멜라닌 세포에서 홍차 열수추출물의 멜라닌 합성 저해능과 작용기전
Inhibitory Efficacy of Black Tea Water Extract on Melanogenesis in Melan-a Cells and Its Action Mechanisms
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홍차 열수추출물의 멜라닌 합성 저해능과 작용기전을 알아보기 위해 in vitro 실험으로 항산화능을 확인한 후, melan-a 세포를사용하여 멜라닌 합성 저해능, tyrosinase 활성 저해능 및 발현저해능 등에 대한 실험을 실시하여 다음과 같은 결과를 얻었다. (1) 항산화능 실험에서 홍차 열수추출물의 총 폴리페놀 및 플라보노이드 함량은 각각 102, 87 mg/g으로 양성대조군인 알부틴(25, 11 mg/g)보다 높았고, 전자공여능 역시 800 μg/mL에서 82%로 알부틴(48%)보다 높았다. (2) Melan-a 세포 실험에서 홍차 열수추출물은 수지상돌기의 발달과 멜라닌 생합성을 효과적으로저해하였고, 멜라닌 합성 저해능과 tyrosinase 활성 저해능이 알부틴보다 유의하게 (p⁄0.001) 높았다. (3) Tyrosinase 단백질 발현 저해능 실험에서 알부틴은 발현에 유의한 영향을 미치지 않은 반면, 홍차 열수추출물은 발현을 유의하게 (p⁄0.001) 저해하였다. (4) 유전자 발현 저해능 실험에서 알부틴과 홍차 열수추출물 모두 tyrosinase, TRP-1과 TRP-2의 mRNA 발현에 영향을 미치지 않았다. 본 연구를 통하여, 홍차 열수추출물은 우수한 항산화능과 함께 멜라닌 합성 저해능을 확인하였으며, 이는 tyrosinase 의 생합성을 저해시키기 보다는 tyrosinase 단백질 발현량을 줄이고 tyrosinase 활성을 감소시킴으로써 멜라닌 합성을 저해시키는 것으로 사료된다.
Abstract
To evaluate the whitening effect of Camellia sinensis water extract (CSWE), CSWE was treated to melan-a cells. Total polyphenol contents and flavonoid contents of CSWE were 102 mg/g and 87 mg/g, respectively. The electron-donating ability of CSWE revealed a dose-dependent response, showing the excellent ability of 82% at 800 μg/mL, and which was higher than the arbutin (48%). The CSWE significantly (p⁄0.00l) suppressed the melanin synthesis and the development of melanocyte dendrites was inhibited in a dose-dependent manner. The CSWE significantly (p⁄0.00l) inhibited both intra-cellular and cell-extracted tyrosinase activities. And inhibitory efficacies of CSWE on both melanin synthesis and tyrosinase activity were significantly (p⁄0.00l) higher than the arbutin. The tyrosinase protein expression was not influenced by arbutin treatment. However, CSWE treatment significantly (p⁄0.00l) reduced it. Both arbutin and CSWE treatment did not influence on mRNA expressions of tyrosinase, tyrosinase-related protein-1 and tyrosinase related protein-2.
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