigen with alginate can provide higher protection in olive flounder compared to administer- ing the antigen alone.
Abstract
The efficacy of the alginate microsphere (Alginate MS) oral vaccine against Miamiensis avidus in olive flounder (Paralichthys olivaceus) was confirmed through challenge infections by both im- mersion and injection routes. In trial 1, the formalin-inactivated M. avidus coated with alginate, des- ignated as ‘IMa+Alginate MS’ group, and the IMa group were administered with vaccines mixed with feed, with a total antigen dose of 3.75 × 106 cells/fish. When challenged with immersion infection at five weeks post vaccination, the relative percent survival (RPS) in the IMa+Alginate MS group was 50% (immersed in 50% seawater) and 37.5% (immersed in 100% seawater). The group that received only IMa showed a low survival rate. In trial 2, the antigen was fed mixed with feed at a total dose of 2.38 × 106 cells/fish for 5 days. Two weeks after oral vaccination, fish were intra- peritoneally injected for infection. The RPS in the IMa+Alginate MS group was 30.8%, while the IMa-only group showed no vaccine efficacy. At five weeks post vaccination, when subjected to chal- lenge infection by immersion in 50% seawater, the IMa+Alginate MS group recorded a RPS of 42.9%, whereas the IMa group had a RPS of 14.3%. The results of this study indicate that coating M. avidus antigen with alginate can provide higher protection in olive flounder compared to administer- ing the antigen alone.
참고문헌 (30)
- Amend, D. F., "Potency testing of fish vaccines. International symposium in fish biologics: serodiagnostics and vaccines", Developments in Biological Standardization, 49, pp.447~454 (1981)[DOI]
- Ballesteros, N. A., "An oral DNA vaccine against infectious haematopoietic necrosis virus (IHNV) encapsulated in alginate microspheres induces dose-dependent immune responses and significant protection in rainbow trout (Oncorrhynchus mykiss)", Fish and Shellfish Immunology, 45(2), pp.877~888 (2015)[DOI]
- Behera, T., "Alginate-chitosan-PLGA composite microspheres induce both innate and adaptive immune response through parenteral immunization in fish", Fish and Shellfish Immunology, 35(3), pp.785~791 (2013)[DOI]
- Fujiki, K., "Protective effect of sodium alginates against bacterial infection in common carp, Cyprinus carpio L", Journal of Fish Diseases, 17(4), pp.349~355 (1994)[DOI]
- Huttenhuis, H. B., "The effect of oral immuno-stimulation in juvenile carp (Cyprinus carpio L", Fish and Shellfish Immunology, 21(3), pp.261~271 (2006)[DOI]
- Iglesias, R., "Antiprotozoals effective in vitro against the scuticociliate fish pathogen Philasterides dicentrarchi", Diseases of Aquatic Organisms, 49(3), pp.191~197 (2002)[DOI]
- Jee, B. Y., "Morphology and biology of parasite responsible for scuticociliatosis of cultured olive flounder Paralichthys olivaceus", Diseases of Aquatic Organisms, 47(1), pp.49~55 (2001)[DOI]
- Jung, S. J., "Miamiensis avidus(Ciliophora : Scuticociliatida)causes systemic infection of olive flounder Paralichthys olivaceus and is a senior synonym of Philasterides dicentrarchi", Diseases of Aquatic Organisms, 73(3), pp.227~234 (2007)[DOI]
- Kang, Y. J., "Immunochemotherapy with doxycycline and CpG-ODN 1668 for treatment of scuticociliatosis in olive flounder (Paralichthys olivaceus).", Aquaculture, 435, pp.143~145 (2015)[DOI]
- Kim, S. M., "Occurrence of scuticociliatosis in olive flounder Paralichthys olivaceus by Philasterides dicentrarchi (Ciliophora: Scuticociliatida)", Diseases of Aquatic Organisms, 62(3), pp.233~238 (2004)[DOI]
- Kim, S. M., "Pseudocohnilembus persalinus (Ciliophora: Scuticociitida) is an additionalspecies causing scuticociliatosis in olive flounder Paralichthys olivaceus", Diseases of Aquatic Organisms, 62(3), pp.239~244 (2004)[DOI]
