넙치(Paralichthys olivaceus)에서 분리된 Vibrio tapetis에 대한 주사 백신의 개발 및 평가
Development and evaluation of an injectable vaccine against Vibrio tapetis isolated from olive flounder (Paralichthys olivaceus)
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Vibrio tapetis, originally known as the causative agent of brown ring disease in clams, has recently emerged as an opportunistic pathogen of olive flounder (Paralichthys olivaceus), with recurrent de- tections during low-temperature seasons. To address this threat, we developed a formalin-killed cell (FKC) vaccine and two adjuvanted formulations using squalene–aluminum hydroxide (FKC-SQ) or Montanide™ ISA 760 VG (FKC-Seppic). Vaccinated olive flounder were assessed for antibody re- sponses, histopathological changes, and protective efficacy. FKC-Seppic induced the highest agglutinat- ing antibody titers, whereas ELISA showed uniformly elevated antibody levels across all vaccine groups. Histopathology revealed severe granulomatous inflammation and organ adhesions in FKC-Seppic fish, while FKC-SQ caused only mild localized inflammation and FKC minimal tissue reactions. After challenge with V. tapetis at 15℃, survival reached 83.33% (RPS 66.67%) in both adjuvanted groups and 72.22% (RPS 44.44%) in the FKC group, compared with 50% in control. Although differences were not statistically significant, vaccinated fish consistently exhibited improved survival. These findings indicate that FKC-based vaccines provide partial protection against V. tapetis, and that the squalene–alum formulation offers a more favorable balance of efficacy and safety than Montanide ISA 760 VG, supporting its potential application in olive flounder aquaculture.
Abstract
Vibrio tapetis, originally known as the causative agent of brown ring disease in clams, has recently emerged as an opportunistic pathogen of olive flounder (Paralichthys olivaceus), with recurrent de- tections during low-temperature seasons. To address this threat, we developed a formalin-killed cell (FKC) vaccine and two adjuvanted formulations using squalene–aluminum hydroxide (FKC-SQ) or Montanide™ ISA 760 VG (FKC-Seppic). Vaccinated olive flounder were assessed for antibody re- sponses, histopathological changes, and protective efficacy. FKC-Seppic induced the highest agglutinat- ing antibody titers, whereas ELISA showed uniformly elevated antibody levels across all vaccine groups. Histopathology revealed severe granulomatous inflammation and organ adhesions in FKC-Seppic fish, while FKC-SQ caused only mild localized inflammation and FKC minimal tissue reactions. After challenge with V. tapetis at 15℃, survival reached 83.33% (RPS 66.67%) in both adjuvanted groups and 72.22% (RPS 44.44%) in the FKC group, compared with 50% in control. Although differences were not statistically significant, vaccinated fish consistently exhibited improved survival. These findings indicate that FKC-based vaccines provide partial protection against V. tapetis, and that the squalene–alum formulation offers a more favorable balance of efficacy and safety than Montanide ISA 760 VG, supporting its potential application in olive flounder aquaculture.