EHP (Enterocytozoon hepatopenaei)에 오염된 사육기구에 대한 UV-C와 차아염소산칼슘의 소독 효과
The disinfection effect of UV-C and calcium hypochlorite to shrimp farm instruments contaminated with EHP (Enterocytozoon hepatopenaei)
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In this study, nylon mash and silicone tube mainly used as shrimp farm equipment were con-taminated with Enterocytozoon hepatopenaei (EHP) which is the cause of Hepatopancreatic micro-sporidiosis (HPM), and were treated with calcium hypochlorite or UV-C disinfection methods for EHP eradication. As a result, similar with the control group (not disinfected), EHP was detected on the nested PCR until the 10 days in the UV-C single treated group. On the other hand, EHP was not detected from 7 days in calcium hypochlorite single treated group (total concentration 200 ppm as available chlorine), and combination of calcium hypochlorite and UV-C treated group revealed no detection of EHP from 3 days. It is appropriate that treated with UV-C and calcium hypochlorite for 3 days or single treated with calcium hypochlorite for 7 days to eradicate EHP on contaminated instrument used in shrimp farms. In contrast, disinfection effect of only using UV-C is very low.
Abstract
In this study, nylon mash and silicone tube mainly used as shrimp farm equipment were con-taminated with Enterocytozoon hepatopenaei (EHP) which is the cause of Hepatopancreatic micro-sporidiosis (HPM), and were treated with calcium hypochlorite or UV-C disinfection methods for EHP eradication. As a result, similar with the control group (not disinfected), EHP was detected on the nested PCR until the 10 days in the UV-C single treated group. On the other hand, EHP was not detected from 7 days in calcium hypochlorite single treated group (total concentration 200 ppm as available chlorine), and combination of calcium hypochlorite and UV-C treated group revealed no detection of EHP from 3 days. It is appropriate that treated with UV-C and calcium hypochlorite for 3 days or single treated with calcium hypochlorite for 7 days to eradicate EHP on contaminated instrument used in shrimp farms. In contrast, disinfection effect of only using UV-C is very low.
참고문헌 (39)
- Aldama-Cano, D. J., "Bioassay for spore polar tube extrusion of shrimp Enterocytozoon hepatopenaei(EHP)", Aquaculture, 490, pp.156~161 (2018)[DOI]
- Bratvold, D., "Disinfection, microbial community establishment and shrimp production in a prototype biosecure pond", Journal of the world aquaculture society, 30, pp.422~432 (1999)[DOI]
- Cali, A., "The Microsporidia: pathology in man and occurrence in nature", SE Asian J. Trop. Med. Public Health, 35(Suppl 1), pp.58~64 (2004)
- Chaijarasphong, T., "The shrimp microsporidian Enterocytozoon hepatopenaei(EHP) : Biology, pathology, diagnostics and control", Journal of invertebrate pathology, 186, pp.107458~ (2021)[DOI]
- Chang, P. S., "The effect of ultraviolet irradiation, heat, pH, ozone, salinity and chemical disinfectants on the infectivity of white spot syndrome baculovirus", Aquaculture, 166, pp.1~17 (1998)[DOI]
- Delabbio, J. L., "Fish disease and biosecurity : attitudes, beliefs, and perceptions of managers and owners of commercial finfish recirculating facilities in the United States and Canada", Journal of Aquatic Animal Health, 17, pp.153~159 (2005)[DOI]
- Jang, G. I., "First report of Enterocytozoon hepatopenaei infection in giant freshwater prawn(Macrobrachium rosenbergii de Man)cultured in the Republic of Korea", Animals, 12, pp.3149~ (2022)[DOI]
- Jaroenlak, P., "A nested PCR assay to avoid false positive detection of the microsporidian Enterocytozoon hepatopenaei (EHP) in environmental samples in shrimp farms", PloS one, 11, pp.e0166320~ (2016)[DOI]
- Jena, K., "Calcium hypochlorite causes annihilation of bio-molecules of nosema spores purified Antheraea Mylitta", Int. J. Adv. Res, 5(4), pp.1958~1965 (2017)[DOI]
- Kasai, H., "Disinfection of water for aquaculture", Fisheries science, 68, pp.821~824 (2002)[DOI]
- Keeling, P. J., "Microsporidia : biology and evolution of highly reduced intracellular parasites", Annual Reviews in Microbiology, 56, pp.93~116 (2002)[DOI]
- Kim, B. S., "First report of Enterocytozoon hepatopenaei infection in Pacific whiteleg shrimp(Litopenaeus vannamei)cultured in Korea", Animals, 11, pp.3150~ (2021)[DOI]
- Kumar, T. S., "Effect of Enterocytozoon hepatopenaei(EHP)infection on physiology, metabolism, immunity, and growth of Penaeus vannamei", Aquaculture, 553, pp.738105~ (2022)[DOI]