- Lamas, J., "Optimization of an inactivated vaccine against a scuticociliate parasite of turbot : effect of antigen, formalin and adjuvant concentration on antibody response and protection against the pathogen", Aquaculture, 278(1-4), pp.22~26 (2008)[DOI]
- Lee, E. H., "CpG-ODN increases resistance of olive flounder (Paralichthys olivaceus)against Philasterides dicentrarchi (Ciliophora: Scuticociliatia) infection", Fish and Shellfish Immunology, 26(1), pp.29~32 (2009)[DOI]
- Lee, K. Y., "Alginate : properties and biomedical applications", Progress in Polymer Science, 37(1), pp.106~126 (2012)[DOI]
- Lee, N. S., "Histopathological changes in fingerlings of Japanese flounder, Paralichthys olivaceus, with severe scuticociliatosis", Journal of Fish Pathology, 7(2), pp.151~160 (1994)
- Martins, S., "Insulin-loaded alginate microspheres for oral delivery–effect of polysaccharide reinforcement on physicochemical properties and release profile", Carbohydrate Polymers, 69(4), pp.725~731 (2007)[DOI]
- "National Fishery Products Quality Management Service" (2023)[URL]
- Paramá, A., "Philasterides dicentrarchi (Ciliophora, Scuticociliatida): experimental infection and possible routes of entry in farmed turbot (Scophthalmus maximus)", Aquaculture, 217, pp.73~80 (2003)[DOI]
- 박형준, "염분변화에 따른 넙치 (Paralichthys olivaceus)의 스트레스 반응", 한국어류학회지, 30(1), pp.1~8 (2018), 한국어류학회[DOI]
- Popov, A. L., "Ceria nanoparticles-decorated microcapsules as a smart drug delivery", protective system: protection of encapsulated P. pyralis luciferase, 2018, pp.10(17), 14367~14377
- Romalde, J. L., "Oral immunization using alginate microparticles as a useful strategy for booster vaccination against fish lactoccocosis", Aquaculture, 236, pp.119~129 (2004)[DOI]
- Rombout, J. W., "Immunization of carp(Cyprinus carpio)with a Vibrio anguillarum bacterin : indications for a common mucosal immune system", Developmental and Comparative Immunology, 10(3), pp.341~351 (1986)[DOI]
- Russell-Jones, G. J., "Oral vaccine delivery", Journal of Controlled Release, 65, pp.49~54 (2000)[DOI]
- Sanmartín, M. L., "Vaccination of turbot, Psetta maxima (L.), against the protozoan parasite Philasterides dicentrarchi: effects on antibody production and protection", Journal of Fish Diseases, 31(2), pp.135~140 (2008)[DOI]
- Shin, S. M., "Formulation of chitosan microsphere-based oral vaccine against the scuticociliate parasite Miamiensis avidus: Evaluation of its protective immune potency in olive flounder (Paralichthys olivaceus)", Fish and Shellfish Immunology, 142, pp.109159~ (2023)[DOI]
- Somamoto, T., "Mucosal delivery of fish vaccines : Local and systemic immunity following mucosal immunisations", Fish and Shellfish Immunology, 99, pp.199~207 (2020)[DOI]
- Song, J. Y., "Pathogenicity of Miamiensis avidus (syn. Philasterides dicentrarchi), Pseudocohnilembus persalinus, Pseudocohnilembus hargisi and Uronema marinum (Ciliophora, Scuticociliatida)", Diseases of Aquatic Organisms, 83(2), pp.133~143 (2009)[DOI]
- Takagishi, N., "Effect of hyposalinity on the infection and pathogenicity of Miamiensis avidus causing scuticociliatosis in olive flounder Paralichthys olivaceus", Diseases of Aquatic Organisms, 86(2), pp.175~179 (2009)[DOI]
- Tian, J. Y., "Formation and oral administration of alginate microspheres loaded with pDNA coding for lymphocystis disease virus(LCDV)to Japanese flounder", Fish and Shellfish Immunology, 24(5), pp.592~599 (2008)[DOI]
- Xu, C., "An oral microencapsulated vaccine loaded by sodium alginate effectively enhances protection against GCRV infection in grass carp(Ctenopharyngodon idella)", Frontiers in Immunology, 13, pp.848958~ (2022)[DOI]