무당개구리 (Bombina orientalis) 정자의 미세구조
Sperm Ultrastructure of Bombina orientalis
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초록
본 연구는 무당개구리과 (Discoglossidae)의 무당개구리(Bombina orientalis) 정자의 형태적 특징을 광학현미경과 주사 및 투과 전자현미경으로 관찰하였다. 정자두부는전 후방의 끝이 뾰족하고 다소 유연성을 갖는 초생달 혹은 버들 잎 모양의 특이한 구조를 취하고 있었다. 핵은전후방이 가늘고, 중앙 부분은 굵은 원뿔형이었다. 염색질은 완전하게 농축되어 있지 않고, 마치 널빤지를 겹쳐놓은 듯한 지붕모양과 같은 형태로 불규칙하게 배열되어져 있었다. 핵 내에는 불규칙한 형태의 nuclear lacunae들이 흩어져 있었다. 정자경부와 중편부는 발달 되어 있지않았다. 편모는 9± 2 구조의 axoneme, axial rod 그리고undulating membrane을 가지고 있었다. 미토콘드리아들은핵의 상단부를 둘러싸고 있는 protoplasmic bead내에만국한되어 존재하고 있었다. 특히, 본 연구에서 핵의 전방부에서 중앙 부위로 주행하는 nuclear rod는 섬유성 다발로 구성되었으며, 마치 사람의 속눈썹 모양과 같은 형태를 취하며, 핵의 2/3까지 뻗쳐져 있었다.
Abstract
This study described the spermatozoa of the discoglossidae Bombina orientalisusing light, scanning andtransmission electron microscopes. Sperm head possess a crescent or leaf shape, with a moderately flexiblehead, and with a sharp anterior and posterior tips. The nucleus are a thick cone shaped in the widest middle partof nucleus, and a slender anterior and posterior of nuclear tips. The chromatin is not completely compact, butNo neck and middle piece were developed. The flagellum is composed of 9± 2 axoneme, axial rod andundulating membrane. The mitochondria were distributed only in cytoplasmic membrane around the nucleus.In particular, the nuclear rod contains bundles of fibers, the rod penetrating from anterior portion to the middleof the nucleus, is extended roughly two-thirds of the nucleus such as eyelashes shaped.
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