흰쥐 난포에서 저선량 방사선조사로 유도된 hormesis효과
Low-dose Radiation-induced Hormetic Effect in the Rat Ovarian Follicle
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초록
Hormesis효과는 생물체가 자연속에 존재하는 정도의 저선량 방사선 또는 독성물질 등에 노출된 후 단기 간 내에 나타나는 이로운 영향을 말한다. 저자들은 흰쥐 난포에서 저선량의 방사선을 조사한 후 고선량을 조사하 여 hormesis효과를 알아보고자 하였다. 본 실험에서는 4주령의 암컷 흰쥐(Sprague-Dawley계통)를 대조군, 2 cGy조사군, 2 cGy 조사후 2Gy조사군 및 2 Gy조사군으로 나누고, 각 군별로 단회 방사선 조사후 24시간에 희생시켜 난소를 적출하고 통상적인 방법으로 처 리한 후 H & E 염색과 TUNEL 면역염색을 실시하였다. 광학현미경으로 각 군 사이에 폐쇄난포의 비율과 난포의 발육단계별 정상난포의 비율을 비교하였다. 그 결과 난소내 전체 난포에서 차지하는 폐쇄난포의 비율은 2 Gy조사군(55.35 %)에서 2 cGy+2 Gy조사군 (39.27 %)에 비하여 현저히 증가하였으며, 난포의 발육단계에 따른 정상난포의 비율은 2 Gy조사군(일차난포, 82.15 %; 난포동형성전난포, 63.48 %; 난포동 난포, 21.58 %) 보다 2 cGy+2Gy조사군(일차난포, 94.37 %; 난포동형 성전난포, 78.81 %; 난포동 난포, 33.09 %)에서 모든 발육단계에서 유의하게 증가 하였다. 이같은 결과는 저선량의 방사선에 의해 난포발육단계에 hormesis효과가 나타났음을 의미하는 것으로 앞으로 방사선 hormesis연구에 있어 한 모델로 사용할 수 있을 것으로 사료된다.
Abstract
Hormesis is the generally-favorable biological responses to low exposures to toxins and other stressors. Radiation hormesis is the theory that ionizing radiation is benign at low levels of exposure, and that doses at the level of natural background radiation can be beneficial. The purpose of this study is to reveal the hormetic effect of low-dose radiation of ionizing radiation on the ovarian follicles of 4-week old female mice. Mice were grouped into control group, 2 cGy irradiated group, 2 cGy and 2 Gy irradiated group (2 cGY pre-exposure group), and 2 Gy irradited group. Mice were sacrificed by cervical dislocation 24 hours after irradiation, removed ovaries, fixed in neutral formaldehyde solution for 24 hours, embedded with paraffin, stained with hematoxylin and eosin and TUNEL immunohistochemically, and observed light microscopically the atretic follicles and normal follicles in various follicular developmental stages. In this experiment, the ratrio of atretic follicles to entire follicles in an ovary increased significantly in 2 Gyirradiated group compared with 2 cGY pre-exposure group, and the ratio of normal follicles to the entire follicles in an ovary in all the developmental stages were increased significantly in the 2 cGY pre-exposure group compared with 2 Gy-irradiated group. These results mean that low-dose radiation pre-exposure can induce the hormetic effect in the developing ovarian follicle.
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