랫드 발바닥 염증부위에서 관찰된 zinc 함유 비만세포의 미세구조: 조직화학적 염색을 중심으로
Ultrastructures of zinc-containing mast cells found in the rat hindpaw after an inflammatory stimuli: Zinc selenium autometallography
2
조회
0
다운로드
0
인용
0
참고문헌
초록
본 연구에서는 흰쥐 발바닥에 complete Freund's adjuvant(CFA)를 투여한 후 염증부위에서 관찰되는 비만세포의 분포양상 및 미세구조를 zinc selenium autometallography(AMGZnSe)로 염색한 후 광학 및 전자현미경으로 관찰하였다. 광학현미경으로 관찰된 CFA투여에 의한 염증성 반응은 피하층에 국한되어 있었으며, zinc함유 비만세포는 비만세포는 주로 피하조직의 혈관 주변에 분포하고 있었다. AMG 염색에 의한 흑갈색을 띠는 과립이 세포질을 채우고 있었으며, 많은 비만세포의 경우 세포막이 파괴되어 과립이 주위조직으로 퍼져 관찰되었다. 전자현미경으로 관찰된 zinc함유 비만세포는 불규칙한 외형을 띠었으며, 표면에는 작은 돌기들이 관찰되었다. AMG 염색에 따른 은입자 (silver grains)가 세포질내 과립에 국한되었으며, 높은 전자 밀도의 동질성 기질로 구성되어 있었으며, 경계막으로 싸여 있었다. 세포질내 세포소기관 중에서 특히 골지 복합체가 잘 발달 되어 있었으며, 형질내세망과 사립체는 비교적 적게 가지고 있었다. 이들 세포의 핵은 비교적 작고 둥글고, 세포의 중심부에 위치하였으며, 이질염색질로 이루어져 있었다.
Abstract
Mast cells (MCs) are granulated cells that play a pivotal role in allergic reaction and inflammation. The granules of mast cells are known to be rich in zinc (Zn). Male Sprague-Dawley rats were used. We injected 200 ㎕ of complete Freund's adjuvant (CFA) subcutaneously in the dorsal aspect of one hindpaw. Finally, zinc selenium autometallography (AMG) was done by Danscher's method. The present study showed the ultrastructures of zinc-containing mast cells found in inflammatory area following an complete freund's adjuvant (CFA) inoculation into the rat hindpaw.At light microscopic level, mast cells were round or oval, at average 12 μm in diameter, with many filopodia extending from the cell surface. Because the rather small and spherical nucleus was centrally placed; it was frequently obscured by the cytoplasmic granules, it sometimes could not be seen. Mast cells were distributed chiefly in the vicinity of small blood vessels. In most preparation many mast cells were ruptured and their granules escaped into the surrounding tissue.In electron micrographs, The secretory granules were at average 0.5 μm in diameter and were limited by a membrane. The cell surface contained numerous microvilli and folds. Their interior was heterogenous in appearance. The nucleus was surrounded by large numbers of prominent vesicels and a well developed Golgi apparatus, but scant endoplasmic reticulum.
참고문헌 (22)
- "Alteration of ionic zinc distribution in rat spinal ganglion by inflammatory pain stimulus: Autometallography", 38, pp.561~566 (2005)
- "Do paneth cells regulate the zinc body burden?.", 30, pp.357~365 (2000)
- "The influence of zinc-deprivation on the mast cell population the bone marrow and other tissues", 108, pp.1315~1321 (1978)
- "Histochemical demonstration of Heavy metals:A revised version of the sulphide silver method suitable for both light and electron microscopy", pp.1~16
- "Electron microscopic demostration of metals in rat mast cell:A cytochemical study based on an improved sulphide silver method", pp.293~300
- "Effects of copper iron and zinc on edema formation induced by phospholipase A₂", pp.325~327 (1992)
- ":Heavy metals in rat mast cell granules", pp.588~
- "Labile zinc and zinc transporter ZnT4 in mast cell granules role in regulation of caspase activation and NF-kappaB translocation", 172, pp.7750~7760 (2004)
- "The mast cell", 48, pp.40~ (1992)
- "The differential contribution of tumour necrosis factor to thermal and mechanical hyperalgesia during chronic inflammation" (2005)
- "Zinc-enriched (ZEN) terminals in mouse spinal cord: immunohistochemistry and autometallography", 870, pp.163~169 (2000)
- "Do paneath cells regulate the zinc body burden?.", 30, pp.357~365 (2000)
- "Zinc is required for FcepsilonRI-mediated mast cell activation", 177, pp.1296~1235 (2006)
- "Modulation by Zn2+ of GABA responses in bipolar cells of the mouse retina", 17, pp.273~281 (2000)
- "Zinc alleviates thermal hyperalgesia due to partial nerve injury", 10, pp.1619~1623 (1999)
- "Physiological concentrations of zinc inhibit the release of histamine from human basophils and lung mast cells", 18, pp.103~106 (1986)
- "Mast cells", 77 (1997)
- "Biochemical and physiological indicators of mineral status in animals", pp.1702~11 (1987), cobalt and zinc
- ":Pharmacological characterization of the effects of 5-hydroxytryptamine and different prostaglandins on peripheral sensory neurons in vitro", pp.~15
- "Ultrastructure of mast cells in the hyperplastic buccal mucosa of the zinc-deficient rat", 14, pp.375~82 (1985)
- "Unilateral spinal nerve ligation leads to an asymmetrical distribution of mast cells in the thalamus of female but not male mice", 114, pp.131~140 (2005)
- "The biochemical basis of zinc physiology", pp.79~118