홍바리, Epinephelus fasciatus 정자의 미세구조
Ultrastructure of Blacktip Grouper, Epinephelus fasciatus Spermatozoa
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홍바리, Epinephelus fasciatus 정자의 미세구조적 특징을 주사형 및 투과형 전자현미경으로 관찰하였다. 홍바리 정자는 구형의두부와 cytoplasmic sleeve와 고리형 cytoplasmic canal 입구 등으로 구성된 중편부 그리고 lateral fin을 갖는 1개의 편모로 구성된 미부로 구별된다. 정자의 핵은 구형이며, 첨체 구조물은 존재하지 않으나 이질 염색질이 과립상 덩어리 형태로 조밀하게 분포하였다. Proximal centriole과 distal centriole은 각각 수레바퀴형의 “9+0”의 축사구조를 보이며 서로 수직으로 osmophilic filaments 연결되어 있고, 핵막과도 연결되어 있었다. 중편부는 구형의 8~9개의 미토콘드리아를 포함하며, 빈공간인 cytoplasmic canal과 편모 원형질막에는 7~8개의 구형 입자를 갖는 necklace 가 존재하였다. 미부는 1개의 편모를 갖으며 전형적인 “9+2”의축사구조를 보였고, 편모에는 다양한 형태의 lateral fins을 관찰할 수 있었다. 홍바리 정자의 미세구조적 특징은 바리과 어류의정자와 상이하여 계통분류학적 동정의 형질이 될 수 있을 뿐만아니라 정자 세포소기관의 기능적 및 생리학적 연구에 이용될수 있다고 판단된다.
Abstract
Ultrastructural characteristics of blacktip grouper, Epinephelus fasciatus spermatozoa were investigated using transmission and scanning electron microscopy. The spermatozoa of E. fasciatus consisted of a spherical head part, a midpiece with cytoplasmic canal entrance and a flagellum with lateral fins. Internal ultrastructurally, the nucleus contains high electron dense chromatin having granular particles and has no acrosome. The centriolar complex lies outside of the nuclear fossa and it is connected by the osmophilic filaments. Also the osmophilic filaments connect between the centriolar complex and the nuclear membrane. The midpiece contains eight to nine spherical mitochondria, cytoplasmic canal and necklaces. The flagellum has a typical 9+2 axonemal structure. The lateral fins contain vesicles and a typical 9+2 axonemal structure. Consequently this study contributes to comparative grouper spermatology and provide useful systematic taxonomic characters.
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