형광영상을 이용한 옥수수 반수체 종자 선발 효율성 향상
Improvement of Selection Efficiency of Haploid Maize Seeds Using Fluorescence Imaging
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초록
Many studies concerning breeding maize varieties are in progress in Korea and other countries. Double haploid technology is widelyused for the development of commercial maize varieties worldwide, and has also been utilized in Korea since its introduction by the MaizeResearch Institute, Gangwondo. We performed a study to improve the efficiency of selecting haploid maize seeds using fluorescence imaging. It was verified that anthocyanin produced by the expression of R1-nj gene can be detected by fluorescence imaging, and we developed ahigh-throughput method for discriminating between haploid and diploid seeds. Compared with discriminating with naked eye, this methodreduced the time for discriminating haploid and diploid maize by 91.7% and increased selection accuracy by 16.8% for haploid and 2.2%for diploid maize. This method enabled the acquisition of more haploid seeds and improved the efficiency of breeding research by shorteningthe time involved.
Abstract
Many studies concerning breeding maize varieties are in progress in Korea and other countries. Double haploid technology is widelyused for the development of commercial maize varieties worldwide, and has also been utilized in Korea since its introduction by the MaizeResearch Institute, Gangwondo. We performed a study to improve the efficiency of selecting haploid maize seeds using fluorescence imaging. It was verified that anthocyanin produced by the expression of R1-nj gene can be detected by fluorescence imaging, and we developed ahigh-throughput method for discriminating between haploid and diploid seeds. Compared with discriminating with naked eye, this methodreduced the time for discriminating haploid and diploid maize by 91.7% and increased selection accuracy by 16.8% for haploid and 2.2%for diploid maize. This method enabled the acquisition of more haploid seeds and improved the efficiency of breeding research by shorteningthe time involved.
참고문헌 (33)
- Altuntaş Y, "Identification of haploid and diploid maize seeds using convolutional neural networks and a transfer learning approach", Comput Electron Agric, 163, pp.104874~ (2019)
- Baleroni A, "Identification of haploids and diploids in maize using seedling traits and flow cytometry", Crop Breed Appl Biotechnol, 21, pp.e38422145~ (2021)
- Chaikam V, "Doubled haploid technology for line development in maize : technical advances and prospects", Theor Appl Genet, 132, pp.3227~3243 (2019)
- Chanoca A, "Imaging vacuolar anthocyanins with fluorescence lifetime microscopy(FLIM)", Methods Mol Biol, 1789, pp.131~141 (2018)
- Chase SS, "Monoploids and monoploid-derivatives of maize(Zea mays L. )", BOT REV, 35, pp.117~168 (1969)
- 최유미, "Differences in Cotyledon Color and Harvest Period Affect the Contents of Major Isoflavones and Anthocyanins in Black Soybeans", Plant Breeding and Biotechnology, 9(1), pp.65~76 (2021), 한국육종학회
- Coe EH, "The detection of haploids in maize", J Hered, 55, pp.231~233 (1964)
- Coe EH, "A line of maize with high haploid frequency", AM NAT, 93, pp.381~382 (1959)
- Collings DA, "Anthocyanin in the vacuole of red onion epidermal cells quenches other fluorescent molecules", Plants, 8, pp.596~ (2019)
- Cristiane T. Citadin, "Colorimetric and Chlorophyll Fluorescence Assays for Fast Detection and Selection of Transgenic Events of Cotton, Cowpea, Soybean and Common Bean Expressing the Atahas Gene", Plant Breeding and Biotechnology, 10(2), pp.94~101 (2022), 한국육종학회
- Edwards J, "The C1 locus in maize(Zea mays L. ) : effect on gene expression", Theor Appl Genet, 103, pp.718~724 (2001)
- Geiger HH, "Doubled haploids in hybrid maize breeding", Maydica, 54, pp.485~499 (2009)
- Girden ER, "ANOVA: Repeated measures" (1992), Sage
- Greenblatt IM, "A commercially desirable procedure for detection of monoploids in maize", J Hered, 58, pp.9~13 (1967)
- Irwin MG, "A commercially desirable procedure for detection of monoploids in maize", J Hered, 58, pp.9~13 (1967)
- 김영진, "반수체 밀 육성을 위한 밀 및 옥수수 품종 반응", 한국육종학회지, 46(3), pp.203~208 (2014), 한국육종학회[DOI]
- 이소명, "QTL Analysis for Fe and Zn Concentrations in Rice Grains Using a Doubled Haploid Population Derived from a Cross Between Rice (Oryza sativa) Cultivar 93-11 and Milyang 352", Plant Breeding and Biotechnology, 8(1), pp.69~76 (2020), 한국육종학회[DOI]
- Mackon E, "Subcellular localization and vesicular structures of anthocyanin pigmentation by fluorescence imaging of black rice (Oryza sativa L.) Stigma Protoplast", Plants, 10, pp.685~ (2021)
- Maqbool MA, "Doubled haploids in maize : development, deployment, and challenges", Crop Sci, 60, pp.2815~2840 (2020)
- Melchinger A, "Rapid and accurate identification of in vivo-induced haploid seeds based on oil content in maize", Sci Rep, 3, pp.2129~ (2013)
- Meng D, "Haploid induction and its application in maize breeding", Mol Breeding, 41, pp.20~ (2021)
- Nanda DK, "An embryo marker for detecting monoploids of maize(Zea Mays L. )", Crop Sci, 6, pp.213~215 (1966)
- Nowicka B, "Practical aspects of the measurements of nonphotochemical chlorophyll fluorescence quenching in green microalgae chlamydomonas reinhardtii using open FluorCam", Physiologia Plantarum, 168, pp.617~629 (2019)[DOI]
- Prasanna BM, "Doubled haploid technology in maize breeding: theory and practice", pp.1~8 (2012), CIMMYT
- Prigge V, "Production of haploids and doubled haploids in maize", Methods Mol Biol, 877, pp.161~172 (2012)
- Qu Y, "Improving the sorting efficiency of maize haploid kernels using an NMRbased method with oil content double thresholds", Plant Methods, 17, pp.2~ (2021)
- Ren S, "Monitoring the interaction between thermally induced whey protein and anthocyanin by fluorescence quenching spectroscopy", Foods, 10, pp.310~319 (2021)
- Rotarenco V, "New inducers of maternal haploids in maize", Maize Genet Coop News Lett, 84, pp.21~22 (2010)
- Tallarida RJ, "Manual of pharmacologic calculations", pp.145~148 (1987), Springer
- Tukey JW, "Exploratory data analysis" (1977), Pearson
- Wang N, "Haploid induction by a maize cenh3 null mutant", Sci Adv, 7, pp.eabe2299~ (2021)
- Wu MF, "I am all ears : maximize maize doubled haploid success by promoting axillary branch elongation", Plant Direct, 4, pp.1~8 (2020)
- Zhang R, "Handheld multifunctional fluorescence imager for non-invasive plant phenotyping", Front Plant Sci, 13, pp.822634~ (2022)