비타민 A 강화벼 급이가 벼물바구미(Lissorhoptrus oryzophilus)의 살충제 감수성에 미치는 영향
Effect on Insecticide Susceptibility of Lissorhoptrus oryzophilus Fed on Carotenoid- Biofortified Rice Variety
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BACKGROUND: The carotenoid-biofortified (PAC) rice was generated by inserting phytoene synthase (Psy) and carotene desaturase (Crtl) genes isolated from Capsicum annuum cv. Nockwang and Pantoea ananatis into the genome of a conventional variety of rice (Nakdongbyeo). In our present study, we studied the effects on insecticide susceptibility of Rice Water Weevil (Lissorhoptrus oryzophilus). METHODS AND RESULTS: The L. oryzophilus were fed on carotenoid-biofortified (PAC) rice and its near non-genetically modified (GM) counterparts (Nakdongbyeo)under 25±1℃, humidity of 60±5%, and photoperiod 16L:8D for more than 60 days. Ten adults were soaked in the Clothianidin SC solution for 5 second in different concentrations,and were detected the mortalities after 24, 48 and 72 hours respectively. Every experiment was conducted with three replications. The cumulative mortalities of L. oryzophilus exposed to Clothianidin SC were similar between two types of feed administration. CONCLUSION: The results of this study suggested that carotenoid-biofortified rice might not affect the insecticide susceptibilities of Lissorhoptrus oryzophilus.
Abstract
BACKGROUND: The carotenoid-biofortified (PAC) rice was generated by inserting phytoene synthase (Psy) and carotene desaturase (Crtl) genes isolated from Capsicum annuum cv. Nockwang and Pantoea ananatis into the genome of a conventional variety of rice (Nakdongbyeo). In our present study, we studied the effects on insecticide susceptibility of Rice Water Weevil (Lissorhoptrus oryzophilus). METHODS AND RESULTS: The L. oryzophilus were fed on carotenoid-biofortified (PAC) rice and its near non-genetically modified (GM) counterparts (Nakdongbyeo)under 25±1℃, humidity of 60±5%, and photoperiod 16L:8D for more than 60 days. Ten adults were soaked in the Clothianidin SC solution for 5 second in different concentrations,and were detected the mortalities after 24, 48 and 72 hours respectively. Every experiment was conducted with three replications. The cumulative mortalities of L. oryzophilus exposed to Clothianidin SC were similar between two types of feed administration. CONCLUSION: The results of this study suggested that carotenoid-biofortified rice might not affect the insecticide susceptibilities of Lissorhoptrus oryzophilus.
참고문헌 (23)
- Bravo A, "Modeof action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control", Toxicon, 49, pp.423~435 (2007)
- Crickmore N., "Using worms to better understand how Bacillus thuringiensis kills insects", Trends Microbiology, 13, pp.347~350 (2005)
- Datta K., "Bioengineered “"golden”" indica rice cultivars with β-carotene metabolism in the endosperm with hygromycin and mannose selection systems", Plant Biotech J, 1, pp.81~90 (2003)
- De Vries J., "Microbial horizontal gene transfer and the DNA release from transgenic crop plants", Plant and Soil, 266, pp.91~104 (2004)
- FAO, "Recommended methods for the detection and measurement of resistance of agricultural pests to pesticides", Tentative methods for adults of the Colorado potato beetle, Leptinotarsa decemlineats (Say), Plant Protection Bulletin, 112~116 (1974)
- Ha S. H., "Application of two bicistronic systems involving 2A and IRES sequences to the biosynthesis of carotenoids in rice endosperm", PLANT BIOTECHNOLOGY JOURNAL, 8(8), pp.928~938 (2010), WILEY-BLACKWELL PUBLISHING
- Hoa T., "Golden Indica and Japonica rice lines amenableto deregulation", Plant Physiol, 133, pp.161~169 (2003)
- The global status of commercialized biotech, "GM crops: 2011", James C., 2011, ISAAA Briefs 43
- Juliano B.O., "The rice grain and its gross composition, In Rice Chemistry and Technology", pp.37~50 (1985), AACC, Inc
- 김금용, "물벼룩을 이용한 중금속 급성 독성 실험", 한국수처리학회지, 18(3), pp.37~47 (2010), 한국수처리학회
- Kim H.J., "Expression of PAT and NPT II proteins during the developmental stages of a genetically modified pepper developed in Korea", J Agric Food Chem, 58, pp.10906~10910 (2010)
- 이영택, "Variation and Correlation Analysis of Flavonoids and Carotenoids in Korean Pigmented Rice (Oryza sativa L.) Cultivars", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 58(24), pp.12804~12809, AMER CHEMICAL SOC
- 이찬원, "물벼룩을 이용한 농약의 급성 독성에 관한 연구", 한국환경과학회지, 16(1), pp.55~63 (2007), 한국환경과학회
- Nap J.P., "Biosafety of kanamycin-resistant transgenic plants", Transgenic Res, 1, pp.239~249 (1992)
- 오성덕, "해충저항성 Bacillus thuringiensis (Bt) 벼의 환경위해성 평가: 해충저항성 Bt벼가 물벼룩(Daphnia magna)에 미치는 영향", Journal of Applied Biological Chemistry, 54(4), pp.296~302 (2011), 한국응용생명화학회
- 오성덕, "해충저항성 Bt벼의 환경위해성 평가: 해충저항성 Bt벼가 미꾸리(Misgurnus anguillicaudatus) 및 잉어(Cyprinus carpio)에 미치는 영향", 한국국제농업개발학회지, 23(5), pp.570~577 (2011), 한국국제농업개발학회
- 오성덕, "해충저항성 Bacillus thuringiensis (Bt) 벼 급이가 벼물바구미(Lissorhoptrus oryzophilus)의 살충제 감수성에 미치는 영향", 한국국제농업개발학회지, 24(2), pp.247~253 (2012), 한국국제농업개발학회[DOI]
- Paine J.A., "Improving the nutritional value of golden rice through increased pro-vitamin A content", Nat Biotechnol, 23, pp.482~487 (2005)
- 손수인, "분자생물학적 분석을 통한 Bt 배추의 토양미생물상 영향 비교평가", 한국환경농학회지, 29(3), pp.293~299 (2010), 한국환경농학회
- Thomas Bøhn, "Demographic responses of Daphnia magna fed transgenic Bt-maize", Ecotoxicology, 19, pp.419~430 (2010)
- Versteeg D.J., "Ceriodaphnia and Daphnia: A comparison of their sensitivity to xenobiotics and utility as a test species", Chemosphere, 34, pp.869~892 (1997)
- 우희종, "국내 유전자 변형 작물의 개발 현황", 한국국제농업개발학회지, 18(3) (2006), 한국국제농업개발학회
- Ye X., "Engineering the provitamin A (β-carotene) biosynthetic pathway into (carotenoidfree) rice endosperm", Science, 5, pp.287~303 (2000)