국내 LMO 자연환경 모니터링을 위한 11개 LM 옥수수의 동시검출기법 개발
Four multiplex PCR Sets of 11 LM Maize for LMO environmental monitoring in Korea
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초록
유전자변형 옥수수의 개발과 상업적 이용이 증가하면서 유전자변형 옥수수 의심시료의 LMO 이벤트를 확인 할 수 있는 적합한 방법 개발이 필요하다. 국내에서는 상업적 재배와 자연생태계의 의도적·비의도적 LMO의 유출이 허용되고있지 않다. 본 연구에서는 국내 승인된 11개의 LM 옥수수 이벤트를 동시에 검출할 수 있는 동시검출기법을 개발하였다. 이 방법은 시간과 비용, 노동력을 절감할 수 있는 효율적인방법으로 4종의 PCR set로 개발하였다. 2012년부터 2014년까지 국립환경과학원 및 국립생태원에서 실시한 LMO 자연환경모니터링 의심시료를 이용하여 동시검출기법의 효율성을 검증하였다. 이러한 결과를 바탕으로 본 연구의 결과는 LMO 모니터링 시료의 분석에 적합한 방법임을 알 수 있었다.
Abstract
With the increasing development and commercial use of genetically modified maize, it is essential to develop an appropriate method for detection of individual LMO (Living modified organism) events for monitoring the samples. In South Korea, commercial planting and accidental or unintentional releases of LMOs into the environment were not approved. In this study, to increase the efficiency of LMO detection, we developed simultaneous detection methods for 11 LM maize events. This multiplex PCR detection method is economical, as it saves time, cost and labor. We developed 11 individual LM maize events, and applied 4 multiplex PCR sets to the LM maize samples. These results are confirmed by applying the multiplex analysis of LMO environmental monitoring from 2012 to 2014, which represents the unintentionally released LM maize samples. The data were correlated with event specific PCR results. Our results indicate that the multiplex PCR method developed is suitable for detection of LM maize in LMO monitoring.
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