신속 진단 킷트를 활용한 한우 송아지의 설사증 원인체 검사
Use of rapid diagnostic kit for the diagnosis of Korean native calf diarrhea
2
조회
0
다운로드
4
인용
0
참고문헌
키워드
초록
Calf diarrhea is a disease experienced by almost all of calves after birth and is one of the representative causes of damage to farmers due to mass mortality and of economic losses to them by inhibiting normal growth. In this study, we conducted quick detection of etiologic agents of diarrhea by using a rapid diagnostic kit to multiply diagnose antigens of five etiologic agents of calf diarrhea (rotavirus, coronavi-rus, Escherichia coli, Cryptosporidium, Giardia) in Hanwoo (Korean native cattle) calves. When the positive antigen proportion of the calf diarrheal feces for each farm was analyzed, rotavirus, coronavirus, Escherichia coli, Cryptosporidium, and Giardia showed antigen positive rates of 0∼67%, 0∼20%, 0∼60%, 0∼20%, and 0∼67%, respectively. With regard to the antigen positive rate by age in days after birth, 1-week-old calves showed the antigen positive rate of 20% in rotavirus and 20% in Giardia, and 2-week-old calves showed that of 50% in rotavirus. In addition, 4-week-old calves showed the antigen positive rate of 10% in rotavirus, 10% in coronavirus, 10% in Escherichia coli, and 30% in Giardia, and 8-week-old calves showed the antigen positive rate of 17% in coronavirus, 50% in Escherichia coli, 17% in Cryptosporidium, and 33% in Giardia. Based on the results of this study, the etiologic agents of diarrhea in Hanwoo calves for each farm are widely distributed. Although younger than 2-week-old calves were strongly positive for rotavirus, older than 4-week-old calves were highly positive for Giardia and Escherichia coli. In conclusion, we considered that a rapid diagnostic kit is an effective method for quick detection of etiologic agents and would be helpful for cattle farmers and veterinarians to select appropriate therapeutic method.
Abstract
Calf diarrhea is a disease experienced by almost all of calves after birth and is one of the representative causes of damage to farmers due to mass mortality and of economic losses to them by inhibiting normal growth. In this study, we conducted quick detection of etiologic agents of diarrhea by using a rapid diagnostic kit to multiply diagnose antigens of five etiologic agents of calf diarrhea (rotavirus, coronavi-rus, Escherichia coli, Cryptosporidium, Giardia) in Hanwoo (Korean native cattle) calves. When the positive antigen proportion of the calf diarrheal feces for each farm was analyzed, rotavirus, coronavirus, Escherichia coli, Cryptosporidium, and Giardia showed antigen positive rates of 0∼67%, 0∼20%, 0∼60%, 0∼20%, and 0∼67%, respectively. With regard to the antigen positive rate by age in days after birth, 1-week-old calves showed the antigen positive rate of 20% in rotavirus and 20% in Giardia, and 2-week-old calves showed that of 50% in rotavirus. In addition, 4-week-old calves showed the antigen positive rate of 10% in rotavirus, 10% in coronavirus, 10% in Escherichia coli, and 30% in Giardia, and 8-week-old calves showed the antigen positive rate of 17% in coronavirus, 50% in Escherichia coli, 17% in Cryptosporidium, and 33% in Giardia. Based on the results of this study, the etiologic agents of diarrhea in Hanwoo calves for each farm are widely distributed. Although younger than 2-week-old calves were strongly positive for rotavirus, older than 4-week-old calves were highly positive for Giardia and Escherichia coli. In conclusion, we considered that a rapid diagnostic kit is an effective method for quick detection of etiologic agents and would be helpful for cattle farmers and veterinarians to select appropriate therapeutic method.
