Expression Pattern of Early Growth Response Gene 1 during Olive Flounder (Paralichthys olivaceus) Embryonic Development
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The early growth response protein 1 (Egr-1) is a widely reported zinc finger protein and a well knowntranscription factor encoded by the Egr-1 gene, which plays key roles in many aspects of vertebrate embryogenesis and inadult vertebrates. The Egr-1 expression is important in the formation of the gill vascular system in flounders, which developsduring the post-hatching phase and is essential for survival during the juvenile period. However, the complete details of Egr-1expression during embryo development in olive flounder are not available. We assessed the expression patterns of Egr-1during the early development of olive flounders by using reverse transcription polymerase chain reaction (RT-PCR) analysis. Microscopic observations showed that gill filament formation corresponded with the Egr-1 expression. Thus, we showed thatEgr-1 plays a vital role in angiogenesis in the gill filaments during embryogenesis. Further, Egr-1 expression was found to bestrong at 5 days after hatching (DAH), in the development of the gill vascular system, and this strong expression level wasmaintained throughout all the development stages. Our findings have important implications with respect to the biological roleof Egr-1 and evolution of the first respiratory blood vessels in the gills of olive flounder. Further studies are required toelucidate the Egr-1-mediated stress response and to decipher the functional role of Egr-1 in developmental stages.
Abstract
The early growth response protein 1 (Egr-1) is a widely reported zinc finger protein and a well knowntranscription factor encoded by the Egr-1 gene, which plays key roles in many aspects of vertebrate embryogenesis and inadult vertebrates. The Egr-1 expression is important in the formation of the gill vascular system in flounders, which developsduring the post-hatching phase and is essential for survival during the juvenile period. However, the complete details of Egr-1expression during embryo development in olive flounder are not available. We assessed the expression patterns of Egr-1during the early development of olive flounders by using reverse transcription polymerase chain reaction (RT-PCR) analysis. Microscopic observations showed that gill filament formation corresponded with the Egr-1 expression. Thus, we showed thatEgr-1 plays a vital role in angiogenesis in the gill filaments during embryogenesis. Further, Egr-1 expression was found to bestrong at 5 days after hatching (DAH), in the development of the gill vascular system, and this strong expression level wasmaintained throughout all the development stages. Our findings have important implications with respect to the biological roleof Egr-1 and evolution of the first respiratory blood vessels in the gills of olive flounder. Further studies are required toelucidate the Egr-1-mediated stress response and to decipher the functional role of Egr-1 in developmental stages.
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