생쥐 치아 발생과정 중 치아상피에서 Uchl1의 발현
Expression of Uchl1 in the Dental Epithelium during Mouse Tooth Development
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초록
치아는 발생과정에서 외배엽과 신경능으로부터 유래되는 중간엽의 상호유도작용에 의하여 형성되는 것 으로 알려져 있다. 현재까지 치아의 형태형성과 분화과정에서 신경인자가 관여하는 지에 대해서는 분명하지 않다. 본 연구에서는 치아 발생과정에서 신경인자의 역할을 구명하기 위하여, 생쥐 치배를 대상으로 in situ hybridization과 면역조직화학법을 이용하여 Uchl1의 발현 및 분포양상을 확인하였다. 싹시기의 치배에서 Uchl1의 전사체는 치판과 구강상피에서 약하게 발현되었으나 모자시기의 치배에서는 구강 상피에서는 발현되지 않았으며 안쪽치아상피와 별모양그믈, 치유두에서 제한적으로 발현되었다. 종시기부터 Uchl1 은 안쪽치아상피와 법랑모세포에서 발현되었다. Uchl1는 신생 생쥐의 앞니와 어금니의 안쪽 치아상피와 분화하는 법랑모세포에서 분포하고 있었다. 생후 5일된 생쥐의 어금니에서 Uchl1은 완전히 분화된 법랑모세포와 유두층에 서 매우 강하게 발현되는 반면에 상아모세포에서는 약하게 발현되었다. 이상의 결과로 보아 Uchl1은 치아 발생과 정에서 분화하는 치아상피와 법랑모세포에서 발현되고 분포함이 확인되었다. 따라서 신경유래 단백질인 Uchl1은 비신경성 치아상피의 조직분화와 세포분화 과정에서 중요한 역할을 수행할 것임을 제시한다.
Abstract
Tooth is formed by the reciprocal interactions between the ectoderm and ectomesenchyme derived from neural crest. It has not been clear that neuronal factors involved in the morphogenesis and differentiation of tooth. To identify the roles of neuronal factors during the tooth development, the expression patterns and localization of Uchl1 were investigated in the developing mouse tooth germ by in situ hybridization and immunohistochemistry. Uchl1 transcripts were weakly expressed in the oral epithelium and dental lamina at bud stage. However, expression of Uchl1 was not found in the oral epithelium from cap stage and observed in the inner enamel epithelium, stellate reticulum and dental papilla. From the bell stage, Uchl1 was expressed in the inner enamel epithelium and ameloblasts. Uchl1, was appeared to be localized in the inner enamel epithelium and differentiating ameloblasts of molar and incisors at neonates. Uchl1 was localized strongly in the fully differentiated ameloblasts and adjacent papillary layer whereas localized weakly in the odontoblasts of the molar at postnatal day 5. From these results, Uchl1 was expressed and localized in the differentiating dental epithelium and ameloblasts during tooth development. The results suggest that neuronal protein, Uchl1 may play roles in the histo- and cyto-differentiation of non-neuronal dental epithelium.
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