17Beta-Estradiol Regulates NUCB2/ Nesfatin-1 Expression in Mouse Oviduct
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NUCB2/nesfatin-1 known to regulate appetite and energy homeostasis is expressed not only in the hypothalamus, but also in various organs and tissues. Our previous reports also demonstrated that NUCB2/nesfatin-1 was expressed in the reproductive organs, including the ovaries, uterus, and testes of mice. However, it is yet known whether NUCB2/nesfatin-1 is expressed in the oviduct and how its expression is regulated. Therefore, we investigated the expression of NUCB2/nesfatin-1 in the oviduct and its expression is regulated by gonadotropin. Immunohistochemical staining results showed that nesfatin-1 protein was localized in epithelial cells of the oviduct. As a result of quantitative real-time PCR (qRT-PCR) and Western blot, NUCB2/nesfatin-1 was detected strongly in the oviducts. During the estrus cycle, NUCB2/nesfatin-1 expression in the oviducts was markedly higher in the proestrus stage than in other estrus stages. In order to elucidate whether the expression of NUCB2 mRNA is controlled by the gonadotropins, we injected PMSG and hCG and measured NUCB2 mRNA level in the oviduct after injection. Its level was increased in the oviduct after PMSG injection, but no significant change after hCG injection. In addition, NUCB2 mRNA levels were markedly reduced after ovariectomy, while recovered after 17β-estradiol (E2) injection, but not by progesterone (P4). This study demonstrated that NUCB2/nesfatin-1 is highly expressed in the oviduct of mouse and its expression is regulated by E2 secreted by the ovaries. These results suggest that NUCB2/nesfatin-1 expressed by the oviduct may affect the function of the oviduct regulated by the ovaries.
Abstract
NUCB2/nesfatin-1 known to regulate appetite and energy homeostasis is expressed not only in the hypothalamus, but also in various organs and tissues. Our previous reports also demonstrated that NUCB2/nesfatin-1 was expressed in the reproductive organs, including the ovaries, uterus, and testes of mice. However, it is yet known whether NUCB2/nesfatin-1 is expressed in the oviduct and how its expression is regulated. Therefore, we investigated the expression of NUCB2/nesfatin-1 in the oviduct and its expression is regulated by gonadotropin. Immunohistochemical staining results showed that nesfatin-1 protein was localized in epithelial cells of the oviduct. As a result of quantitative real-time PCR (qRT-PCR) and Western blot, NUCB2/nesfatin-1 was detected strongly in the oviducts. During the estrus cycle, NUCB2/nesfatin-1 expression in the oviducts was markedly higher in the proestrus stage than in other estrus stages. In order to elucidate whether the expression of NUCB2 mRNA is controlled by the gonadotropins, we injected PMSG and hCG and measured NUCB2 mRNA level in the oviduct after injection. Its level was increased in the oviduct after PMSG injection, but no significant change after hCG injection. In addition, NUCB2 mRNA levels were markedly reduced after ovariectomy, while recovered after 17β-estradiol (E2) injection, but not by progesterone (P4). This study demonstrated that NUCB2/nesfatin-1 is highly expressed in the oviduct of mouse and its expression is regulated by E2 secreted by the ovaries. These results suggest that NUCB2/nesfatin-1 expressed by the oviduct may affect the function of the oviduct regulated by the ovaries.
