국내 승인 유전자변형 작물의 검출 기법 확립
Establishment of detection methods for approved LMO in Korea
2
조회
0
다운로드
5
인용
0
참고문헌
키워드
초록
AbstractLiving modified organisms (LMO) are one of the most widespread products of modern biotechnology after DNA discovery. Due to the decline of grain selfsufficiency rate and the increase of reliance on LMO imports in Korea, a series of concerns with regard to safety of living modified(LM) crops has been raised. The aim of this study is to establish the detection methods for unintentional release or growing of LMO plants in environmental conditions. To detect LM crop events, general concepts of specific primer design and PCR conditions were provided by the Joint Research Centre (JRC). The certified reference materials of seven LM events (4 soybean, 2 cotton and 1 corn) were obtained from the Institute for Reference Materials and Measurements (IRMM) and the American Oil Chemists’ Society (AOCS). Genomic DNA from seven LM events were purified and PCR amplifications were carried out by using individual event-specific primer sets. LM-specific PCR products of all seven events were efficiently amplified by our methods. The results indicate that the established detection method for LMOs is suitable as a scientific tool to monitor whether the crops found in natural environments are LMOs.
Abstract
AbstractLiving modified organisms (LMO) are one of the most widespread products of modern biotechnology after DNA discovery. Due to the decline of grain selfsufficiency rate and the increase of reliance on LMO imports in Korea, a series of concerns with regard to safety of living modified(LM) crops has been raised. The aim of this study is to establish the detection methods for unintentional release or growing of LMO plants in environmental conditions. To detect LM crop events, general concepts of specific primer design and PCR conditions were provided by the Joint Research Centre (JRC). The certified reference materials of seven LM events (4 soybean, 2 cotton and 1 corn) were obtained from the Institute for Reference Materials and Measurements (IRMM) and the American Oil Chemists’ Society (AOCS). Genomic DNA from seven LM events were purified and PCR amplifications were carried out by using individual event-specific primer sets. LM-specific PCR products of all seven events were efficiently amplified by our methods. The results indicate that the established detection method for LMOs is suitable as a scientific tool to monitor whether the crops found in natural environments are LMOs.
참고문헌 (22)
- Agbios, "Esential Biosafety", Merkvile, 1(1) (2001)
- "BIOSAFETY SCANNER"[URL]
- "Center for Environmental Risk Assessment"[URL]
- Event specific method for the quantification of Soybean MON87701 using real time PCR, "Charles Delobel C.", EUR, ~, pp.EUR25136EN.JRC 68071 (2011)
- Study on Environmental monitoring and post-management of LMO(Ⅵ), "Eom SH", NIE, ~, pp.NIE-2014-0039 (2013)
- "Joint Research Centre"[URL]
- "GMO Detection Method Database"[URL]
- "GM Approval Database"[URL]
- Establishment of LMO detection methods, "Kim TS", NIER, ~, pp.NIER-RP2011-1347 (2011)
- "Status of risk assessment of GMO in Korea"[URL]
- 이범규, "인천항만 주변 하천유역의 제초제 저항성 유전자변형유채 조사", Weed&Turfgrass Science, 27(1), pp.29~35 (2007), 한국잡초학회
- LMO detection methods, "Lee JR", ISBN, ~, pp.ISBN 979-11-86197-24-0 (2015)
- Event specific method for the quantification of hybrid cotton line 281-24-236, "3006-210-23 using real time PCR", Mazzara M, 2006, EUR22473EN
- Event specific method for the quantification of cotton GHB119 using real time PCR, "Mazzara M", Validation report and protocol, ~, pp.EUR22528EN.JRC 75655 (2012)
- "Self-sufficiency rate of grain in Korea"[URL]
- 박기웅, "국내 경작지에서의 수입 유전자변형작물 모니터링", Weed&Turfgrass Science, 27(4), pp.318~324 (2007), 한국잡초학회
- Event specific method for the quantification of Soybean MON87705 using real time PCR, "Savini C", Validation report and protocol, ~, pp.EUR25499EN.JRC 74376 (2012)
- Event specific method for the quantification of Maize MON87460 using real time PCR, "Savini C", Validation report and protocol, ~, pp.EUR25486EN.JRC 74374 (2012)
- Event specific method for the quantification of Soybean FG72 using real time PCR, "Savini C", Validation report and protocol, ~, pp.EUR254- 03EN.JRC 73091 (2012)
- Event specific method for the quantification of Soybean CV127 using real time PCR, "Savini C", Validation report and protocol, ~, pp.EUR- 25132EN.JRC 68070 (2011)
- Seralini GE, "Long term toxicity of a Roundup herbicide and a Roundup-tolerant genetically modified maize", Food and Chemical Toxicology, 50, pp.4221~4231 (2012)
- Establishment of LMO detection methods(Ⅱ), "Yoon JH", NIERRP, ~, pp.NIERRP2012-280 (2012)