흰쥐 안구적출에 따른 위둔덕 calretinin발현의 변화
Changes of Calretinin-Immunoreactivities in the Rat SuperiorCollicuclus after Eye Enucleation
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간추림 : 위둔덕얕은층은 망막, 시각겉질, 뇌줄기 신경핵 등에서 오는 들신경섬유들을 받아들여 시각자극에 대한반응, 습관적인 행동, 유해자극에 대한 반사 등에도 관련이 있는 것으로 밝혀지고 있다. 어린 흰쥐의 한쪽 안구적출에 따라 나타나는 위둔덕얕은층의 변화를 알아보기 위해 망막-위둔덕 신경추적자와 칼슘결합단백질의 하나인 calretinin의 발현 양상을 면역조직화학염색을 실시하였다. 출생 직후 흰쥐의 망막-위둔덕 투사섬유들은 대부분 반대쪽 위둔덕얕은층에 도달하는 것을 알 수 있었다. 안구적출쪽 위둔덕의 calretinin 발현은 calretinin양성세포들이 띠층, 얕은회색층, 시신경층 등에 산재해 있었고, calretinin양성신경섬유들이 위둔덕얕은층에서 진하게 염색되는 양상을 보였다. 안구적출 반대쪽 위둔덕얕은층의calretinin양성세포들은 적출쪽에 비해 현저히 증가하였고, calretinin양성섬유들의 염색성은 감소하였다.안구적출에 의해 망막-위둔덕 경로가 차단됨에 따라 위둔덕얕은층의 calretinin양성세포들의 증가와 calretinin 양성섬유의 감소가 나타나는데, 이는 위둔덕신경세포들의 재구축과 관련된 것으로 보인다.
Abstract
Calcium-binding proteins play an important role in the protection, differentiation, and reorganization of the central nervous system. The effects of neonatal retina deafferentiation on calretinin, and tracing of retinotectal pathway were examined immunohistochemically in the superficial layer of the rat superior colliculus. Tracing with cholera toxin was revealed on the superior colliculus contralateral to the ocular injection. On the contralateral side of superior colliculus, the calretinin- immunoreactive (IR) cells were dramatically increased, calretinin-IR fibers were markedly decreased in the superficial layer. These results show that retinal deafferentation results in an increase of calretinin-immunoreactive cells within the superficial layers of the superior colliculus, which suggest reorganization of neurons in superior colliculus.
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