Complete Freund’s adjuvant 유발 관절염 모델 흰쥐의 척수 뒤뿔내 neuronal nitric oxide synthase 발현에 대한 전기침의 영향
Effects of Electroacupuncture on the Expression of Spinal Neuronal Nitric Oxide Synthase in Adjuvant-induced Arthritic Rat Model
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초록
전기침의 항부종 및 통각과민에 대한 효과와 척수내 nitric oxide (NO)의 관련성을 알아보기 위해 complete Freund’s adjuvant (CFA)주사한 만성 관절염 모델을 사용하여 척수 뒤뿔내 neuronal nitric oxide synthase (nNOS)의 발현을 살펴 보았다. 흰쥐 오른쪽 발바닥에 CFA 주사한 후 족삼리와 삼음교에 해당하는 혈위를 2 Hz, 15 Hz 및 120 Hz 전기침을 30 분간 3일 간격으로 30일 동안 처치하였다. CFA 주사 후 30일에 척수 중 제1번, 제3번 및 제5번 허리분절에 해당 하는 분절의 nNOS발현을 면역조직화학적으로 검색하였다. CFA를 주사한 흰쥐에서 척수 뒤뿔의 모든 부위에서 nNOS 발현이 현저히 증가하였으며 특히 superficial laminae (SDH, laminae I and II)에서의 증가가 현저하였다. 그러나 모든 전기침군에서 nNOS의 발현이 감소하였는 데 특히 제1번과 제3번 허리분절의 SDH와 nucleus proprius (NP, laminae III and IV)부위에서 모든 전기침군이, neck of the dorsal horn (NECK, laminae V and VI)부위에서 2Hz 전기침군이 유의성 있는 감소를 보여 주었다. 제5 번 허리분절에서 NP와 NECK부위에서 2Hz와 15 Hz 전기침이 유의성 있는 효과를 보여 주었다. 이로 보아 전기침은 척수 뒤뿔내 nNOS발현억제를 통한 통각과민 완화 효과를 나타내는 것을 알 수 있으며 통 각유발과 NO관련성을 유추할 수 있으며 전기침의 항염증의 효과 검정에 있어 nNOS발현이 주요 표지로 이용가능 할 것이다.
Abstract
To examine the effects of electroacupuncture (EA) on central nociceptive modulation, expressional changes of spinal neuronal nitric oxide synthase (nNOS) were investigated in complete Freund’s adjuvant (CFA)-injected rats. Inflammation was induced by an intraplantar injection of CFA into the hindpaw of male Sprague-Dawley rats. Bilateral EA stimulation at 2 Hz, 15 Hz and 120 Hz was applied at those acupoints corresponding to Zusanli and Sanyinjiao in man with 3-day intervals for 30 days. At 30 days after CFA-injection, effects of EA on nNOS expression were observed in the dorsal horn of the spinal cord using immunohistochemical methods. The mean integrated optical density of nNOS immunoreaction was significantly increased in the dorsal horn throughout L1 to L5 lumbar segments in CFA-injected rats. The nNOS expression was attenuated in all regions of the dorsal horn by all types of EA. Especially, these reaction was markedly decreased in the superficial laminae and nucleus proprius of L1 and L3 lumbar segments by three types of EA, but a marked decrease in the neck of the dorsal horn was observed only in 2 Hz stimulation. The marked decrease of nNOS also showed in nucleus proprius and the neck of L5 lumbar segments in 2 Hz and 15 Hz EA stimulated rats. It is concluded that EA treatment can attenuate chronic inflammatory process in CFA-injected rats through modulating expression of nNOS in the dorsal horn of the spinal cord.
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