Differential Expression of Cyclic AMP-Response Element Binding Protein Zhangfei (CREBZF) in the Mouse Testis during Postnatal Development
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Cyclic AMP-response element binding protein zhangfei (CREBZF), a member of ATF/CREB (activating tran-scription factor/ cAMP response element binding protein) family, regulates numerous cellular functions and development of cells by interacting transcription factors. This study discovered the expression pattern of CREBZF in seminiferous tubule of testes during the postnatal development of mice. In testis, CREBZF mRNA expression was the highest among other organs. Immunofluorescence analyses showed that the CREBZF was specifically expressed on spermatocyte but not in spermatogonia and Sertoli cells in seminiferous epithelium of mouse testis. Semi-quantitative polymerase chain reaction (PCR) analysis showed that CREBZF transcript level was significantly elevated during postnatal development of mouse testis. Confocal im-aging analysis indicated that the protein expression of CREBZF in seminiferous tubule remained low until postnatal day (PD) 14, and was dramatically increased in PD 21. Interestingly, only one type of the spermatocyte expressed CREBZF specifically among SCP3-positive spermatocytes. Taken together, these results suggest that CREBZF may be novel putative marker of the spermatocyte and regulate meiosis during postnatal development of mice.
Abstract
Cyclic AMP-response element binding protein zhangfei (CREBZF), a member of ATF/CREB (activating tran-scription factor/ cAMP response element binding protein) family, regulates numerous cellular functions and development of cells by interacting transcription factors. This study discovered the expression pattern of CREBZF in seminiferous tubule of testes during the postnatal development of mice. In testis, CREBZF mRNA expression was the highest among other organs. Immunofluorescence analyses showed that the CREBZF was specifically expressed on spermatocyte but not in spermatogonia and Sertoli cells in seminiferous epithelium of mouse testis. Semi-quantitative polymerase chain reaction (PCR) analysis showed that CREBZF transcript level was significantly elevated during postnatal development of mouse testis. Confocal im-aging analysis indicated that the protein expression of CREBZF in seminiferous tubule remained low until postnatal day (PD) 14, and was dramatically increased in PD 21. Interestingly, only one type of the spermatocyte expressed CREBZF specifically among SCP3-positive spermatocytes. Taken together, these results suggest that CREBZF may be novel putative marker of the spermatocyte and regulate meiosis during postnatal development of mice.
참고문헌 (25)
- Ahmed EA, "Staging of mouse seminiferous tubule cross-sections", Methods Mol Biol, 558, pp.263~277 (2009)
- Barnes BM, "The influence of hibernation on testis growth and spermatogenesis in the golden-mantled ground squirrel, Spermophilus lateralis", Biol Reprod, 35, pp.1289~1297 (1986)[DOI]
- Calvo A, "Characterization of the glycoconjugates of boar testis and epididymis", J Reprod Fertil, 120, pp.325~335 (2000)
- Clermont Y, "Renewal of spermatogonia in the rat", Dev Dyn, 93, pp.475~501 (1953)
- Clermont Y, "Quantitative study of the cell population of the seminiferous tubules in immature rats", Dev Dyn, 100, pp.241~267 (1957)
- Costoya JA, "Essential role of Plzf in maintenance of spermatogonial stem cells", Nature Genetics, 36, pp.653~659 (2004)[DOI]
- Drumond AL, "Spermatogonial morphology and kinetics during testis development in mice: A high-resolution light microscopy approach", Reproduction, 142, pp.145~155 (2011)[DOI]
- Franca LR, "The length of the cycle of seminiferous epithelium in goats (Capra hircus)", Tissue Cell, 31, pp.274~280 (1999)[DOI]
- Gassei K, "SALL4 expression in gonocytes and spermatogonial clones of postnatal mouse testes", PloS one, 8, pp.e53976~ (2013)[DOI]
- Griswold MD, "The central role of sertoli cells in spermatogenesis", Semin Cell Dev Biol, 9, pp.411~416 (1998)[DOI]
- Jagiello GM, "The hibernating male golden hamster as a model for studying homologue pairing, chiasma formation and disjunction during spermatogenesis", Biol Reprod, 35, pp.1037~1043 (1986)[DOI]
- Jang H, "SMILE inhibits BMP-2-induced expression of osteocalcin by suppressing the activity of the RUNX2 transcription factor in MC3T3E1 cells", Bone, 61, pp.10~18 (2014)[DOI]
- Jang H, "Small heterodimer partner-interacting leucine zipper protein inhibits adipogenesis by regulating peroxisome proliferator-activated receptor <TEX>${\gamma}$<", TEX> activity, 2015, pp.132, 49~54
- Jost A, "Early stages of testicular differentiation in the rat", Hum Genet, 58, pp.59~63 (1981)
- L'Hernault SW, "Spermatogenesis. WormBook : The online review of C", elegans biology, pp.1~14 (2006)
- 이정훈, "Seminiferous Epithelium Cycle and Developmental Stages of Spermatids in the Clethrionomys rufocanus", 발생과 생식, 17(2), pp.87~97 (2013), 한국발생생물학회
- Lin P, "CREBZF expression and hormonal regulation in the mouse uterus", Reprod Biol Endocrinol, 11, pp.110~ (2013)[DOI]
- Nakata H, "Quantitative analysis of the cellular composition in seminiferous tubules in normal and genetically modified infertile mice", J Histochem Cytochem, 63, pp.99~113 (2015)[DOI]
- Parua S, "Effect of an increase in environmental temperature on testicular androgenesis and spermatogenesis in toad (Bufo melanostictus) during hibernating season", Zoo Biol, 30, pp.681~688 (2011)[DOI]
- Pinart E, "Ultrastructural study of the boar seminiferous epithelium: Changes in cryptorchidism", J Morphol, 244, pp.190~202 (2000)[DOI]
- Tokuda M, "CDH1 is a specific marker for undifferentiated spermatogonia in mouse testes", Biol Reprod, 76, pp.130~141 (2007)[DOI]
- Yuan L, "The murine SCP3 gene is required for synaptonemal complex assembly, chromosome synapsis, and male fertility", Mol Cell, 5, pp.73~83 (2000)[DOI]
- Zhang R, "Effects of cyclic AMP response element binding protein-Zhangfei (CREBZF) on the unfolded protein response and cell growth are exerted through the tumor suppressor p 53", Cell Cycle, 13, pp.279~292 (2014)[DOI]
- Zhang R, "The effect of Zhangfei", CREBZF on cell growth, differentiation, apoptosis, migration, and the unfolded protein response in several canine osteosarcoma cell lines, 2015, pp.11, 22~
- Zhang Y, "Amino acid deprivation induces CREBZF", Zhangfei expression via an AARE-like element in the promoter, 2010, pp.391, 1352~1357
