가뭄저항성벼(Agb0103)의 분자생물학적 특성과 안전성 평가
Molecular Biological Characteristics and Biosafety Assessment for Drought-tolerant Transgenic Rice (Agb0103)
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Genetically modified (GM) crops have been developed worldwide through the recombinant DNA technology andcommercialized by various agricultural biotechnology companies. Commercialization of GM crops will be required theassessment of risk associated with the release of GM crops. The purpose of this research is a molecular characterization ofintroduced T-DNA in transgenic rice T4∼T6 generation lines harboring a pepper MsrB2 gene under the control of stressinducible Rab21 promoter, as a part of biosafety evaluation for drought-tolerant transgenic rice (Agb0103). We identified thestructure and sequence of transformation vector of T-DNA and analyzed insertion sites, flanking sequences, and generationalstability of inserted T-DNA in transgenic rice lines. The transformation vector was consisted of right border, a drought-tolerantCaMsrB2 gene unit (Rab21 promoter:CaMsrB2:PinII terminator), a selectable marker herbicide resistance unit (CaMV 35Spromoter:bar:Nos terminator), and left border in sequential order. Based on the adaptor-ligation PCR and whole genomesequence database, we confirmed that T-DNA was introduced 2 copies (head to head type) at the position of 2,471,957∼2,472,049 bp of chromosome No. 8. From the generational stability study, T-DNAs were stably inherited through the T4 toT6 generations, and also stable expression of bar gene from T-DNA was confirmed. It was also confirmed that the backboneDNA of transformation vector containing antibacterial gene was not present in Agb0103 rice genome. These results will be filedto biosafety assessment document of Agb0103
Abstract
Genetically modified (GM) crops have been developed worldwide through the recombinant DNA technology andcommercialized by various agricultural biotechnology companies. Commercialization of GM crops will be required theassessment of risk associated with the release of GM crops. The purpose of this research is a molecular characterization ofintroduced T-DNA in transgenic rice T4∼T6 generation lines harboring a pepper MsrB2 gene under the control of stressinducible Rab21 promoter, as a part of biosafety evaluation for drought-tolerant transgenic rice (Agb0103). We identified thestructure and sequence of transformation vector of T-DNA and analyzed insertion sites, flanking sequences, and generationalstability of inserted T-DNA in transgenic rice lines. The transformation vector was consisted of right border, a drought-tolerantCaMsrB2 gene unit (Rab21 promoter:CaMsrB2:PinII terminator), a selectable marker herbicide resistance unit (CaMV 35Spromoter:bar:Nos terminator), and left border in sequential order. Based on the adaptor-ligation PCR and whole genomesequence database, we confirmed that T-DNA was introduced 2 copies (head to head type) at the position of 2,471,957∼2,472,049 bp of chromosome No. 8. From the generational stability study, T-DNAs were stably inherited through the T4 toT6 generations, and also stable expression of bar gene from T-DNA was confirmed. It was also confirmed that the backboneDNA of transformation vector containing antibacterial gene was not present in Agb0103 rice genome. These results will be filedto biosafety assessment document of Agb0103
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