비타민A 강화벼의 환경위해성 평가 : 4세대의 후대안정성 연구
Evaluation and Assessment of Biosafety Carotenoid-biofortified Rice (PAC) : Progeny Stability Study for Four Generations
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The carotenoid-biofortified (PAC) rice was developed and confirmed to synthesis carotenoids (zeaxanthin, α-carotene and β-carotene) in rice endosperm. For biosafety assessment of PAC rice, the stability of the insert genes in carotenoidbiofortified rice at genomic and protein expression level through different generations (T3~T6) was studied. Southern blot analysis confirmed that the multi-generation stability of the genes : PAC gene (CrtI and Psy), promoters (35S and Glb), terminators (NOS and PinII), selectable marker (bar) and MAR gene. It was also confirmed that the backbone DNA containing antibacterial gene (aadA) was not present in PAC rice. Immuno-strip assay (PAT) showed that the bar gene was stably expressed through generations. HPLC analysis confirmed that carotenoids were consistently detected through T3~T6 generations. These results will be filed to biosafety assessment document of PAC rice.
Abstract
The carotenoid-biofortified (PAC) rice was developed and confirmed to synthesis carotenoids (zeaxanthin, α-carotene and β-carotene) in rice endosperm. For biosafety assessment of PAC rice, the stability of the insert genes in carotenoidbiofortified rice at genomic and protein expression level through different generations (T3~T6) was studied. Southern blot analysis confirmed that the multi-generation stability of the genes : PAC gene (CrtI and Psy), promoters (35S and Glb), terminators (NOS and PinII), selectable marker (bar) and MAR gene. It was also confirmed that the backbone DNA containing antibacterial gene (aadA) was not present in PAC rice. Immuno-strip assay (PAT) showed that the bar gene was stably expressed through generations. HPLC analysis confirmed that carotenoids were consistently detected through T3~T6 generations. These results will be filed to biosafety assessment document of PAC rice.
참고문헌 (11)
- Datta K, "Bioengineered“golden” indica rice cultivars with β-carotene metabolism in the endosperm with hygromycin and mannose selection systems", Plant Biotech J, 1, pp.81~90 (2003)
- De Vries J, "Microbial horizontal gene transfer and the DNA release from transgenic crop plants", Plant and Soil, 266, pp.91~104 (2004)
- Ha SH, "Application of two bicistronic systems involving 2A and IRES sequences to the biosynthesis of carotenoids in rice endosperm", PLANT BIOTECHNOLOGY JOURNAL, 8(8), pp.928~938, WILEY-BLACKWELL PUBLISHING
- Hoa T, "Golden Indica and Japonica rice lines amenable to deregulation", Plant Physiol, 133, pp.161~169 (2003)
- The global status of commercialized biotech, "GM crops: 2011", James C, 2011
- Juliano BO, "The rice grain and its gross composition. In Rice Chemistry and Technology", pp.37~50 (1985), Juliano B.O. AACC, Inc.
- 이영택, "Variation and Correlation Analysis of Flavonoids and Carotenoids in Korean Pigmented Rice (Oryza sativa L.) Cultivars", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 58(24), pp.12804~12809, AMER CHEMICAL SOC
- 이현숙, "대규모 GMO 포장에서 비타민 A 강화 벼의 농업특성 검정", 한국육종학회지, 42(1), pp.56~60 (2010), 한국육종학회
- 오성덕, "해충저항성 Bacillus thuringiensis (Bt) 벼의 환경위해성 평가: 해충저항성 Bt벼가 물벼룩(Daphnia magna)에 미치는 영향", Journal of Applied Biological Chemistry, 54(4), pp.296~302 (2011), 한국응용생명화학회
- 우희종, "국내 유전자 변형 작물의 개발 현황", 한국국제농업개발학회지, 18(3), pp.221~229 (2006), 한국국제농업개발학회
- Ye X, "Engineering the provitamin A (β-carotene) biosynthetic pathway into (carotenoid-free) rice endosperm", Science, 5, pp.287~303 (2000)