소 로타바이러스 특이적 단일도메인 항체의 경구투여를 통한 송아지에서의 소 로타바이러스 감염 억제 효과
Effect of Inhibition against Bovine Rotavirus Infection in Calves by Oral Administration of Bovine Rotavirus-Specific Single Variable Domain of Heavy-chain Antibodies
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본 연구는 소 로타바이러스는 송아지에게 발생 할 수 있는 흔한 소화기성 질병 중 하나로, 심각한 설사를 일으킬 수 있다. 본 연구는 단일 도메인 항체, single variable domain of heavy chain of HCAb(VHH)의 경구투여가 송아지에게 있어 로타 바이러스로 인한 설사 예방 및 치료에 미치는 효과를 조사하기 위해 수행되었다. 송아지 30마리를 5개 시험군(NC, PC, A, B, C 그룹)으로 나누어 실험을 진행했다. NC 그룹을 제외한 시험군은 입식 후 4일차에 송아지 로타 바이러스를 공격접종 받았으며, A, B, C 그룹은 농도를 달리한 1%, 2.5%, 7.5%의 BR 커버를 대용유에 혼합하여 급여받았다. 모든 시험군은 총 10일 간 체중, 임상증상, 혈중 면역항체, 분변 내 바이러스 검출 등의 변화를 관찰하였다. 실험 결과, VHH 첨가 받은 A, B, C 그룹에서 PC 그룹에 비해 높은 증체율이 관찰되었으며 로타 바이러스로 인한 임상증상이 완화되었다. 또한, 분변 내 로타 바이러스 검출률이 감소한 것으로 보아 VHH는 송아지의 로타 바이러스성 설사 예방과 치료에 유용한 대안으로 제시될 수 있다.
Abstract
Bovine rotavirus (BRV) is one of the common gastrointestinal diseases that can occur in calves, causing severe diarrhea. This study was conducted to investigate the effects of oral administration of single-domain antibodies, single variable domain of heavy chain of HCAb (VHH), on the prevention and treatment of rotavirus-induced diarrhea in calves. Thirty calves were divided into five experimental groups (negative control, positive control, group A, group B and group C). Except for the negative control group, the experimental groups were inoculated with BRV on the 4th day after birth. The VHH supplementation groups were fed with a substitute milk containing different concentrations of VHH (1%, 2.5% and 7.5%). Changes in body weight, clinical symptoms, serum antibodies, and virus detection in feces were observed for a total of 10 days in each experimental group. The results showed a higher survival rate in the VHH supplementation groups compared to the positive control group. Clinical symptoms caused by rotavirus were alleviated in the VHH supplementation groups. The detection rate of rotavirus in feces also decreased. Gross findings indicated that the severity of disease in the VHH supplementation groups was milder than that in the positive control group. Therefore, VHH could be proposed as a useful alternative for the prevention and treatment of rotavirus-induced diarrhea in calves.
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