- Kwon, M. G., "Epidemiological survey of infectious myonecrosis in farmed whiteleg shrimps(Litopenaeus vannamei)in Korea", The Journal of the Korean Society for Fisheries and Marine Sciences Education, 31(1), pp.94~99 (2019)[DOI]
- Leblanc, B. D., "Efficacy of calcium hypochlorite as a disinfectant against the shrimp virus Baculovirus penaei", Journal of Aquatic Animal Health, 3, pp.141~145 (1991)[DOI]
- Leal, J. F., "Use of formalin in intensive aquaculture : properties, application and effects on fish and water quality", Reviews in Aquaculture, 10(2), pp.281~295 (2018)[DOI]
- Li, H., "A review of influencing factors on a recirculating aquaculture system : Environmental conditions, feeding strategies, and disinfection methods", Journal of the world aquaculture society, 54, pp.566~602 (2023)[DOI]
- 2.0.CO;2,
- Lightner, D. V., "Biosecurity in shrimp farming : Pathogen exclusion through use of SPF stock and routine surveillance", Journal of the world aquaculture society, 36, pp.229~248 (2005)[DOI]
- Limsuwan, C., "Efficacy of calcium hypochlorite on the prevalence of microsporidiosis(Thelohania)in pond-reared Litopenaeus vannamei", Kasetsart J Nat Sci, 42, pp.282~288 (2008)
- Lotz, J., "Viruses, biosecurity and specific pathogen-free stocks in shrimp aquaculture", World journal of microbiology and biotechnology, 13, pp.405~413 (1997)[DOI]
- Mascarenhas dos Santos, A. C., "The Rad9-Rad1-Hus1 DNA Repair clamp is found in microsporidia", Genome Biology and Evolution, 14(4), pp.evac053~ (2022)[DOI]
- Mohammed, A. N., "Resistance of bacterial pathogens to calcium hypochlorite disinfectant and evaluation of the usability of treated filter paper impregnated with nanosilver composite for drinking water purification", Journal of Global Antimicrobial Resistance, 16, pp.28~35 (2019)[DOI]
- Park, J. H., "Safety and protective effect of a disinfectant(STEL water)for white spot syndrome viral infection in shrimp", Diseases of aquatic organisms, 60, pp.253~257 (2004)[DOI]
- Patil, P. K., "Economic loss due to diseases in Indian shrimp farming with special reference to Enterocytozoon hepatopenaei(EHP)and white spot syndrome virus(WSSV)", Aquaculture, 533, pp.736231~ (2021)[DOI]
- Pattarayingsakul, W., "Shrimp microsporidian EHP spores in culture water lose activity in 10 days or can be inactivated quickly with chlorine", Aquaculture, 548, pp.737665~ (2022)[DOI]
- Schuur, A. M., "Evaluation of biosecurity applications for intensive shrimp farming", Aquacultural Engineering, 28, pp.3~20 (2003)[DOI]
- Subash, P., "In vivo and in silico investigations on the efficacy of albendazole against Enterocytozoon hepatopenaei(EHP)infecting Penaeus vannamei", Aquaculture, 575, pp.739801~ (2023)[DOI]
- Teitge, F., "Water disinfection by ozonation has advantages over UV irradiation in a brackish water recirculation aquaculture system for Pacific white shrimp(Litopenaeus vannamei)", Journal of fish diseases, 43, pp.259~1285 (2020)[DOI]
- Venkobachar, C., "Mechanism of disinfection: effect of chlorine on cell membrane functions", Water Research, 11(8), pp.727~729 (1977)
- Wang, L., "Wholegenome sequencing and comparative genomic analysis of a pathogenic Enterocytozoon hepatopenaei strain isolated from Litopenaeus vannamei", Aquaculture International, 31(1), pp.523~546 (2023)[DOI]
- Watanabe, K. I., "Disinfection of equipments for aquaculture and fertilized eggs by ozonated seawater", Fish Pathology, 33, pp.145~146 (1998)[DOI]
- Weber, R., "Manual of clinical microbiology", pp.2209~2219 (2015)
- Williams, B. A., "Microsporidia:pathogens of opportunity", pp.245~260 (2014)
- Wiredu Boakye, D., "Decay of the glycolytic pathway and adaptation to intranuclear parasitism within Enterocytozoonidae microsporidia", Environmental Microbiology, 19(5), pp.2077~2089 (2017)[DOI]
- Yanong, R. P., "Biosecurity in aquaculture, Part 1 : An overview", Southern regional aquaculture center, 4707, pp.1~16 (2012)
- Yuanlae, S., "Shrimp injection with dsRNA targeting the microsporidian EHP polar tube protein reduces internal and external parasite amplification", Scientific Reports, 14, pp.4830~ (2024)[DOI]
- Zhang, Y., "Laser Tweezers Raman Spectroscopy (LTRS) to Detect Effects of Chlorine Dioxide on Individual Nosema bombycis Spores", Applied Spectroscopy, 73(7), pp.774~780 (2019)[DOI]
- 이영옥, "소독제제의 효과적 사용과 국내현황", 대한수의사회지, 20, pp.528~537 (1984)
