Disinfectant effect of monopersulfate (MPS) compound to white spot syndrome virus (WSSV) of shrimp
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This study investigated the disinfection effect of monopersulfate (MPS) compound against white spot syndrome virus (WSSV) by bioassay using kuruma shrimp (Marsupenaeus japonicas). A WSSV stock was prepared with muscle homogenate from WSSV-infected whiteleg shrimp (Penaeus vannamei) and its lethal dose 50% endpoint (LD50) and infectious dose 50% endpoint (SID50) were respectively determined as 10-5.63 and 10-6.79 in bioassay using kuruma shrimp, followed by PCR assays. The disinfective effect of MPS compound (1.2 ppm, 2.4 ppm, 4.8 ppm) was performed by bioassay using about 10-fold higher dilution (10-4) of WSSV homogenate. The compound resulted in WSSV inactivation by a concentration-dependent manner. In addition, 4.8 ppm of MPS completely prohibited WSSV infection. To our knowledge, this study is the first report about the usefulness of MPS as a disinfectant to WSSV.
Abstract
This study investigated the disinfection effect of monopersulfate (MPS) compound against white spot syndrome virus (WSSV) by bioassay using kuruma shrimp (Marsupenaeus japonicas). A WSSV stock was prepared with muscle homogenate from WSSV-infected whiteleg shrimp (Penaeus vannamei) and its lethal dose 50% endpoint (LD50) and infectious dose 50% endpoint (SID50) were respectively determined as 10-5.63 and 10-6.79 in bioassay using kuruma shrimp, followed by PCR assays. The disinfective effect of MPS compound (1.2 ppm, 2.4 ppm, 4.8 ppm) was performed by bioassay using about 10-fold higher dilution (10-4) of WSSV homogenate. The compound resulted in WSSV inactivation by a concentration-dependent manner. In addition, 4.8 ppm of MPS completely prohibited WSSV infection. To our knowledge, this study is the first report about the usefulness of MPS as a disinfectant to WSSV.
참고문헌 (12)
- Alday-Sanz, V, "Specific pathogen free (SPF), Specific pathogen resistant (SPR) and Specific pathogen tolerant (SPT) as part of the biosecurity strategy for whiteleg shrimp (Penaeus vannamei)", Asian Fish Science, 31, pp.112~120 (2018)
- Bir, J., "A critical review on white spot syndrome virus (WSSV): A potential threat to shrimp farming in Bangladesh and some Asian countries", International Journal of Microbiology and Mycology, 6, pp.39~48 (2017)
- Escobedo-Bonilla, C., "In vivo titration of white spot syndrome virus(WSSV)in specific pathogen-free Litopenaeus vannamei by intramuscular and oral routes", Diseases of Aquatic Organisms, 66, pp.163~170 (2005)
- Masák, J., "Pseudomonas biofilms: possibilities of their control", FEMS Microbiology Ecology, 89, pp.1~14 (2014)
- 민정란, "모노퍼설페이트 화합물의 Vibrio harveyi에 대한 살균력 및 횐다리새우(Litopenaeus vannamei)에서의 독성", 한국수산과학회지, 48(5), pp.661~667 (2015), 한국수산과학회
- Oseko, N., "Examination for viral inactivation of WSSV(White Spot Syndrome Virus)isolated in Malaysia using black tiger prawn(Penaeus monodon)", Japan Agricultural Research Quarterly, 40, pp.93~97 (2006)
- Park, J., "An infectious viral disease of penaeid shrimp newly found in Korea", Diseases of Aquatic Organisms, 34, pp.71~75 (1998)
- Park, J. H., "Safety and protective effect of a disinfectant(STEL water)for white spot syndrome viral infection in shrimp", Diseases of Aquatic Organisms, 60, pp.253~257 (2004)
- Sonthipet, S., "Bactericidal and virucidal efficacies of potassium monopersulfate and its application for inactivating avian influenza virus on virus-spiked clothes", The Journal of Veterinary Medical Science, 80, pp.568~573 (2018)
- Shukur, S. A., "Water quality measuring in swimming pools by oxidation reduction potential method", International Journal of Research, 3, pp.553~576 (2016)
- Withyachumnarnkul, B, "Results from black tiger shrimp Penaeus monodon culture ponds stocked with postlarvae PCR-positive or negative for white-spot syndrome virus(WSSV)", Diseases of Aquatic Organisms, 39, pp.21~27 (1999)
- Zhu, F., "A new method for quantifying white spot syndrome virus: Experimental challenge dose using TaqMan real-time PCR assay", Journal of Virological Methods, 184, pp.121~124 (2012)