대륙밭쥐(Clethrionomys rufocanus) 정자의 미세구조 관찰
Ultrastructural Observation on the Sperm of the Grey Red-blacked Vole, Clethrionomys rufocanus
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초록
Cricetinae 아과 Clethrionomys 아속에 속하는 한국산 대륙밭쥐 (Clethrionomys rufocanus)의 정자의 형태적 특징을 알아보기 위 하여 주사 및 투과형전자현미경으로 관찰하였다. C. rufocanus정 자머리의 모양은 날카롭게 굽은 하나의 dorsal hook를 가지며, 전체적인 머리모양은 마치 도끼모양의 형태를 취하고 있었다. C. rufocanus정자의 전체길이는 95.8 μm이었으며, 정자머리의 길이 는 7.8 μm, 꼬리는 88.0 μm이었다. 꼬리는 경부 (1.0 μm), 중편부 (22.0 μm), 주편부와 미부 (65.0 μm)로 각각 구성되어져 있었다. 분절주 (segmented columns)는 약 10~12개의 마디로 이루어져 있었으며, 미토콘드리아의 수는 약 170~178개 정도이었다. 첨체 후 덮개는 핵의 1/2을 점유하고 있었으며, 적도절은 핵 표면의 첨체 후 덮개(post-nuclear cap)와 첨체덮개(acrosomal cap) 사이 에 위치하고 있었다. 1, 5, 6번의 외측섬유들은 다른 것보다 더 크며, 섬유초 (fibrous sheath)와 종 지주(longitudinal column)은 주편부에서 관찰되어졌으며, 미부에서는 섬유초가 존재하지 않 았다. 이상의 결과로 미루어 보아, 정자두부와 꼬리의 형태적인 구조는 정자의 진화 패턴과 종을 분류하는데 유용한 정보를 제 공하리라 여겨진다.
Abstract
To investigate the morphological characteristics of spermatozoa of the grey red-blacked vole (Clethrionomys rufocanus) belongings to the subfamily Cricetinae, subgenus Clethrionomys were examined by scanning and transmission electron microscopes. The sperm head of C. rufocanus was an ax or hatchet in shape with a curved single dorsal hook. The total length of C. regulus sperm was 95.8 μm. The length of sperm head was 7.8 μm, and the tail (88.0 μm) consisted of four major segments: the neck (1.0 μm), middle piece (22.0 μm), and principal piece plus end piece (65.0 μm), respectively. The segmented columns were about 10~12 in number. The number of gyres of mitochondria ranged from about 170 to 178. The post-nuclear cap occupied about a half of nucleus. The equatorial segment is located between the post-nuclear cap segment and acrosomal cap on the nuclear surface. Nos. 1, 5 and 6 of the outer dense fibers were larger than the others. A fibrous sheath and longitudinal column of the principal piece were in evidence, but the fibrous sheath was not seen at the end piece. In conclusion, the morphological structures of sperm head and tail may be useful information to patterning of sperm evolution and classifying of species.
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