주파수별 전기침이 카라기난을 주사한 휘주 척수의 c-fos 및 수축촉진 글루탐산염 수용기 발현에 미치는 영향
Effects of electroacupuncture with different frequencies on the spinal c-fos and inotropic glutamate receptor expression in carrageenan-injected rat
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간추림 : 흰쥐 오른쪽 발바닥에 1% 카라기난(carrageenan)을 주사한 후 2 Hz, 15 Hz 및 120 Hz 전기침을 30분간 자 극하였을 때 척수내 c-fos 및 수축촉진 글루탐산염 수용기(inotropic glutamate receptor) 발현에 미치는 영향을 면 역조직화학적으로 검색하였다. 카라기난 주사 후 척수내 c-fos 발현은 정상군에 비해 현저히 증가하나 전기침군은 카라기난을 주사한 대조군에 비해 감소하였으며 특히 2Hz와 15 Hz 전기침군에서 유의성 있는 감소를 보여 주었 으며 2 ㎐ 전기침군의 감소가 더욱 현저하였다. 수축촉진 글루탐산염 수용기의 면역반응은 주로 척수 뒤뿔 얕은 층판에서 강한 면역반응을 보였으며 정상군에 비해 대조군에서 모두 증가하였다. N-methyl-D-aspartate (NMDA) 수용기인 NR-1과 NR-2A 면역반응을 살펴보면 두 면역반응 모두 대조군에 비해 전기침군에서 감소하였으나 NR-1은 유의성은 보이지 않았고, NR-2A 면역반응은 2 Hz 전기침군에서 유의성 있게 감소하였다. α-amino-3- hydroxy-5-methy-4-isoxazolepropionic acid (AMPA) 수용기의 GluR-1 면역반응은 대조군에 비해 2 Hz와 120 Hz 전기침군에서 유의성 있게 증가한데 비해 GluR-2/3 면역반응은 유의성 있게 감소하였다. 주파수별 전기침 작 용은 c-fos 면역반응으로 보아 2 Hz 전기침군의 변화가 가장 현저하였으며 NMDA 수용기발현은 저주파수인 2Hz 전기침에서, AMPA 수용기발현은 15 Hz와 고주파수인 120 Hz 전기침군에서 변화를 보여 전기침 작용에 있어 이들 수용기가 관여함을 알 수 있다.
Abstract
We investigated the comparative effects of electroacupuncture (EA) with different frequency on the spinal c-fos and inotropic glutamate receptor expression in carrageenan-injected rats. Bilateral EA stimulation with 2, 15 and 120 Hz were delivered at those acupoints corresponding to Zusanli and Sanyinjiao in man via the needles. The inhibitory effects of the EA on the inflammatory process were investigated in the central nociceptive sites by immunohistochemical analysis. Three hours after carrageenan injection, the number of c-fos-like immunoreactive (LI) neurons was significantly increased in all layers of the ipsilateral spinal cord at L4-5 segment. But these immunoreactive neurons were markedly reduced in the spinal gray matter, especially in the superficial laminae, by all kinds of EA stimulation. The number of NMDA(NR-1 and NR-2A) and AMPA receptors (GluR-1, GluR-2/3)-LI neurons was also increased by carrageenan injection. But NR-2A-LI neuron was significantly reduced in superficial laminae of dorsal horn by 2 Hz EA stimulation. While GluR-1-LI neuron of 15 Hz and 120 Hz EA was increased in the nucleus proprius compared with carrageenan- injected group, GluR-2/3-LI neuron of these EA stimulated groups significantly reduced in superficial laminae of dorsal horn. In conclusion, EA treatment can attenuate spinal c-fos expression in carrageenan injected rats and regulates spinal inotropic glutamate receptor expression.
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