Distribution of Interleukin-22–secreting Immune Cells in Conjunctival Associated Lymphoid Tissue
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Interleukin (IL)-22 is a cytokine involved in epithelial cell regeneration. Currently, no research studieshave analyzed the distribution of the three distinct IL-22–secreting cell populations in human or mouse conjunctiva. This study investigated the distribution of the three main populations of IL-22–secreting immune cells, αβ Thcells, γδ T cells, or innate cells (innate lymphoid cells [ILCs] or natural killer cells), in conjunctival associated lymphoidtissues (CALTs) in human and mouse models. Methods: We collected discarded cadaveric bulbar conjunctival tissue specimens after preservation of the corneo-limbal tissue for keratoplasty from four enucleated eyes of the domestic donor. The bulbar conjunctivatissue, including the cornea from normal (n = 27) or abraded (n = 4) B6 mice, were excised and pooled in RPMI1640 media. After the lymphoid cells were gated in forward and side scattering, the αβ Th cells, γδ T cells, orinnate lymphoid cells were positively or negatively gated using anti-CD3, anti-γδ TCR, and anti–IL-22 antibodies,with a FACSCanto flow cytometer. Results: In normal human conjunctiva, the percentage and number of cells were highest in αβ Th cells, followed byγδ T cells and CD3– γδ TCR – IL-22+ innate cells (presumed ILCs, pILCs) (Kruskal-Wallis test, p = 0.012). In normalmice keratoconjunctiva, the percentage and total number were highest in γδ T cells, followed by αβ Th cellsand pILCs (Kruskal-Wallis test, p = 0.0004); in corneal abraded mice, the population of αβ Th cells and pILCstended to increase. Conclusions: This study suggests that three distinctive populations of IL-22–secreting immune cells are presentin CALTs of both humans and mice, and the proportions of IL-22+αβ Th cells, γδ T cells, and pILCs in CALTs inhumans might be differently distributed from those in normal mice.
Abstract
Interleukin (IL)-22 is a cytokine involved in epithelial cell regeneration. Currently, no research studieshave analyzed the distribution of the three distinct IL-22–secreting cell populations in human or mouse conjunctiva. This study investigated the distribution of the three main populations of IL-22–secreting immune cells, αβ Thcells, γδ T cells, or innate cells (innate lymphoid cells [ILCs] or natural killer cells), in conjunctival associated lymphoidtissues (CALTs) in human and mouse models. Methods: We collected discarded cadaveric bulbar conjunctival tissue specimens after preservation of the corneo-limbal tissue for keratoplasty from four enucleated eyes of the domestic donor. The bulbar conjunctivatissue, including the cornea from normal (n = 27) or abraded (n = 4) B6 mice, were excised and pooled in RPMI1640 media. After the lymphoid cells were gated in forward and side scattering, the αβ Th cells, γδ T cells, orinnate lymphoid cells were positively or negatively gated using anti-CD3, anti-γδ TCR, and anti–IL-22 antibodies,with a FACSCanto flow cytometer. Results: In normal human conjunctiva, the percentage and number of cells were highest in αβ Th cells, followed byγδ T cells and CD3– γδ TCR – IL-22+ innate cells (presumed ILCs, pILCs) (Kruskal-Wallis test, p = 0.012). In normalmice keratoconjunctiva, the percentage and total number were highest in γδ T cells, followed by αβ Th cellsand pILCs (Kruskal-Wallis test, p = 0.0004); in corneal abraded mice, the population of αβ Th cells and pILCstended to increase. Conclusions: This study suggests that three distinctive populations of IL-22–secreting immune cells are presentin CALTs of both humans and mice, and the proportions of IL-22+αβ Th cells, γδ T cells, and pILCs in CALTs inhumans might be differently distributed from those in normal mice.
참고문헌 (13)
- Nikoopour E, "IL-22, cell regeneration and autoimmunity", Cytokine, 74, pp.35~42 (2015)[DOI]
- Zenewicz LA, "Recent advances in IL-22 biology", Int Immunol, 23, pp.159~163 (2011)[DOI]
- Huang Y, "<TEX>${\gamma}{\delta}$<", TEX> T cell-dependent regulatory T cells prevent the development of autoimmune keratitis, 2015, pp.195, 5572~5581
- Li Z, "CCL20, <TEX>${\gamma}{\delta}$<", TEX> T cells, and IL-22 in corneal epithelial healing, 2011, pp.25, 2659~2668
- Li Z, "Gamma delta T cells are necessary for platelet and neutrophil accumulation in limbal vessels and efficient epithelial repair after corneal abrasion", Am J Pathol, 171, pp.838~845 (2007)[DOI]
- Liu Q, "NK cells modulate the inflammatory response to corneal epithelial abrasion and thereby support wound healing", Am J Pathol, 181, pp.452~462 (2012)[DOI]
- Sedda S, "An overview of the role of innate lymphoid cells in gut infections and inflammation", Mediators Inflamm, 2014, pp.235460~ (2014)
- Goto Y, "Innate lymphoid cells regulate intestinal epithelial cell glycosylation", Science, 345, pp.1254009~ (2014)[DOI]
- Dudakov JA, "Interleukin-22: immunobiology and pathology", Annu Rev Immunol, 33, pp.747~785 (2015)[DOI]
- Ji YW, "Lacrimal gland-derived IL-22 regulates IL-17-mediated ocular mucosal inflammation", Mucosal Immunol, 10, pp.1202~1210 (2017)[DOI]
- O'Brien RL, "Protective role of gammadelta T cells in spontaneous ocular inflammation", Invest Ophthalmol Vis Sci, 50, pp.3266~3274 (2009)[DOI]
- Misharin AV, "Flow cytometric analysis of macrophages and dendritic cell subsets in the mouse lung", Am J Respir Cell Mol Biol, 49, pp.503~510 (2013)[DOI]
- Montaldo E, "Human innate lymphoid cells", Immunol Lett, 179, pp.2~8 (2016)[DOI]