돼지감자 에탄올 추출물의 피부미백 효능과 작용기전
Skin-Whitening Efficacy of Helianthus tuberosus Ethanol Extract and Its Mechanism of Action
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본 연구는 피부미백제로서의 기능성 입증이 미흡한 돼지감자 에탄올 추출물을 사용하여 항산화능을 알아보고, melan-a 세포에서 멜라닌 합성 저해능과 tyrosinase 유전자 및 단백질 발현에 미치는 영향을 알아봄으로써 천연 미백제로서의 활용 가능성을 살펴보았다. 돼지감자 에탄올 추출물의 총 폴리페놀 및 총 플라보노이드 함량은 각각 71.6, 25.0 mg/g이었고, 전자공여능은 1,000 μg/mL 에서 28.9%로 우수한 항산화능을 나타냈다. Melan-a 세포에 대 한 돼지감자 에탄올 추출물의 최대허용농도는 200 μg/mL 이상 으로 확인되어 세포독성이 미약하였다. 100 μg/mL 농도에서 돼 지감자 에탄올 추출물은 멜라닌 생성을 11.6% (p<0.05), 세포 내 tyrosinase 활성을 21.1% (p<0.05), tyrosinase mRNA 발현과 단백질 발현을 각각 11.7%, 14.4% 유의하게 (p<0.05) 감소시켰 다. 이상의 실험 결과를 종합해 보면, 돼지감자 에탄올 추출물은 tyrosinase 유전자의 전사를 방해하여 tyrosinase 활성을 저해시 킴으로써 미백효능을 나타냄을 확인하였다. 이들 실험 결과를 근거로 볼 때, 돼지감자 에탄올 추출물은 피부미백용 기능성 화 장품 천연소재로서 활용 가능성이 있을 것으로 생각된다
Abstract
The objectives of this study was to evaluate the skin-whitening efficacy of Helianthus tuberosus ethanol extract (HTEE) and its mechanism of action in melan-a cells. The total contents of polyphenolics and flavonoids in HTEE were 71.6 and 25.0 mg/g, respectively. At HTEE concentration of 1,000 μg/mL, its electron-donating ability was 28.9%. An MTT assay showed that the maximum permissible HTEE concentration for treatment of melan-a cells was over 200 μg/mL. In comparison with the vehicle control, treatment with HTEE at 100 μg/mL significantly decreased melanin contents (by 11.6%), intracellular tyrosinase activity (by 21.1%), tyrosinase messenger RNA expression (by 11.7%) and its protein level (by 14.4%, all p<0.05). We conclude that HTEE has a considerable skin-whitening efficacy in melan-a cells and this inhibition is exerted through inhibition of tyrosinase transcription. Thus, HTEE can be expected to be useful as a cosmetic skin-whitening agent.
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