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Overview of Immunoelectron Microscopy

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초록

Immunoelectron microscopy using an antigen-antibody reaction in an electron microscope is a very useful tool to identify the components of a tissue in an electron microscope. Many researchers also use immunoelectron microscopy. Nonetheless, immunoelectron microscopy is rarely introduced systematically, and immunoelectron microscopy can be carried out without fully understanding the principles, and cases of poor understanding can often be seen in the vicinity. Therefore, in order to make it easier to understand, we will first introduce the principles of immunoelectron microscopy and describe practical methods.

Abstract

Immunoelectron microscopy using an antigen-antibody reaction in an electron microscope is a very useful tool to identify the components of a tissue in an electron microscope. Many researchers also use immunoelectron microscopy. Nonetheless, immunoelectron microscopy is rarely introduced systematically, and immunoelectron microscopy can be carried out without fully understanding the principles, and cases of poor understanding can often be seen in the vicinity. Therefore, in order to make it easier to understand, we will first introduce the principles of immunoelectron microscopy and describe practical methods.

참고문헌 (3)

  1. Chang B H, "A study on the localization of neurofascin in the myelinated rat sciatic nerve fibers", Korean J. Electron Microsc, 36, pp.131~140 (2006)
  2. Goldberg M W, "High-resolution scanning electron microscopy and immuno-gold labeling of the nuclear lamina and nuclear pore complex", Methods Mol. Biol, 1411, pp.441~459 (2016)
  3. Melo R C, "Pre-embedding immunogold labeling to optimize protein localization at subcellular compartiments and membrane microdomains of leukocytes", Nat. Protoc, 9, pp.2382~2394 (2014)

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