Immunohistochemical Localization of Anoctamin 1 in the Mouse Cerebellum
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Since a transmembrane protein, TMEM16A, also called anoctamin 1 (ANO1), was identified as a bona fide calcium (Ca2+)-activated chloride (Cl–) channel (CaCC), there have been many reports on its expression and function. However, limited information on ANO1 expression and function in the brain is still available. In this study, we tried to re-examine expression patterns of ANO1 in the mouse cerebellum and further characterize ANO1-expressing components by immunohistochemical analyses. Strong ANO1 immunoreactivity was observed as large puncta in the granule cell layer and weak to moderate immunoreactivities were observed as small puncta in the molecular and Purkinje cell layers. Double-label experiments revealed that ANO1 did not colocalize with cerebellar neuronal population markers, such as anti-calbindin and anti-NeuN, while it colocalized or intermingled with a presynaptic marker, anti-synaptophysin. These results demonstrate that ANO1 is mainly localized at presynaptic terminals in the cerebellum and involved in synaptic transmission and modulation in cerebellar information processing.
Abstract
Since a transmembrane protein, TMEM16A, also called anoctamin 1 (ANO1), was identified as a bona fide calcium (Ca2+)-activated chloride (Cl–) channel (CaCC), there have been many reports on its expression and function. However, limited information on ANO1 expression and function in the brain is still available. In this study, we tried to re-examine expression patterns of ANO1 in the mouse cerebellum and further characterize ANO1-expressing components by immunohistochemical analyses. Strong ANO1 immunoreactivity was observed as large puncta in the granule cell layer and weak to moderate immunoreactivities were observed as small puncta in the molecular and Purkinje cell layers. Double-label experiments revealed that ANO1 did not colocalize with cerebellar neuronal population markers, such as anti-calbindin and anti-NeuN, while it colocalized or intermingled with a presynaptic marker, anti-synaptophysin. These results demonstrate that ANO1 is mainly localized at presynaptic terminals in the cerebellum and involved in synaptic transmission and modulation in cerebellar information processing.
참고문헌 (47)
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