홍바리, Epinephelus fasciatus의 인공수정 전ㆍ후 난문의 미세구조적 변화
Ultrastructural Variations on the Micropyle of Blacktip Grouper, Epinephelus Fasciatus before and after Artificial Fertilization
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홍바리, Epinephelus fasciatus 난의 인공수정 전ㆍ후 난문의미세구조적 특징을 주사형 전자현미경으로 관찰하였다. 홍바리난문(6.6±0.41 μm)은 원통형의 분화구형으로 동물극에 1개가존재하였다. 난문의 vestibular ridge는 시계방향성을 갖는 6~7개의 나선형의 내벽구조로 구성되었다. 난문의 내벽구조물인 나선형의 vestibular ridge는 인공수정 후 부풀어 올라와 돔(dome)형의 수정구로 분화 형성되었다. 홍바리 난은 부상란으로 난막표면에는 pore 가장자리에 고리형의 nodule과 pore 사이에 돌기형의 nodule이 관찰되었으나, 부착사와 유사한 보조 구조물은 관찰할 수 없었다. 난문 주변부에는 동심원상으로 크기가 다른 pore (0.15~0.55 μm)들이 개구된 형태로 혼재하였고, 수정 후 돔(dome)형의 수정구가 분화 형성되면서 pore들이 닫히는 현상을보였다. 홍바리 난문의 미세구조적 특징은 바리과 어종간에 형태학적으로 상이하여, 바리과 어류의 계통분류학의 형질로 이용될수 있다고 판단된다.
Abstract
The micropyles on the prefertilized and artificial fertilized eggs of Epinephelus fasciatus were investigated using scanning electron microscope (SEM). The micropyles (6.6±0.41 μm) of E. fasciatus eggs were found in the animal pole and theirs shape were observed as a flat crateriform of cylindrical shape. The micropylar vestibule arranged by 6~7 thickened spiral annuli on the ridge and contributed to differentiate and form fertilization cone for blocking to polyspermy by presenting swollen vestibule structure. As E. fasciatus eggs was pelagic, so chorionic surface was an uneven structures such as circular and fillar form nodules. Especially, various pores (0.15~0.55 μm, 230~270 pores) distributed at the only around micropyles, those pores radiately exhibited regular projection structures showing gill filament-shape. These ultrastructural characters of E. fasciatus eggs can be utilized in a taxonomical cue of grouper species.
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