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Sex ratio and conception rates of fresh / vitrified embryos at different developmental stages by ovum pick up in Hanwoo cows

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주제분류축산학

초록

Embryo transfer plays a crucial role in enhancing the breeding value of livestock; it has been applied in Hanwoo cattle, which is a popular breed for beef production in Korea. Both in vivo- derived (IVD) and in vitro-produced (IVP) embryos are used for this purpose; however, IVP embryos have been preferred recently owing to advancements in ovum pick-up (OPU) technology and genomic selection. Despite technological advancements, comprehensive data on large-scale OPU/IVEP/embryo transfer in Hanwoo cows are lacking. In this study, 16 elite Hanwoo donor cows were selected on the basis of specific criteria. Oocytes were retrieved from 241 cows using OPU. The collected cumulus-oocyte complexes (COCs) were matured, fertilized, and cultured in vitro to produce transferable embryos. Embryos were classified according to their developmental stage and then transferred to 675 recipient cows. A total of 3,317 COCs were collected, with an average of 13.76 COCs per cow. The number of transferable embryos produced per cow was 3.7. Hanwoo OPU-derived IVP embryos exhibited a higher production yield than the global average, indicating a stable IVEP environment. Both fresh and frozen IVP embryos yielded similar conception rates; hence, the use of vitrified-thawed embryos in transfer plans feasible. However, frozen-thawed embryos at Stage 7 had a lower conception rate than those at earlier stages. There was no significant difference between the conception rates of sexually mature heifers and postpartum cows used as recipients. The male-to-female offspring ratio increased as the developmental stage progressed. Seasonal effects on conception rates were not observed; however, higher abortion rates and a higher proportion of male offspring were observed during winter. This study provides valuable data for the Korean embryo transfer industry, enabling more strategic growth of the domestic Hanwoo embryo industry.

Abstract

Embryo transfer plays a crucial role in enhancing the breeding value of livestock; it has been applied in Hanwoo cattle, which is a popular breed for beef production in Korea. Both in vivo- derived (IVD) and in vitro-produced (IVP) embryos are used for this purpose; however, IVP embryos have been preferred recently owing to advancements in ovum pick-up (OPU) technology and genomic selection. Despite technological advancements, comprehensive data on large-scale OPU/IVEP/embryo transfer in Hanwoo cows are lacking. In this study, 16 elite Hanwoo donor cows were selected on the basis of specific criteria. Oocytes were retrieved from 241 cows using OPU. The collected cumulus-oocyte complexes (COCs) were matured, fertilized, and cultured in vitro to produce transferable embryos. Embryos were classified according to their developmental stage and then transferred to 675 recipient cows. A total of 3,317 COCs were collected, with an average of 13.76 COCs per cow. The number of transferable embryos produced per cow was 3.7. Hanwoo OPU-derived IVP embryos exhibited a higher production yield than the global average, indicating a stable IVEP environment. Both fresh and frozen IVP embryos yielded similar conception rates; hence, the use of vitrified-thawed embryos in transfer plans feasible. However, frozen-thawed embryos at Stage 7 had a lower conception rate than those at earlier stages. There was no significant difference between the conception rates of sexually mature heifers and postpartum cows used as recipients. The male-to-female offspring ratio increased as the developmental stage progressed. Seasonal effects on conception rates were not observed; however, higher abortion rates and a higher proportion of male offspring were observed during winter. This study provides valuable data for the Korean embryo transfer industry, enabling more strategic growth of the domestic Hanwoo embryo industry.

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Improved pregnancy rate and sex ratio in fresh/frozen in vivo derived embryo transfer of Hanwoo (Bos taurus coreanae) cows이상훈, 이준구, Islam M. Saadeldin, Jihyun Park, Jong Ki Cho, Seonggyu Bang, Wonyou Lee한국축산학회 · 한국축산학회지2023 · 제65권 제4호한우 공란우 및 생체내 난자 회수(ovum pick-up) 조건이 체외수 정란의 발달에 미치는 효과박용수, 이준구, 정기화, Dong yep Oh, Jun Ho Kong한국동물위생학회 · 한국동물위생학회지2021 · 제44권 제4호고능력 한우의 체내수정란 생산효율과 수정란이식김현, 고응규, 김성우, 김영신, 김재환, 도윤정, 박해금, 성환후, 염규태, 조상래동물생명과학연구소 · 동물자원연구2013 · 제24권 제1호Effects of culture media conditions on production of eggs fertilized in vitro of embryos derived from ovary of high grade HanwooByoung Boo Seo, Jun Young Lee, Yun Gil Jung한국축산학회 · 한국축산학회지2016 · 제58권 제3호한우 수정란의 Biopsy 후 배발달율과 동결-융해후 생존성 및 성비김현종, 손동수, 조상래, 조창연, 진현주, 최선호, 최창용한국축산학회 · 한국축산학회지2007 · 제49권 제2호Conception rate according to the size and location of corpus luteum and coexistent follicle before embryo transfer in Hanwoo권우성, 박진연, 유대중, 임수근, Dae-HyunKim, Jae Jung Ha, Junkoo Yi, Zae Young RYOO한국축산학회 · 한국축산학회지2024 · 제66권 제6호

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