c-Fos Immunoreactivity in the Neurons of the Lateral Geniculate Nucleus in Albino Rats by Light Exposure after Dark Rearing
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Purpose: To investigate the effect of dark rearing immediately after birth on the maturation of the visual relay neurons in the lateral geniculate nucleus. Methods: Fifty neonatal rats were used. Neonates of the control groups were raised under a normal light/dark cycle. Neonates of the experiment groups were dark reared and isolated from light during the entire experimental period,then exposed to the sun light for 1 hour before sacrifice. Results: In the control groups, the neurons in the dorsal lateral geniculate nucleus developed normally at each age tested. In the experiment groups, the cytoplasm of the large neurons in the dorsal lateral geniculate nucleus of 2-week-old rats contained small vesicles, and the cytoplasm of the large neurons of 4-week-old rats was converted into a vacuole-like space. Moreover, c-Fos immunoreactivity of the large neurons in the dorsal lateral geniculate nucleus in the experiment groups was significantly increased compared to that of the control groups. Conclusions: We suppose that the maturation of the neurons in the lateral geniculate nucleus might be influenced by light stimulation during the critical period. Furthermore, c-Fos could be a marker of the functional activity of the visual relay neurons of the lateral geniculate nucleus in albino rats.
Abstract
Purpose: To investigate the effect of dark rearing immediately after birth on the maturation of the visual relay neurons in the lateral geniculate nucleus. Methods: Fifty neonatal rats were used. Neonates of the control groups were raised under a normal light/dark cycle. Neonates of the experiment groups were dark reared and isolated from light during the entire experimental period,then exposed to the sun light for 1 hour before sacrifice. Results: In the control groups, the neurons in the dorsal lateral geniculate nucleus developed normally at each age tested. In the experiment groups, the cytoplasm of the large neurons in the dorsal lateral geniculate nucleus of 2-week-old rats contained small vesicles, and the cytoplasm of the large neurons of 4-week-old rats was converted into a vacuole-like space. Moreover, c-Fos immunoreactivity of the large neurons in the dorsal lateral geniculate nucleus in the experiment groups was significantly increased compared to that of the control groups. Conclusions: We suppose that the maturation of the neurons in the lateral geniculate nucleus might be influenced by light stimulation during the critical period. Furthermore, c-Fos could be a marker of the functional activity of the visual relay neurons of the lateral geniculate nucleus in albino rats.
참고문헌 (21)
- Morgan JI, "Stimulus-transcription coupling in the nervous system:involvement of the inducible proto-oncogenes fos and jun", Annu Rev Neurosci, 14, pp.421~451 (1991)
- Greenberg ME, "Nerve growth factor and epidermal growth factor induce rapid transient changes in proto-oncogene transcription in PC12 cells", J Biol Chem, 260, pp.14101~4110 (1985)
- Hamamura M, "Differential decreases in c-fos and aldolase C mRNA expression in the rat cerebellum after repeated administration of methamphetamine", Brain Res Mol Brain Res, 64, pp.119~131 (1999)
- Imaki T, "Intracerebroventricular administration of corticotropin-releasing factor induces c-fos mRNA expression in brain regions related to stress responses:comparison with pattern of c-fos mRNA induction after stress", Brain Res, 616, pp.114~125 (1993)
- Chaudhuri A, "Molecular maps of neural activity and quiescence", Acta Neurobiol Exp(Wars), 60, pp.403~410 (2000)
- Yoshida K, "Differential expression of c-fos mRNA in rat retinal cells:regulation by light", dark cycle, 1993, pp.10, 1049~1054
- Lu B, "Increased c-fos-like immunoreactivity in the superior colliculus and lateral geniculate nucleus of the rd mouse", Brain Res, 1025, pp.220~225 (2004)
- Mower GD, "Fos expression during the critical period in visual cortex:differences between normal and dark reared cats", Brain Res Mol Brain Res, 64, pp.264~269 (1999)
- Wang WF, "Glucose metabolism in the visual structures of rat monocularly deprived by eyelid suture after postnatal eye opening", Jpn J Ophthalmol, 49, pp.6~11 (2005)
- Cynader M, "Prolonged sensitivity to monocular deprivation in dark-reared cats", J Neurophysiol, 43, pp.1026~1040 (1980)
- Mower GD, "Immediate early gene expression in the visual cortex of normal and dark reared cats:differences between fos and egr-1", Brain Res Mol Brain Res, 105, pp.157~160 (2002)
- Rosen KM, "Brief visual experience induces immediate early gene expression in the cat visual cortex", Proc Natl Acad Sci U S A, 89, pp.5437~5441 (1992)
- Fosse VM, "Postnatal development of glutamatergic,GABAergic,and cholinergic neurotransmitter phenotypes in the visual cortex,lateral geniculate nucleus,pulvinar,and superior colliculus in cats", J Neurosci, 9, pp.426~435 (1989)
- Piechaczyk M, "c-Fos proto-oncogene regulation and function", Crit Rev Oncol Hematol, 17, pp.93~131 (1994)
- Shaulian E, "AP-1 in cell proliferation and survival", Oncogene, 20, pp.2390~2400 (2001)
- Alitalo R, "Increased erythroid potentiating activity", tissue inhibitor of metalloproteinases and jun, Blood, pp.1990, 75[DOI]
- Caubet JF, "Expression of the c-fos protooncogene by human and murine erythroblasts", Blood, 74, pp.947~951 (1989)
- Dony C, "Proto-oncogene c-fos expression in growth regions of fetal bone and mesodermal web tissue", Nature, 328, pp.711~714 (1987)
- Smeyne RJ, "Temporal and spatial expression of a fos-lacZ transgene in the developing nervous system", Brain Res Mol Brain Res, 16, pp.158~162 (1992)
- Olson CR, "Profile of the sensitive period for monocular deprivation in kittens", Exp Brain Res, 39, pp.17~21 (1980)
- Ohki K, "c-fos gene expression in postnatal rat retinas with light", dark cycle, 1996, pp.36, 1883~1886