Expression of Fas-associated Factor 1 in the Developing Testis
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
FAS associated factor 1 (FAF1) is a Fas-associating molecule, which enhances Fas mediated apoptosis. FAF1 gene is expressed most abundantly in the testis among the mouse organs. The aim of this study was to reveal the expression and the role of FAF1 in the developing testis. H-E stain and FAF1 immunohistochemistry were performed in the testis and epididymis of the E15.5 embryo, and 1, 2, and 8 week-old C57/BL6 mice. FAF1 was expressed in the testis from E 15.5 embryo to 8 week-old mice. Cell type of FAF1 positive cells was different among the developmental stage. Furthermore, cellular (cytoplasmic or nuclear) localization of FAF1 in the male germ cells was different during the developmental stage. FAF1 was expressed mainly in the nuclei of the germ cells 1 and 8 weeks after birth, when cell differentiation occurs actively in the testis. However, FAF1 was expressed in the cytoplasms of germ cells 2 weeks after birth, when apoptosis occurs maximally in the testis. Taken together, it can be suggested FAF1 expressed in male germ cells in the testis. FAF1 might be involved in regulation of the cellular function during spermatogenic cell differentiation and apoptosis in the testis.
Abstract
FAS associated factor 1 (FAF1) is a Fas-associating molecule, which enhances Fas mediated apoptosis. FAF1 gene is expressed most abundantly in the testis among the mouse organs. The aim of this study was to reveal the expression and the role of FAF1 in the developing testis. H-E stain and FAF1 immunohistochemistry were performed in the testis and epididymis of the E15.5 embryo, and 1, 2, and 8 week-old C57/BL6 mice. FAF1 was expressed in the testis from E 15.5 embryo to 8 week-old mice. Cell type of FAF1 positive cells was different among the developmental stage. Furthermore, cellular (cytoplasmic or nuclear) localization of FAF1 in the male germ cells was different during the developmental stage. FAF1 was expressed mainly in the nuclei of the germ cells 1 and 8 weeks after birth, when cell differentiation occurs actively in the testis. However, FAF1 was expressed in the cytoplasms of germ cells 2 weeks after birth, when apoptosis occurs maximally in the testis. Taken together, it can be suggested FAF1 expressed in male germ cells in the testis. FAF1 might be involved in regulation of the cellular function during spermatogenic cell differentiation and apoptosis in the testis.
참고문헌 (25)
- Adham IM, "Fas-associated factor (FAF1) is required for the early cleavage-stages of mouse embryo", Mol Hum Reprod, 14, pp.207~213 (2008)
- Allan DJ, "Spermatogonial apoptosis has three morphologically recognizable phases and shows no circadian rhythm during normal spermatogenesis in the rat", Cell Prolif, 25, pp.241~250 (1992)
- Becker K, "Interaction of Fas (Apo-1", CD95) with proteins implicated in the ubiquitination pathway, 1997, pp.412, 102~106
- Billig H, "Apoptosis in testis germ cells: developmental changes in gonadotropin dependence and localization to selective tubule stages", Endocrinology, 136, pp.5~12 (1995)
- Boulogne B, "Apoptosis and mitosis in gonocytes of the rat testis during foetal and neonatal development", Int J Androl, 22, pp.356~365 (1999)
- Brinkworth MH, "Identification of male germ cells undergoing apoptosis in adult rats", J Reprod Fertil, 105, pp.25~33 (1995)
- Chu K, "A Fas-associated protein factor, FAF1, potentiates Fas-mediated apoptosis", Proc Natl Acad Sci U S A, 통권 : 92, pp.11894~11898 (1995)
- Cooke HJ, "Mouse models of male infertility", Nat Rev Genet, 3, pp.790~801 (2002)
- de Rooij DG, "Proliferation and differentiation of spermatogonial stem cells", Reproduction, 121, pp.347~354 (2001)
- Frohlich T, "Characterization of novel nuclear targeting and apoptosis-inducing domains in FAS associated factor 1", J Cell Sci, 111, pp.2353~2363 (1998)
- Ginsburg M, "Primordial germ cells in the mouse embryo during gastrulation", Development, 110, pp.521~528 (1990)
- Kim HJ, "Human Fasassociated factor 1 interacts with heat shock protein 70 and negatively regulates chaperone activity", J Biol Chem, 280, pp.8125~8133 (2005)
- Mori C, "Morphological analysis of germ cell apoptosis during postnatal testis development in normal and Hsp 70-2 knockout mice", Dev Dyn, 208, pp.125~136 (1997)
- Nagano R, "Reproliferation and relocation of mouse male germ cells (gonocytes) during prespermatogenesis", Anat Rec, 258, pp.210~1120 (2000)
- Rodriguez I, "An early and massive wave of germinal cell apoptosis is required for the development of functional spermatogenesis", EMBO J, 16, pp.2262~2270 (1997)
- Ryu SW, "Identification and characterization of human Fas associated factor 1, FAF1", Biochem Biophys Res Commun, 262, pp.388~394 (1999)
- Ryu SW, "Apoptosis induced by human Fas-associated factor 1, hFAF1, requires its ubiquitin homologous domain, but not the Fas-binding domain", Biochem Biophys Res Commun, 286, pp.1027~1032 (2001)
- Schwoebel ED, "The control of gene expression by regulated nuclear transport", Essays Biochem, 36, pp.105~113 (2000)
- Shinohara T, "Spermatogonial stem cell enrichment by multiparameter selection of mouse testis cells", Proc Natl Acad Sci USA, 통권 : 97, pp.8346~8351 (2000)
- Siomi H, "A nuclear localization domain in the hnRNP A1 protein", J Cell Biol, 129, pp.551~560 (1995)
- Sommer L, "Nuclear targeting of the transcription factor PTF1 is mediated by a protein subunit that does not bind to the PTF1 cognate sequence", Cell, 67, pp.987~994 (1991)
- Stein GS, "Nuclear microenvironments support physiological control of gene expression", Chromosome Res, 11, pp.527~536 (2003)
- Vergouwen RP, "Proliferative activity of gonocytes, Sertoli cells and interstitial cells during testicular development in mice", J Reprod Fertil, 93, pp.233~243 (1991)
- Wilkinson MF, "Multifunctional regulatory proteins that control gene expression in both the nucleus and the cytoplasm", Bioessays, 23, pp.775~787 (2001)
- Yoshimizu T, "Stage-specific tissue and cell interactions play key roles in mouse germ cell specification", Development, 128, pp.481~490 (2001)