밀 고분자 글루테닌 조성 분석을 위한 유전자 특이 분자표지 선발
Validation and Selection of Functional Allele-specific Molecular Markers to Analyze High-Molecular-Weight Glutenin Subunit Composition in Wheat
1
조회
0
다운로드
4
인용
0
참고문헌
키워드
초록
The high-molecular-weight glutenin subunit (HMW-GS) composition of wheat is the main factor controlling gluten strength related to bread baking quality. Reported molecular markers for HMW-GS were validated and selected for improved breeding efficiency in South Korean wheat breeding programs. Sodium dodecyl sulfate polyacrylamide gel electrophoresis, lab-on-a-chip electrophoresis, sequence-tagged site (STS) markers, and Kompetitive Allele-Specific PCR were performed to re-evaluate the known HMW-GS of 14 wheat cultivars. Glu-A1b and Glu-A1c alleles were separated by the STS marker, UMN19, and KASP marker, namely Glu-Ax1/2*_SNP, at Glu-1 loci. At the Glu-B1 locus, Glu1-By8 and Glu1-By9 could be distinguished from Glu-B1b and Glu-B1c alleles by two STS markers, namely ZSBy8 and ZSBy9a, respectively. Glu1-Bx17 and Glu1-7OE could respectively be separated from non-Glu-B1i and non-Glu-B1al alleles by cauBx642 and BX7OE_866_SNP. The Glu-D1d allele, used to determine bread baking quality, could easily be distinguished from other alleles by Glu-D1d_SNP at Glu-D1 loci. Validated molecular markers in this study could therefore be used to select wheat lines for good bread baking quality in South Korean wheat breeding programs.
Abstract
The high-molecular-weight glutenin subunit (HMW-GS) composition of wheat is the main factor controlling gluten strength related to bread baking quality. Reported molecular markers for HMW-GS were validated and selected for improved breeding efficiency in South Korean wheat breeding programs. Sodium dodecyl sulfate polyacrylamide gel electrophoresis, lab-on-a-chip electrophoresis, sequence-tagged site (STS) markers, and Kompetitive Allele-Specific PCR were performed to re-evaluate the known HMW-GS of 14 wheat cultivars. Glu-A1b and Glu-A1c alleles were separated by the STS marker, UMN19, and KASP marker, namely Glu-Ax1/2*_SNP, at Glu-1 loci. At the Glu-B1 locus, Glu1-By8 and Glu1-By9 could be distinguished from Glu-B1b and Glu-B1c alleles by two STS markers, namely ZSBy8 and ZSBy9a, respectively. Glu1-Bx17 and Glu1-7OE could respectively be separated from non-Glu-B1i and non-Glu-B1al alleles by cauBx642 and BX7OE_866_SNP. The Glu-D1d allele, used to determine bread baking quality, could easily be distinguished from other alleles by Glu-D1d_SNP at Glu-D1 loci. Validated molecular markers in this study could therefore be used to select wheat lines for good bread baking quality in South Korean wheat breeding programs.
참고문헌 (32)
- Ahmad M, "Molecular marker-assisted selection of HMW glutenin alleles related to wheat bread quality by PCR-generated DNA markers", Theor Appl Genet, 101, pp.892~896 (2002)
- Allen AM, "Discovery and development of exome-based, co-dominant single nucleotide polymorphism markers in hexaploid wheat(Triticum aestivum L. )", Plant Biotechnol J, 11, pp.279~295 (2013)
- Anderson O, "The characterization and comparative analysis of high-molecular-weight glutenin genes from genomes A and B of a hexaploid bread wheat", Theor Appl Genet, 77, pp.689~700 (1989)
- Anjum FM, "Wheat gluten: high molecular weight glutenin subunits--structure, genetics, and relation to dough elasticity", J Food Sci, 72, pp.R56~R63 (2007)
- Ayalew H, "Comparison of TaqMan, KASP and rhAmp SNP genotyping platforms in hexaploid wheat", PloS one, 14, pp.e0217222~ (2019)
- 최창현, "이형접합 Pinb-D1 유전자형이 밀가루 이화학적 특성 및 가공 적성에 미치는 영향", 한국육종학회지, 52(2), pp.131~139 (2020), 한국육종학회[DOI]
- De Bustos A, "Molecular characterisation of the inactive allele of the gene Glu-A1and the development of a set of AS-PCR markers for HMW glutenins of wheat", Theor Appl Genet, 100, pp.1085~1094 (2000)
- Gao S, "High-molecular-weight glutenin subunit compositions in current Chinese commercial wheat cultivars and the implication on Chinese wheat breeding for quality", Cereal Chem, 97, pp.762~771 (2020)
