Marker Assisted Development and Characterization of Beta-Carotene Rice
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Beta-carotene producing transformants were produced in the background of ‘Nagdongbyeo’, a Japonica rice cultivar. Introgression of the carotenoid locus in the transformant, PAC4-2 into the elite cultivar ‘Ilpumbyeo’ was started. To initiate a backcrossing program, we surveyed 220 SSR markers and found that 38% of them were polymorphic between ‘Ilpumbyeo’ as a recurrent parent and the PAC4-2 as a recipient parent. The selection strategy comprising foreground and background selection was employed. First, foreground selection was practiced in BC_1, BC_2, and BC_3 generations using the transgene specific PCR-based marker in addition to visual scoring of the seed color. Marker-based background selection combined with phenotypic selection was employed from BC_3F_2 to BC_3F_4 generations. Blast search indicated that the transgene PAC4-2 was located between SSR markers, RM6 and RM482. 240 BC_3F_3 and 63 BC_3F_4 lines were evaluated for four agronomic traits including days to heading. Most of the lines were similar to Ilpumbyeo in agronomic traits evaluated. The percentage of PAC4-2 genome ranged from 4% to 21% with a mean of 12.5%, which was higher than the expected for an unselected BC_3 backcross population. This could be explained by the fact that two genes for beta-carotene and the stripe virus resistance were targeted in this study. We selected 10 representative BC_3F_5 lines from 63 BC_3F_4 lines based on agronomic traits and carotenoids content. The selection strategy would be appropriate for the introgression of beta-carotene gene in a breeding program.
Abstract
Beta-carotene producing transformants were produced in the background of ‘Nagdongbyeo’, a Japonica rice cultivar. Introgression of the carotenoid locus in the transformant, PAC4-2 into the elite cultivar ‘Ilpumbyeo’ was started. To initiate a backcrossing program, we surveyed 220 SSR markers and found that 38% of them were polymorphic between ‘Ilpumbyeo’ as a recurrent parent and the PAC4-2 as a recipient parent. The selection strategy comprising foreground and background selection was employed. First, foreground selection was practiced in BC_1, BC_2, and BC_3 generations using the transgene specific PCR-based marker in addition to visual scoring of the seed color. Marker-based background selection combined with phenotypic selection was employed from BC_3F_2 to BC_3F_4 generations. Blast search indicated that the transgene PAC4-2 was located between SSR markers, RM6 and RM482. 240 BC_3F_3 and 63 BC_3F_4 lines were evaluated for four agronomic traits including days to heading. Most of the lines were similar to Ilpumbyeo in agronomic traits evaluated. The percentage of PAC4-2 genome ranged from 4% to 21% with a mean of 12.5%, which was higher than the expected for an unselected BC_3 backcross population. This could be explained by the fact that two genes for beta-carotene and the stripe virus resistance were targeted in this study. We selected 10 representative BC_3F_5 lines from 63 BC_3F_4 lines based on agronomic traits and carotenoids content. The selection strategy would be appropriate for the introgression of beta-carotene gene in a breeding program.
참고문헌 (21)
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