참고문헌 (32)
- Abdel-Moein KA, "The zoonotic potential of Giardia intestinalis assemblage E in rural settings", Parasitol Res, 115, pp.3197~3202 (2016)
- Acres SD, "Studies on rotaviral antidody in bovine serum and lacteal secretions using radioimmunoassay", J Am Vet Med Assoc, 173, pp.555~559 (1978)
- Ahn JM, "Detection of bovine coronavirus in fecal samples by reverse transcriptase polymerase chain reaction", Korean J Vet Serv, 22, pp.239~245 (1999)
- Ahn JM, "Studies on protective efficacy of Escherichia coli vaccines", Korean J Vet Serv, 15, pp.26~31 (1992)
- Ahn SC, "A ssudy of calf diarrhea caused by rotavirus", Korean J Vet Serv, pp.238~ (1997)
- 백강현, "송아지에서 발생한 중증 크립토스포리듐 증 증례", 대한수의학회지, 54(4), pp.257~260 (2014), 대한수의학회[DOI]
- Bjorkman C, "Cryptosporidium parvum and Giardia intestinalis in calf diarrhea in Sweden", Acta Ve. Scnad, 44, pp.145~152 (2003)
- Bouzid M, "Cryptosporidium pathogenicity and virulence", Clin Microbiol Rev, 26, pp.115~134 (2013)
- Chai JY, "An epidermiological survey of Cryptosporidium parvum infection in randomly selected inhabitants of Seoul and Chollanam-do", Korean J Parasitol, 34, pp.113~119 (1996)
- 조영권, "Magnetic Resonance Imaging Feature of Bacterial Meningitis in a Neonate Hanwoo Calf", 한국임상수의학회지, 26(6), pp.650~654 (2009), 한국임상수의학회
- 전승기, "한우 송아지의 설사 분변에서 Escherichia coli(K99), Clostridium perfringens와 Cryptosporidium parvum의 검출", 한국임상수의학회지, 24(4), pp.588~592 (2007), 한국임상수의학회
- Harp JA, "Strategies for the control of Cryptosporidium parvum infection in calves", J Dairy Sci, 81, pp.289~294 (1998)
- Kang MI, "Survey on Korean-native calves diseases and mortality", Korean J Vet Serv, 24, pp.223~241 (2001)
- Kim D, "Etiology and clinical aspects of diarrhea of Korean native calves during the suckling period", Korean J Vet Res, 30, pp.255~260 (1990)
- Kim HY, "Domestic outbreaks of calves’ diarrhea in Korea", Korean J Buiat, 21, pp.61~81 (2016)
- Kim JM, "Studies on development of Escherichia coli subunit vaccine against calf diarrhea: Ι. Distribution of enterotoxigenic Escherichia coli and purification of K99 and F41 pilus antigens", Korean J Vet Res, 26, pp.97~102 (1986)
- Kim JS, "Study on treatment of neonatal calf diarrhea", Korean J Vet Serv, pp.238~ (1997)
- Kim KS, "Bovine coronavirus diarrhea precautionary!", J Korean Vet Med Assoc, 35, pp.658~661 (1999)
- 김남수, "개의 Giardia 감염에 따른 albendazole, fenbendazole, metronidazole의 치료효과", 한국임상수의학회지, 22(3), pp.239~243 (2005), 한국임상수의학회
- 김윤희, "낙동강 중류수계에서 Cryptosporidium과 Giardia의 분포 특성", 대한환경공학회지, 28(8), pp.843~851 (2006), 대한환경공학회
- Lim JS, "Detection of viral-like particles in feces of Korean indigenous calves with diarrhea by negative staining", Korean J Vet Serv, 22, pp.43~52 (1999)
- Lyoo YS, "Calf diarrhea caused by rotavirus", J Korean Vet Med Assoc, 24, pp.657~670 (1988)
- Panciera RJ, "Cryptosporidial infections in a calf", Vet Pathol, 8, pp.479~484 (1971)
- Park BK, "Viral diarrhea of calf", J Korean Vet Med Assoc, 20, pp.147~153 (1984)
- Reynolds DJ, "Microbiology of calf diarrhea in southern Britain", Vet Rec, 119, pp.34~39 (1986)
- Saif LJ, "Winter dysentery in dairy herds: electron microscopic and serological evidence for and association with coronavirus infection", Vet Rec, 128, pp.447~449 (1991)
- Snodgrass DR, "Passive immunity in calf diarrhea; Vaccination with K99 antigen of enterotoxigenic Escherichia coli and Rotavirus", Inf Immunol, 37, pp.586~ (1982)
- Speicher JA, "Factors associated with calf mortality in Michigan dairy herds", J Am Vet Med Assoc, 162, pp.463~466 (1973)
- Takahash, E, "Epizootic diarrhea of adult cattle associated with a coronavirus-like agent", Vet Microbiol, 5, pp.151~154 (1980)
- Wegayehu T, "Multilocus genotyping of Giardia duodenalis isolates from calves in Oromia Special Zone, Central Ethiopia", Infect Genet Evol, 43, pp.281~288 (2016)
- Yang HJ, "Prevalence of Cryptosporidium sp among calves and pigs in Jeonbuk province", Korean J Vet Serv, 24, pp.133~138 (2001)
- 육심용, "충남 서산태안지역에서 사육중인 송아지의 코로나바이러스 감염률 조사", 한국가축위생학회지, 32(3), pp.209~214 (2009), 한국동물위생학회