Polylactide-co-glycolic acid (PLGA)에 캡슐화된 Edwardsiella tarda 포르말린 사멸백신의 이스라엘 잉어(Cyprinus carpio)에 있어서의 효능
Efficacy of polylactide-co-glycolic acid (PLGA) microparticle encapsulating Edwardsiella tarda formalin killed cell (FKC) vaccine in Israeli carp (Cyprinus carpio)
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This study evaluated the effectiveness of polylactide-co-glycolic acid (PLGA) as a vaccine delivery vehicle in fish. Israeli carp were immunized with a formalin-inactivated Edwardsiella tarda vaccine (PLGA-FKC) encapsulated in PLGA microparticles using the W/O/W emulsion method, administered both orally and via intraperitoneal (I.P.) injection. Immune responses were analyzed, including antibody titers, cytokine expression, and antibacterial activity. Results showed that PLGA-FKC maintained anti- genicity longer than FKC, with elevated antibody titers and immune responses at weeks 8 and 10. The PLGA-FKC vaccine demonstrated enhanced pathogen resistance, with survival rates of 60% for oral and 70% for i.p. administration. As results, the study confirms that both oral and i.p administration of PLGA-FKC vaccines in adult carp elicit immune enhancement and pathogen resistance. These results support the need for further studies on PLGA-encapsulated oral vaccines targeting various pathogenic microorganisms, including bacteria like E. tarda and viruses.
Abstract
This study evaluated the effectiveness of polylactide-co-glycolic acid (PLGA) as a vaccine delivery vehicle in fish. Israeli carp were immunized with a formalin-inactivated Edwardsiella tarda vaccine (PLGA-FKC) encapsulated in PLGA microparticles using the W/O/W emulsion method, administered both orally and via intraperitoneal (I.P.) injection. Immune responses were analyzed, including antibody titers, cytokine expression, and antibacterial activity. Results showed that PLGA-FKC maintained anti- genicity longer than FKC, with elevated antibody titers and immune responses at weeks 8 and 10. The PLGA-FKC vaccine demonstrated enhanced pathogen resistance, with survival rates of 60% for oral and 70% for i.p. administration. As results, the study confirms that both oral and i.p administration of PLGA-FKC vaccines in adult carp elicit immune enhancement and pathogen resistance. These results support the need for further studies on PLGA-encapsulated oral vaccines targeting various pathogenic microorganisms, including bacteria like E. tarda and viruses.
참고문헌 (29)
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