- Geisslitz S, "Mass spectrometry of in-gel digests reveals differences in amino acid sequences of high-molecular-weight glutenin subunits in spelt and emmer compared to common wheat", Anal Bioanal Chem, 412, pp.1277~1289 (2020)
- Gilbert SM, "Expression and characterisation of a highly repetitive peptide derived from a wheat seed storage protein", Biochim Biophys Acta, 1479, pp.135~146 (2000)
- Jang YR, "Improved method for reliable HMW-GS identification by RP-HPLC and SDS-PAGE in common wheat cultivars", Molecules, 22, pp.1055~ (2017)
- 강주원, "Development and Validation of KASP Markers for Stv-bi, a Rice Stripe Virus Resistance Gene in Rice (Oryza sativa L.)", Plant Breeding and Biotechnology, 8(2), pp.196~201 (2020), 한국육종학회[DOI]
- Lei Z, "Y-type gene specific markers for enhanced discrimination of high-molecular weight glutenin alleles at the Glu-B1 locus in hexaploid wheat", J Cereal Sci, 43, pp.94~101 (2006)
- LiuS, "New DNA markers for high molecular weight glutenin subunits in wheat", Theor Appl Genet, 118, pp.177~183 (2008)
- Ma W, "Multiplex-PCR typing of high molecular weight glutenin alleles in wheat", Euphytica, 134, pp.51~60 (2003)
- Mondal S, "Use of near-isogenic wheat lines to determine the glutenin composition and functionality requirements for flour tortillas", J Agr Food Chem, 56, pp.179~184 (2008)
- Murray M, "Rapid isolation of high molecular weight plant DNA", Nucleic Acids Res, 8, pp.4321~4326 (1980)
- Ortolan F, "Protein characteristics that affect the quality of vital wheat gluten to be used in baking : A review", Compr Rev Food Sci F, 16, pp.369~381 (2017)
- Payne PI, "Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1, and Glu-D1 which code for high-molecular-weight subunits of glutenin in hexaploid wheat", Cereal Res Commun, 11, pp.29~35 (1983)
- Peng Y, "Two novel y-type high molecular weight glutenin genes in Chinese wheat landraces of the yangtze-river region", PloS One, 10, pp.e0142348~ (2015)
- Ragupathy R, "Evolutionary origin of the segmental duplication encompassing the wheat GLU-B1 locus encoding the overexpressed Bx7(Bx7OE)high molecular weight glutenin subunit", Theor Appl Genet, 116, pp.283~296 (2008)
- Rai R, "Application of allele-specific(AS-PCR)marker for identification of high-molecular-weight glutenin subunits(HMW-GS)at the GluB-1 locus in bread wheat(Triticum aestivum L. )", Adv Crop Sci Tech, 6, pp.387~ (2018)
- Rasheed A, "Development and validation of KASP assays for genes underpinning key economic traits in bread wheat", Theor Appl Genet, 129, pp.1843~1860 (2016)
- Ravel C, "SNP markers for early identification of high molecular weight glutenin subunits(HMW-GSs)in bread wheat", Theor Appl Genet, 133, pp.751~770 (2020)
- Roy N, "Expressed Ay HMW glutenin subunit in Australian wheat cultivars indicates a positive effect on wheat quality", J Cereal Sci, 79, pp.494~500 (2018)
- Shewry PR, "The contribution of wheat to human diet and health", Food Energy Secur, 4, pp.178~202 (2015)
- Singh NK, "A simplified SDS-PAGE procedure for separating LMW subunits of glutenin", J Cereal Sci, 14, pp.203~208 (1991)
- Tan CT, "Development and validation of KASP markers for the greenbug resistance gene Gb7 and the Hessian fly resistance gene H32 in wheat", Theor Appl Genet, 130, pp.1867~1884 (2017)
- XuQ, "PCR-based markers for identification of HMW-GS at Glu-B1x loci in common wheat", J Cereal Sci, 47, pp.394~398 (2008)
- Yasmeen F, "Genetic divergence for high-molecular weight glutenin subunits(HMW-GS)in indigenous landraces and commercial cultivars of bread wheat of Pakistan", Genet Mol Res, 14, pp.4829~4839 (2015)
- Yuan Z, "The detection of a de novo allele of the Glu-1Dx gene in wheat-rye hybrid offspring", Theor Appl Genet, 127, pp.2173~2182 (2014)
- Zhang P, "Effect of high molecular weight glutenin subunit composition in common wheat on dough properties and steamed bread quality", J Sci Food Agr, 94, pp.2801~2806 (2014)