Histochemically-reactive Zinc in the Rat Dorsal Root Ganglion (DRG) Neurons: Zinc Selenium Autometallography
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The present study was designed to demonstrate ionic zinc in the rat DRG by means of zinc selenium autometallography (ZnSeAMG). Ganglion cells varied in size from 15 to 100 μm. The smaller neurons were strongly stained with AMG, whereas the larger cells were weakly stained. Each large ganglion cell was surrounded by perineuronal satellite cells, showing apparent AMG staining. We demonstrated for the first time the existence of zinc-containing satellite cells in the rodent DRG. Using electron microscopy, fine AMG grains were observed scattered in the somata of the DRG neurons, especially small cells. However, much lower concentrations of the AMG grains occupied in the large cells, and these were mostly localized in lysosome-like organelles. These results indicate that zinc may be involved in sensory transmission in the DRG level.
Abstract
The present study was designed to demonstrate ionic zinc in the rat DRG by means of zinc selenium autometallography (ZnSeAMG). Ganglion cells varied in size from 15 to 100 μm. The smaller neurons were strongly stained with AMG, whereas the larger cells were weakly stained. Each large ganglion cell was surrounded by perineuronal satellite cells, showing apparent AMG staining. We demonstrated for the first time the existence of zinc-containing satellite cells in the rodent DRG. Using electron microscopy, fine AMG grains were observed scattered in the somata of the DRG neurons, especially small cells. However, much lower concentrations of the AMG grains occupied in the large cells, and these were mostly localized in lysosome-like organelles. These results indicate that zinc may be involved in sensory transmission in the DRG level.
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