통일형 벼 이앙 후 유묘기 재배안정성 증진을 위한 저온생장성 관련 QTL 탐색
Identification of QTLs Related to Plant Growth at Low Temperatures in the Seedling Stage of Tongil Type Rice after Transplanting
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In Asia, where climate change is increasing the damage caused by cold stress, it is crucial to cultivate varieties with enhanced cold tolerance. In this study, the Tongil variety ‘Hanareum2’ was crossed with the Japonica variety ‘Unkwang’ to improve plant growth ability at low temperatures during the seedling stage. This led to the development of 234 recombinant inbred line (RIL) populations, and a linkage map was constructed using 249 single nucleotide polymorphism (SNP) markers. The RIL populations were transplanted to the field one month earlier than the standard transplanting period, and plant height (PH), leaf number (LN), and dry weight (DW) were measured to identify quantitative trait loci (QTL) associated with plant growth ability at low temperatures during the seedling stage. QTLs related to cold tolerance, particularly those carrying the ‘Unkwang’ allele, were identified in the PH and DW traits. For PH, the QTLs qPH1, qPH5, and qPH8 were located on chromosomes 1, 5, and 8, respectively. Regarding DW, the QTLs qDW1, qDW8, and qDW9 were identified on chromosomes 1, 8, and 9. For the LN trait, qLN3 carrying the ‘Hanareum2’ allele was located on chromosome 3. If the identified QTLs are utilized, they can be incorporated into breeding programs for plant growth at low temperatures during the seedling stage.
Abstract
In Asia, where climate change is increasing the damage caused by cold stress, it is crucial to cultivate varieties with enhanced cold tolerance. In this study, the Tongil variety ‘Hanareum2’ was crossed with the Japonica variety ‘Unkwang’ to improve plant growth ability at low temperatures during the seedling stage. This led to the development of 234 recombinant inbred line (RIL) populations, and a linkage map was constructed using 249 single nucleotide polymorphism (SNP) markers. The RIL populations were transplanted to the field one month earlier than the standard transplanting period, and plant height (PH), leaf number (LN), and dry weight (DW) were measured to identify quantitative trait loci (QTL) associated with plant growth ability at low temperatures during the seedling stage. QTLs related to cold tolerance, particularly those carrying the ‘Unkwang’ allele, were identified in the PH and DW traits. For PH, the QTLs qPH1, qPH5, and qPH8 were located on chromosomes 1, 5, and 8, respectively. Regarding DW, the QTLs qDW1, qDW8, and qDW9 were identified on chromosomes 1, 8, and 9. For the LN trait, qLN3 carrying the ‘Hanareum2’ allele was located on chromosome 3. If the identified QTLs are utilized, they can be incorporated into breeding programs for plant growth at low temperatures during the seedling stage.
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