참고문헌 (32)
- Archanco M, "Expression of leptin and adiponectin in the rat oviduct", J Histochem Cytochem, 55, pp.1027~1037 (2007)
- Barnikol-Watanabe S, "Human protein NEFA, a novel DNA binding", EF-hand, Biol Chem Hoppe Seyler, pp.1994, 375[DOI]
- Boatman DE, "Responses of gametes to the oviductal environment", Hum Reprod, 12, pp.133~149 (1997)
- Catak Z, "NUCB2", nesfatin-1 in the blood and follicular fluid in patients with polycystic ovary syndrome and poor ovarian response, 2019, pp.20, 225~230
- Chiu PC, "Cumulus oophorus-associated glycodelin-C displaces sperm-bound glycodelin-A and-F and stimulates spermatozoa-zona pellucida binding", J Biol Chem, 282, pp.5378~5388 (2007)
- Choudhary S, "Structural and functional characterization of buffalo oviduct-specific glycoprotein (OVGP1) expressed during estrous cycle", Biosci Rep, 39 (2019)
- Chung Y, "Progesterone and 17β-estradiol regulate expression of nesfatin-1", NUCB2 in mouse pituitary gland, 2015, pp.63, 4~9
- Craig JA, "Leptin enhances porcine preimplantation embryo development in vitro", Mol Cell Endocrinol, 229, pp.141~147 (2005)
- Deaver SE, "Localization of ghrelin and its receptor in the reproductive tract of holstein heifers", J Dairy Sci, 96, pp.150~157 (2013)
- García-Galiano D, "Expanding roles of NUCB2", nesfatin-1 in neuroendocrine regulation, 2010, pp.45, 281~290
- Gonzalez R, "Molecular, cellular and physiological evidences for the anorexigenic actions of nesfatin-1 in goldfish", PLoS One, 5, pp.e15201~ (2010)
- Gonzalez R, "Pancreatic beta cells colocalize insulin and pronesfatin immunoreactivity in rodents", Biochem Biophys Res Commun, 381, pp.643~648 (2009)
- Jiang S, "Developmental expression and distribution of nesfatin-1", NUCB2 in the canine digestive system, 2016, pp.118, 90~96
- Kawamura K, "The role of leptin during the development of mouse preimplantation embryos", Mol Cell Endocrinol, 202, pp.185~189 (2003)
- Kawamura K, "Leptin promotes the development of mouse preimplantation embryos in vitro", Endocrinology, 143, pp.1922~1931 (2002)
- 김진희, "The Tissue Distribution of Nesfatin-1", NUCB2 in Mouse, 2014, pp.18(4), 301~309[DOI]
- Kim J, "Gonadotropin regulates NUCB2", nesfatin-1expression in the mouse ovary and uterus, 2019, pp.513, 602~607
- Lamy J, "Steroid hormones in bovine oviductal fluid during the estrous cycle", Theriogenology, 86, pp.1409~1420 (2016)
- Li S, "Oviduct : roles in fertilization and early embryo development", J Endocrinol, 232, pp.R1~R26 (2017)
- Liu Q, "Osteopontin is expressed in the oviduct and promotes fertilization and preimplantation embryo development of mouse", Zygote, 23, pp.622~630 (2015)
- Luque EM, "Role of ghrelin in fertilization, early embryo development, and implantation periods", Reproduction, 148, pp.159~167 (2014)
- Miura K, "Molecular cloning of nucleobindin, a novel DNAbinding protein that contains both a signal peptide and a leucine zipper structure", Biochem Biophys Res Commun, 187, pp.375~380 (1992)
- Nishie T, "Acute stimulation of a smooth muscle constrictor by oestradiol-17β via GPER1 in bovine oviducts", Reprod Domest Anim, 53, pp.326~332 (2018)
- O’Day-Bowman MB, "Association of oviduct-specific glycoproteins with human and baboon(Papio anubis)ovarian oocytes and enhancement of human sperm binding to human hemizonae following in vitro incubation", Biol Reprod, 54, pp.60~69 (1996)
- Oh-I S, "Identification of nesfatin-1 as a satiety molecule in the hypothalamus", Nature, 443, pp.709~712 (2006)
- Prinz P, "Peripheral and central localization of the nesfatin-1 receptor using autoradiography in rats", Biochem Biophys Res Commun, 470, pp.521~527 (2016)
- Rapisarda JJ, "Immunological characterization and immunocytochemical localization of an oviduct-specific glycoprotein in the human", J Clin Endocrinol Metab, 76, pp.1483~1488 (1993)
- Shimizu H, "Transgenic mice overexpressing nesfatin", nucleobindin-2are susceptible to high-fat diet-induced obesity, 2016, pp.6, e201~
- Stengel A, "Nesfatin-130-59 but not the N-and C terminal fragments, nesfatin-11-29 and nesfatin-160-82 injected intracerebroventricularly decreases dark phase food intake by increasing inter-meal intervals in mice", Peptides, 35, pp.143~148 (2012)
- Xi L, "Expression of leptin receptor in the oviduct of Chinese brown frog(Rana dybowskii)", Am J Physiol Regul Integr Comp Physiol, 312, pp.R912~R918 (2017)
- Yeung WS, "Effects of glycodelins on functional competence of spermatozoa", J Reprod Immunol, 83, pp.26~30 (2009)
- Zhang AQ, "Expression of nesfatin-1", NUCB2 in rodent digestive system, 2010, pp.16, 1735~1741