찔레 추출물의 B16 세포 멜라닌 형성 억제
Melanogenesis Inhibition Effect of Rosa multiflora extracts in B16 Melanoma Cells
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본 연구는 찔레 에탄올 추출물의 세포 활성 및 티로시나 아제활성 저해를 분석하였다. 찔레 추출물의 세포활성을 분석한 결과 찔레 잎과 뿌리 추출물의 경우에는 추출물을 처리하지 않은 대조군과 유사한 결과를 관찰하였다. 모든 농도에서 세포 활성이 나타나지 않아 티로시나아제 활성 저해 효과를 분석한 결과 찔레 잎과 뿌리추출물은 상대적 으로 고농도(200 μg/ml)에서 약 30~32% 정도를 저해하 는 효과를 나타냈다. 또한 B16 세포에서 티로시나아제활성 저해 효과를 분석한 결과 상대적으로 고농도(200 μg/ml) 에서 양성대조군인 알부틴보다 20~25% 정도 낮은 저해 효과를 나타냈다. 멜라닌 함량 저해 효과를 분석한 결과 찔 레 잎과 뿌리 추출물의 경우 티로시나아제의 저해 효과와 유사하게 농도 의존적인 저해효과를 나타내는 것을 관찰하 였다. RT-PCR 방법으로 분석한 결과, 추출물 처리군들은 정상대조군과 비교하여 티로시나아제 mRNA 수준이 유의 적으로 차이가 나지 않았으며, 또한 농도 의존적인 변화도 없었다.
Abstract
In the present study, the effect of ethanol extracts of leaf and root of Rosa multiflora on the proliferation of B16 cells, tyrosinase activity and melanin synthesis were investigated. 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay demonstrated that the cell viability upon treatment with Rosa multiflora extract (0-200 μg/ml) was similar to that of untreated control. Treatment with leaf or root extracts (200 μg/ml) decreased the in vitro tyrosinase activity to about 65% of that in untreated control. Similarly, the intracellular tyrosinase activity of B16 cells decreased by the two in a concentration-dependent manner. Furthermore, the melanin synthesis of B16 cells decreased by the two extracts in a concentration dependent manner. However, the extracts did not change the level of tyrosinase mRNA, as determined by RT-PCR. These results demonstrated that the Rosa multiflora extracts inhibit the tyrosinase dependent melanin biosynthesis, and therefore, are candidates for skin-whitening agents.
참고문헌 (21)
- Chien, C.C., "Effects on tyrosinase activity by the extracts of Ganoderma lucidum and related mushrooms", Mycopathologia, 166(2), pp.117~120 (2008)
- Dedeoglu, N., "Differential in vitro inhibition of polyphenoloxidase from a wild edible mushroom Lactarius salmonicolor", J Enzyme Inhib Med Chem., 2, pp.1~ (2008)
- Eisenman, H., "Cryptococcus neoformans laccase catalyses melanin synthesis from both D-and L-DOPA.Microbiology.153:3954-62", Microbiology, 153, pp.3954~3962 (2007)
- Hosoi,J., "Regulation of melanin synthesis of B16 mouse melanoma cells by 1 alpha, 25-dihydroxyvitamin D3 and retinoic acid", Cancer Res., 45(4), pp.1474~1478 (1985)
- 황은영, "백삼과 홍삼의 페놀성 성분 함량 및 멜라닌 생성억제효과", Journal of Ginseng Research, 30(2), pp.82~87 (2006), 고려인삼학회
- Hyun, S.K., "Inhibitory Effects of Kurarinol, Kuraridinol, and Trifolirhizin from Sophora flavescens on Tyrosinase and Melanin Synthesis", Biol. Pharm. Bull., 31(1), pp.154~158 (2008)
- Jang, K.H., "Depigmentation activity of many herb", Research Institute of Korean Medicone., 13(2), pp.289~302 (2004)
- Kim J.H., "Inhibitory effects of calycosin isolated from the root of Astragalus membranaceus on melanin biosynthesis", Biol Pharm Bull., 32(2), pp.264~268 (2009)
- 김정수, "효모에서 추출한 Melanoston의 색소침착억제 및 미백효과", 대한피부과학회지, 44(4), pp.391~398 (2006), 대한피부과학회
- Kim, Y.M., "Oxyresveratrol and hydroxystilbene compounds. Inhibitory effect on tyrosinase and mechanism of action", J Biol Chem., 277(18), pp.16340~16344 (2002)
- Lee,K.K., "The effects of areca catechu L extract on anti-inflammation and anti-melanogenesis", Int J Cosmet Sci., 21(4), pp.275~284 (1999)
- 이송주, "배나무 절지 추출물의 총 페놀함량, 전자공여능 및 Tyrosinase 활성 저해 효과", 원예과학기술지, 24(3), pp.338~341 (2006), 한국원예학회
- Lim,S.H., "Change of biophysical properties of the skin caused by ultraviolet radiation-induced photodamage in Koreans", Skin Res Technol., 14(1), pp.93~102 (2008)
- McEwan,M., "Inhibition of melanization in human melanoma cells by a serotonin uptake inhibitor", J Invest Dermatol., 89(1), pp.82~86 (1987)
- Meyskens,F.J., "Characterization of the effects of different retinoids on the growth and differentiation of a human melanoma cell line and selected subclones", Cancer Res., 40(7), pp.2194~2196 (1980)
- Santos,C., "Melanin-based high-throughput screen for L-tyrosine production in Escherichia coli", Appl Environ Microbiol., 74(4), pp.1190~1197 (2008)
- Seto,T., "Purgative activity and principals of the fruits of Rosa multiflora and R. wichuraiana", Chem Pharm Bull(Tokyo)., 40(8), pp.2080~2082 (1992)
- Shin, N.H., "Oxyresveratrol as the potent inhibitor on dopa oxidase activity of mushroom tyrosinase", Biochem Biophys Res Commun., 243(3), pp.801~803 (1998)
- T. Kuriki, "Inhibitory Effects of a-Arbutin on Melanin Synthesis in Cultured Human Melanoma Cells and a Three-Dimensional Human Skin Model", Biol. Pharm. Bull., 27(4), pp.510~514 (2004)
- Tomita,Y., "Inactivation of tyrosinase by dopa", J Invest Dermatol., 75(5), pp.379~382 (1980)
- Ai-Hua Wen, "Formulation of Liposome for Topical Delivery of Arbutin", Archives of Pharmacal Research, 29(12), pp.1187~1192 (2006), 대한약학회