국내 양식 무지개송어(Oncorhynchus mykiss)에서 분리된 Photobacterium sp. YW2207의 특성
Characterization of Photobacterium sp. YW2207 isolated from rainbow trout (Oncorhynchus mykiss) raised in a fresh water farm in South Korea
0
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Photobacterium sp. YW2207 was isolated from rainbow trout raised in a fish farm located in Yeongwol-gun, Gangwon Province, South Korea. Based on 16S rRNA sequence analysis and phyloge- netic analysis, it was confirmed that Photobacterium sp. YW2207 showed 100% similarity with Photobacterium piscicola and Photobacterium phosphoreum, and 94.6% similarity with P. damselae subsp. damselae. Biochemical analysis revealed that Photobacterium sp. YW2207 is a Gram-negative, motile bacterium with a cell size of 1.5~3×3~5 μm. The bacteria were cultured on nutrient agar, brain heart infusion agar, Muller-Hinton agar, tryptic soy agar, and thiosulfate citrate bile sucrose agar with NaCl concentrations ranging from 0 to 2.5%. The API50CHE and API20E tests indicated lower utilization capabilities compared to the P. damselae strains provided in the API database. Furthermore, unlike most Photobacterium species, Photobacterium sp. YW2207 presented negative for catalase test. Results from the flow cytometric measurement indicated that Photobacterium sp.YW2207 exhibited a more diverse distribution of cell sizes and had larger cell sizes compared with P. damselae subsp. damselae. Minimum inhibitory concentration tests showed that Photobacterium sp. YW2207 had low susceptibility to β-Lactam and aminoglycoside antibiotics, while having high susceptibility to tetracycline, doxycycline, and quinolone antibiotics. Pathogenicity on rainbow trout revealed that an immersion of 1×105 CFU/ml did not cause mortality or clinical symptoms.
Abstract
Photobacterium sp. YW2207 was isolated from rainbow trout raised in a fish farm located in Yeongwol-gun, Gangwon Province, South Korea. Based on 16S rRNA sequence analysis and phyloge- netic analysis, it was confirmed that Photobacterium sp. YW2207 showed 100% similarity with Photobacterium piscicola and Photobacterium phosphoreum, and 94.6% similarity with P. damselae subsp. damselae. Biochemical analysis revealed that Photobacterium sp. YW2207 is a Gram-negative, motile bacterium with a cell size of 1.5~3×3~5 μm. The bacteria were cultured on nutrient agar, brain heart infusion agar, Muller-Hinton agar, tryptic soy agar, and thiosulfate citrate bile sucrose agar with NaCl concentrations ranging from 0 to 2.5%. The API50CHE and API20E tests indicated lower utilization capabilities compared to the P. damselae strains provided in the API database. Furthermore, unlike most Photobacterium species, Photobacterium sp. YW2207 presented negative for catalase test. Results from the flow cytometric measurement indicated that Photobacterium sp.YW2207 exhibited a more diverse distribution of cell sizes and had larger cell sizes compared with P. damselae subsp. damselae. Minimum inhibitory concentration tests showed that Photobacterium sp. YW2207 had low susceptibility to β-Lactam and aminoglycoside antibiotics, while having high susceptibility to tetracycline, doxycycline, and quinolone antibiotics. Pathogenicity on rainbow trout revealed that an immersion of 1×105 CFU/ml did not cause mortality or clinical symptoms.
참고문헌 (19)
- Austin, B., "Bacterial Fish Pathogens", pp.327~339 (2012), Springer
- Bao, X. C., "Bioluminescence Contributes to the Adaptation of Deep-Sea Bacterium Photobacterium phosphor-eum ANT-2200 to High Hydrostatic Pressure", Mi-croorganisms, 11(6), pp.1362~ (2023)[DOI]
- Betts, G., "Food Spoilage Microorganisms, Woodhead Publishing Series in Food Science", pp.668~693 (2006)
- CIHEAM, "Diagnostic Manual for the main pathogens in European seabass and Gilthead seabream aquaculture", CIHEAM, 83~96 (2020)
- Clinical and Laboratory Standards Institute, "M45 Methods for Antimicrobial Dilution and Disk Suscepti- bility Testing of Infrequently Isolated or Fastidious Bacteria", ~ (2018)
- Deep, K., "Photobacterium pan- uliri sp. nov., an Alkalitolerant Marine Bacterium Isolated from Eggs of Spiny Lobster, Panulirus penicillatus from Andaman Sea", Current Microbiology, 69(5), pp.660~668 (2014)[DOI]
- Dunstan, J., "Evaporation-driven convective flows in suspen- sions of non-motile bacteria", Physical Review Fluids, 3(12), pp.123102~ (2018)[DOI]
- European Committee On Antimicrobial Susceptibility Testing, "Broth microdilution – EUCAST reading guide v 4.0", EUCAST, ~ (2022)
- Flodgaard, L. R., "Nonbiolumines-cent strains of Photobacterium phosphoreum pro-duce the cell-to-cell communication signal N-(3-Hydroxyoctanoyl)homoserine lactone", Applied and environmental microbiology, 71(4), pp.2113~2120 (2005)[DOI]
- Fouz, F., "Survival of fish-virulent strains of Photobacte- rium damselae subsp. damselae in seawater under starvation conditions", FEMS Microbiology Letters, 168(2), pp.181~186 (1998)[DOI]
- Marian, J. F., "Photobacterium piscicola sp. nov., isolated from marine fish and spoiled packed cod", Systematic and Applied Microbiology, 37(5), pp.329~335 (2014)[DOI]
- Terceti, M. S., "Molecular epi- demiology of Photobacterium damselae subsp. dam- selae outbreaks in marine rainbow trout farms re- veals extensive horizontal gene transfer and high ge- netic diversity", Frontiers in microbiology, 9, pp.2155~ (2018)[DOI]
- Paul, S. I., "Identification of marine sponge-as-sociated bacteria of the Saint Martin's island of the Bay of Bengal emphasizing on the prevention of motile Aeromonas septicemia in Labeo rohita", Aquaculture, 545, pp.737156~ (2021)[DOI]
- Waters, P., "Salt, pH and temperature de-pendencies of growth and bioluminescence of three species of luminous bacteria analysis on gradient plates", Journal of General Microbiology, 131, pp.2865~2869 (1985)[DOI]
- 국립수산과학원, "2022 수산용의약품 제품 요약 해설집" (2022), 해양수산부 국립수산과학원
- 국립수산과학원, "무지개송어 양식 표준 매뉴얼", 해양수산부 국립수산과학원, ~ (2017)
- 국립수산과학원, "무지개송어 합동이동병원운영", 국립수산과학원 보도자료, ~ (2023)
- 권문경, "넙치, Paralichthys olivaceus에서 병원성 Photobacterium damselae subsp. damselae의 분리", 한국어병학회지, 18(3), pp.205~214 (2005), 한국어병학회
- 이은수, "독성 물질 측정을 위한Photobacterium phosphoreum의 고정화에 관한 연구", 식량자원개발연구소 논문집, 19, pp.48~56 (1998)