분자표지 이용 Polyphenol Oxidase 저활성 국산밀 유전자원 선발
Selection of Korean Wheat Germplasms with Low Polyphenol Oxidase using Polyphenol Oxidase-Specific DNA Markers
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초록
The color of wheat flour and dough affect significantly on the quality of wheat. The major factor causing darkening of dough color is activity of polyphenol oxidase (PPO). Therefore, we examined the relationship between the PPO activities and the genotypes of the PPO-specific molecular markers in Korean wheat cultivars, and tested the usefulness of the selected markers for the selection of Korean wheat germplasm lines low in PPO activity. Five sequence-tagged site (STS) markers specific to wheat PPO genes, PPO-05, PPO-16, PPO-18, PPO-30 and PPO-43, were examined for their levels of polymorphism in Korean wheat cultivars. The genotypes of the marker PPO-18, which is linked to Ppo-A1 locus, were correlated significantly highly with the levels of grain PPO activity. Furthermore, Korean wheat cultivars could be classified into the high or low PPO activity group by the allele type of Ppo-A1 which was differentiated by the PPO-18. Grain PPO activity was lower about twice in the cultivars with Ppo-A1b allele than in those with Ppo-A1a allele. The cultivars with Ppo-A1b allele had lower grain weight and wide grain width, also they had less ash, protein, Fe and total polyphenol than those with Ppo-A1a allele. Thus, the lightness of flour and dough sheet was brighter in the cultivars with Ppo-A1b allele. The four wheat cultivars including Suwon252, which had lowest PPO activity and Ppo-A1b allele, were selected from 200 genetic lines as valuable genetic resources for the breeding of wheat variety with bright white-colored flour.
Abstract
The color of wheat flour and dough affect significantly on the quality of wheat. The major factor causing darkening of dough color is activity of polyphenol oxidase (PPO). Therefore, we examined the relationship between the PPO activities and the genotypes of the PPO-specific molecular markers in Korean wheat cultivars, and tested the usefulness of the selected markers for the selection of Korean wheat germplasm lines low in PPO activity. Five sequence-tagged site (STS) markers specific to wheat PPO genes, PPO-05, PPO-16, PPO-18, PPO-30 and PPO-43, were examined for their levels of polymorphism in Korean wheat cultivars. The genotypes of the marker PPO-18, which is linked to Ppo-A1 locus, were correlated significantly highly with the levels of grain PPO activity. Furthermore, Korean wheat cultivars could be classified into the high or low PPO activity group by the allele type of Ppo-A1 which was differentiated by the PPO-18. Grain PPO activity was lower about twice in the cultivars with Ppo-A1b allele than in those with Ppo-A1a allele. The cultivars with Ppo-A1b allele had lower grain weight and wide grain width, also they had less ash, protein, Fe and total polyphenol than those with Ppo-A1a allele. Thus, the lightness of flour and dough sheet was brighter in the cultivars with Ppo-A1b allele. The four wheat cultivars including Suwon252, which had lowest PPO activity and Ppo-A1b allele, were selected from 200 genetic lines as valuable genetic resources for the breeding of wheat variety with bright white-colored flour.
참고문헌 (21)
- AACC, "Approved methods of the American Association of Cereal Chemists, 9th edn" (2000), AACC
- Anderson JV, "An improved whole-seed assay for screening wheat germplasm for polyphenol oxidase activity", Crop Sci, 41, pp.1697~1705 (2001)
- Baik BK, "Role and contribution of starch and protein contents and quality to texture profile analysis of oriental noodles", Cereal Chem, 71, pp.315~320 (1994)
- Baik BK, "Discoloration of dough for oriental noodles", Cereal Chem, 72, pp.198~205 (1995)
- Cary JW, "Cloning and characterization of cDNAs coding for Vicia faba polyphenol oxidase", Plant Mol. Biol, 20, pp.245~253 (1992)
- Demeke T, "Wheat polyphenol oxidase: distribution and genetic mapping in three inbred line populations", Crop Sci, 41, pp.1750~1757 (2001)
- Demeke T, "Molecular characterization of wheat polyphenol oxidase (PPO)", Theor. Appl. Genet, 104, pp.813~818 (2002)
- Feillet P, "Pasta brownness: an assessment", J. Cereal Sci, 32, pp.215~233 (2000)
- He XY, "Cloning and phylogenetic analysis of polyphenol oxidase genes in common wheat and related species", Genet. Resour. Crop Evol, 56, pp.311~321 (2009)
- He XY, "Allelic variation of polyphenol oxidase (PPO) genes located on chromosomes 2A and 2D and development of functional markers for the PPO genes in common wheat", Theor. Appl. Genet, 115, pp.47~58 (2007)
- Chon-Sik Kang, "Effect of Polyphenol Oxidase Activity on Discoloration of Noodle Dough Sheet Prepared from Korean Wheats", 한국작물학회지, 53(2), pp.187~195 (2008), 한국작물학회
- Ministry of Food, "Agriculture, Forestry and Fisheries", ~ (2009)
- Park CS, "Influence of puroindoline genotypes on grain characteristics, hysic-chemical properties of flour and end-use quality of Korean wheats", Breed. Sci, 60, pp.233~242 (2010)
- Raman R, "Functional gene markers for polyphenol oxidase locus in bread wheat (Triticum aestivum L.)", Mol. Breeding, 19, pp.315~328 (2007)
- SAS, "SAS User’s Guide" (2000), The Institute
- Sun DJ, "A novel STS marker for polyphenol oxidase activity in bread wheat", Mol. Breeding, 16, pp.209~218 (2005)
- van Gelder CW, "Sequence and structural features of plant and fungal tyrosinases", Phytochemistry, 45, pp.1309~1323 (1997)
- Wang XB, "Gene markers for grain polyphenol oxidase activity in common wheat", Mol. Breeding, 23, pp.163~170 (2009)
- Watanabe N, "Genetic mapping of the gene affecting polyphenol oxidase activity in tetraploid durum wheat", J. Appl. Genet, 47, pp.201~205 (2006)
- Watanabe N, "Inheritance and chromosomal location of the homoeologous genes affecting phenol colour reaction of kernels in durum wheat", Euphytica, 139, pp.87~93 (2004)
- Wrigley CW, "Genetic control of factors regulating the phenol reaction of wheat and rye grain", Wheat Inf. Serv, 40, pp.6~10 (1975)