S-allele Specific PCR 분석에 의한 사과와 꽃사과 품종의 자가불화합성 유전자형 동정
Identification of Self-incompatibility Genotypes in Apple and Crabapple Cultivars by S-allele Specific PCR Analysis
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참고문헌
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초록
Apple (Malus ☓ domestica Borkh.) has gametophytic self-incompatibility (S) controlled by the multi-allelic S-locus. In the present study, S-genotypes of 24 apple cultivars including newly released Korean cultivars and seven crabapple cultivarswere identified using S-allele specific polymerase chain reaction analysis. Twelve different S-alleles (S1, S2, S3, S5, S7, S9, S10,S16, S21, S23, S26, and S29) from 31 apple and crabapple cultivars were identified using 23 S-allele specific primers. Among them,S1 (41.7%), S3 (58.3%), S7 (29.2%), and S9 (54.2%) S-alleles were found to be common in 24 apple cultivars. The newly releasedKorean cultivars ‘Arisoo’ and ‘Hwangok’ were genetyped as S3S7 and S3S9, respectively. S-genotypes information obtained fromthe present study will be useful to select proper pollinzers for stable production of apple fruit and to design cross of breedingprograms.
Abstract
Apple (Malus ☓ domestica Borkh.) has gametophytic self-incompatibility (S) controlled by the multi-allelic S-locus. In the present study, S-genotypes of 24 apple cultivars including newly released Korean cultivars and seven crabapple cultivarswere identified using S-allele specific polymerase chain reaction analysis. Twelve different S-alleles (S1, S2, S3, S5, S7, S9, S10,S16, S21, S23, S26, and S29) from 31 apple and crabapple cultivars were identified using 23 S-allele specific primers. Among them,S1 (41.7%), S3 (58.3%), S7 (29.2%), and S9 (54.2%) S-alleles were found to be common in 24 apple cultivars. The newly releasedKorean cultivars ‘Arisoo’ and ‘Hwangok’ were genetyped as S3S7 and S3S9, respectively. S-genotypes information obtained fromthe present study will be useful to select proper pollinzers for stable production of apple fruit and to design cross of breedingprograms.
참고문헌 (17)
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