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Multiplex STS-SSR 마커를 활용한 국산밀 품종 판별

Identification of Korean Wheat Cultivars Using Multiplex STS-SSR Markers

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참고문헌

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주제분류농학

초록

This study aimed to develop an agarose gel-based multiplex PCR assay using sequence-tagged site (STS) and simple sequencerepeat (SSR) markers that can differentiate Korean wheat cultivars. Forty-nine Korean wheat cultivars were primarily classified based on seedcoat color into red (36) and white (13) groups. Red wheat cultivars were further differentiated by three multiplex PCRs using molecular markersfor Ppo-A1/Vrn-D1a/Rht-B1b, Glu-A3ac/TaCwi-A1b/Lr34, and Glu-A1ac/Glu-B1b/KWSM003/TaSE96. Similarly, white wheat cultivars werefurther differentiated using two multiplex PCRs using the molecular markers for Ppo-A1/Vrn-D1a/Pina-D1a and Ppo-B1/Glu-B3h. A multiplexPCR assay using molecular markers for Glu-A1b/Glu-D1d/Wx-B1 was developed to differentiate four Korean wheat cultivars used as governmentcertified seeds: Baekkang, Hwanggeumal, Keumkang, and Saekeumkang. A multiplex PCR assay using molecular markers for Glu-B3h andPin-D1a was used for colored wheat cultivars, Arijinheuk, Ariheuk, and Chinese colored wheat. The multiplex PCR assays developed in thisstudy can provide useful molecular tools for differentiating Korean wheat cultivars, developing wheat seed management systems, and guaranteeingwheat seeds in Korea.

Abstract

This study aimed to develop an agarose gel-based multiplex PCR assay using sequence-tagged site (STS) and simple sequencerepeat (SSR) markers that can differentiate Korean wheat cultivars. Forty-nine Korean wheat cultivars were primarily classified based on seedcoat color into red (36) and white (13) groups. Red wheat cultivars were further differentiated by three multiplex PCRs using molecular markersfor Ppo-A1/Vrn-D1a/Rht-B1b, Glu-A3ac/TaCwi-A1b/Lr34, and Glu-A1ac/Glu-B1b/KWSM003/TaSE96. Similarly, white wheat cultivars werefurther differentiated using two multiplex PCRs using the molecular markers for Ppo-A1/Vrn-D1a/Pina-D1a and Ppo-B1/Glu-B3h. A multiplexPCR assay using molecular markers for Glu-A1b/Glu-D1d/Wx-B1 was developed to differentiate four Korean wheat cultivars used as governmentcertified seeds: Baekkang, Hwanggeumal, Keumkang, and Saekeumkang. A multiplex PCR assay using molecular markers for Glu-B3h andPin-D1a was used for colored wheat cultivars, Arijinheuk, Ariheuk, and Chinese colored wheat. The multiplex PCR assays developed in thisstudy can provide useful molecular tools for differentiating Korean wheat cultivars, developing wheat seed management systems, and guaranteeingwheat seeds in Korea.

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