The Tissue Distribution of Nesfatin-1/NUCB2 in Mouse
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Nesfatin-1, an anorexic nucleobindin-2 (NUCB2)-derived hypothalamic peptide, controls appetite and energymetabolism. Recent studies show that nesfatin-1/NUCB2 is expressed not only in the brain but also in gastric and adiposetissues. Thus, we investigated the distributions of nesfatin-1/NUCB2 in various tissues of male and female mice by real-timePCR, western blotting, and immunohistochemical staining. Real-time PCR analyses showed that NUCB2 mRNA waspredominantly expressed in the pituitary and at lower levels in the hypothalamus, spleen, thymus, heart, liver, and muscle ofboth male and female mice. Expression was much higher in reproductive organs, such as the testis, epididymis, ovary, anduterus, than in the hypothalamus. Western blot analysis of the nesfatin-1 protein level showed similar results to the real-timePCR analyses in both male and female mice. These results suggest that nesfatin-1/NUCB2 have widespread physiologicaleffects in endocrine and non-endocrine organs. In addition, immunohistochemical staining revealed that nesfatin-1 waslocalized in interstitial cells, including Leydig cells and in the columnar epithelium of the epididymis. Nesfatin-1 was alsoexpressed in theca cells and interstitial cells in the ovary and in epithelial cells of the endometrium and uterine glands in theuterus. These results suggest that nesfatin-1 is a novel potent regulator of steroidogenesis and gonadal function in male andfemale reproductive organs. Further studies are required to elucidate the functions of nesfatin-1 in various organs of male andfemale mice.
Abstract
Nesfatin-1, an anorexic nucleobindin-2 (NUCB2)-derived hypothalamic peptide, controls appetite and energymetabolism. Recent studies show that nesfatin-1/NUCB2 is expressed not only in the brain but also in gastric and adiposetissues. Thus, we investigated the distributions of nesfatin-1/NUCB2 in various tissues of male and female mice by real-timePCR, western blotting, and immunohistochemical staining. Real-time PCR analyses showed that NUCB2 mRNA waspredominantly expressed in the pituitary and at lower levels in the hypothalamus, spleen, thymus, heart, liver, and muscle ofboth male and female mice. Expression was much higher in reproductive organs, such as the testis, epididymis, ovary, anduterus, than in the hypothalamus. Western blot analysis of the nesfatin-1 protein level showed similar results to the real-timePCR analyses in both male and female mice. These results suggest that nesfatin-1/NUCB2 have widespread physiologicaleffects in endocrine and non-endocrine organs. In addition, immunohistochemical staining revealed that nesfatin-1 waslocalized in interstitial cells, including Leydig cells and in the columnar epithelium of the epididymis. Nesfatin-1 was alsoexpressed in theca cells and interstitial cells in the ovary and in epithelial cells of the endometrium and uterine glands in theuterus. These results suggest that nesfatin-1 is a novel potent regulator of steroidogenesis and gonadal function in male andfemale reproductive organs. Further studies are required to elucidate the functions of nesfatin-1 in various organs of male andfemale mice.
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