Current Status of Automatic Serial Sections for 3D Electron Microscopy
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
The automatic equipment for three-dimensional electron microscopy (3DEM) can acquire serial sections of a large sample in a relatively short time, and is especially suitable for the connectomics, which is a field related to understanding the brain structure as a whole. As many results obtained through 3DEM using automatic serial sections have been published in the field of brain research, many researchers continue to apply this technique to various samples. We reviewed the equipment for automatic serial sectioning, the block preparation method, the limitations of 3DEM, and future directions.
Abstract
The automatic equipment for three-dimensional electron microscopy (3DEM) can acquire serial sections of a large sample in a relatively short time, and is especially suitable for the connectomics, which is a field related to understanding the brain structure as a whole. As many results obtained through 3DEM using automatic serial sections have been published in the field of brain research, many researchers continue to apply this technique to various samples. We reviewed the equipment for automatic serial sectioning, the block preparation method, the limitations of 3DEM, and future directions.
참고문헌 (19)
- Bittermann A G, "Quantitative immuno-gold labelling and ultrastructural preservation after cryofixation (combined with different freeze-substitution and embedding protocols) and after chemical fixation and cryosectioning. Analysis of the secretory organelle matrix of Paramecium trichocysts", Histochemistry, 97, pp.421~429 (1992)
- Cabezon I, "Serial block-face scanning electron microscopy applied to study the trafficking of 8D3-coated gold nanoparticles at the bloodbrain barrier", Histochem. Cell Biol (2017)[DOI]
- Collman F, "Mapping synapses by conjugate light-electron array tomography", J. Neurosci, 35, pp.5792~5807 (2015)
- Denk W, "Serial block-face scanning electron microscopy to reconstruct three-dimensional tissue nanostructure", PLoS Biol, 2, pp.e329~ (2004)
- Horstmann H, "Photooxidation-guided ultrastructural identification and analysis of cells in neuronal tissue labeled with green fluorescent protein", PLoS One, 8, pp.e64764~ (2013)
- Hua Y, "Large-volume en-bloc staining for electron microscopy-based connectomics", Nat. Commun, 6, pp.7923~ (2015)
- Joesch M, "Reconstruction of genetically identified neurons imaged by serialsection electron microscopy", Elife, 5, pp.e15015~ (2016)
- Jung M K, "Techniques and applications of three-dimensional electron microscopy in entomological research", Entomol. Res, 46, pp.374~380 (2016)
- Kasthuri N, "Saturated reconstruction of a volume of neocortex", Cell, 162, pp.648~661 (2015)
- Gyu Hyun Kim, "Reconstruction of Neural Circuits Using Serial Block-Face Scanning Electron Microscopy", 한국현미경학회지, 46(2), pp.100~104 (2016), 한국현미경학회
- Lucas M S, "Bridging microscopes: 3D correlative light and scanning electron microscopy of complex biological structures", Methods Cell Biol, 111, pp.325~356 (2012)
- Martell J D, "Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy", Nat. Biotechnol, 30, pp.1143~1148 (2012)
- Miyazaki N, "Serial block-face scanning electron microscopy for three-dimensional analysis of morphological changes in mitochondria regulated by Cdc48p", p97ATPase, 2014, pp.187, 187~193
- Mun J Y, "A low fluence Q-switched Nd:YAG laser modifies the 3D structure of melanocyte and ultrastructure of melanosome by subcellular-selective photothermolysis", J. Electron. Microsc. (Tokyo), 60, pp.11~18 (2011)
- Mun J Y, "Three-dimensional image analysis of melanocyte in human skin", Biophys. J, 112, pp.578a~ (2017)
- 문지영, "생물학분야에서 Cryo-electron Tomography 활용기법", 한국현미경학회지, 38(2), pp.73~79 (2008), 한국현미경학회
- Nguyen H B, "Conductive resins improve charging and resolution of acquired images in electron microscopic volume imaging", Sci. Rep, 6, pp.23721~ (2016)
- Shami G J, "3-D EM exploration of the hepatic microarchitecture-lessons learned from large-volume in situ serial sectioning", Sci. Rep, 6, pp.36744~ (2016)
- Tapia J C, "High-contrast en bloc staining of neuronal tissue for field emission scanning electron microscopy", Nat. Protoc, 7, pp.193~206 (2012)