레스베라트롤 합성 GM 벼 검정을 위한 계통특이 마커 개발
Development of Event-Specific PCR Marker for Identification of Transgenic Resveratrol-Enriched Rice Plant
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초록
최근 생명공학 기술의 발달로 국내에서도 다수의 형질전환작물들이 개발되어 상업화를 추진 중이다. 유전자변형 작물은상업화 이후에도 환경 및 주변작물에 대한 영향에 대한 지속적 평가가 필요하다. 본 연구는 국내에서 개발된 레스베라트롤 합성 벼 Agb0102의 관리 및 환경 모니터링을 위해 도입유전자와 삽입위치의 염기서열에 기초한 프라이머 조합을 이용한 PCR 증폭으로 Agb0102 계통 확인을 위한 계통특이 검정방법을 개발하였다. 본 연구에서 개발된 도입유전자 삽입부위의 계통 특이 프라이머를 사용한 다중 PCR 검정 방법은homozygous 및 heterozygous 상태의 Agb0102 벼와 일반품종의 구별이 쉽고 간편해 GM작물의 이동성 연구뿐 아니라 비의도적 오염에 대비한 사후 모니터링 및 형질전환 후대교배종 육성에도 유용하게 이용될 수 있을 것이다.
Abstract
A variety of genetically modified (GM) crops have been developed in Korea. In these crops, the resveratrol-enriched transgenic rice plant (Agb0102) has moved ahead to generate the dossier for regulatory review process required for commercialization of GM crop. The resveratrol-enriched transgenic rice plant could be released to farmers for cultivation after national regulators have determined that it is safe for the environment and human health. Here, we developed a PCR-based DNA marker based on flanking sequences of transgene for the discrimination of resveratrol-enriched transgenic rice plant. This DNA markers will be useful for identifying of resveratrol-enriched transgenic rice plant, and can also be used to estimate transgene movement occurred by pollen transfer or seed distribution. Moreover, it is helpful for prompt screening of a homozygote-transgenic progeny in the breeding program.
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